公开(公告)号：US20210207078A1

公开(公告)日：2021-07-08

申请号：US17151871

申请日：2021-01-19

申请人： MANUS BIO, INC.

发明人： Aaron LOVE ,  Adel GHADERI ,  Jason Eric DONALD ,  Christine Nicole S. SANTOS ,  Ajikumar Parayil KUMARAN

IPC分类号： C12N1/20 ,  C12N1/16 ,  C12N1/26 ,  C12P5/00

摘要： Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.

公开(公告)号：US20240093258A1

公开(公告)日：2024-03-21

申请号：US18038067

申请日：2021-11-24

申请人： Manus Bio Inc.

发明人： Ajikumar PARAYIL KUMARAN ,  Christine Nicole S. SANTOS ,  Jason DONALD ,  Aaron LOVE ,  Yiying ZHENG ,  Adel GHADERI ,  Vineet SHASTRY ,  Lu CHEN ,  Christopher TOOMEY ,  Hannah LYNCH ,  Eric NIEMINEN

IPC分类号： C12P19/60 ,  C12N9/10 ,  C12N9/90

CPC分类号： C12P19/60 ,  C12N9/1051 ,  C12N9/90 ,  C12Y204/01017 ,  C12Y503/01009

摘要： In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.