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公开(公告)号：US20210207078A1
公开(公告)日：2021-07-08
申请号：US17151871
申请日：2021-01-19
申请人： MANUS BIO, INC.
发明人： Aaron LOVE , Adel GHADERI , Jason Eric DONALD , Christine Nicole S. SANTOS , Ajikumar Parayil KUMARAN
摘要： Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.
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公开(公告)号：US20210009968A1
公开(公告)日：2021-01-14
申请号：US16912374
申请日：2020-06-25
申请人： Manus Bio, Inc.
发明人： Jason Eric DONALD , Ajikumar Parayil KUMARAN , Aaron LOVE , Christopher TOOMEY , Christine Nicole S. SANTOS
摘要： In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
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公开(公告)号：US20230313249A1
公开(公告)日：2023-10-05
申请号：US18023912
申请日：2021-08-26
申请人： Manus Bio Inc.
发明人： Ajikumar PARAYIL KUMARAN , Christine Nicole S. SANTOS , Stephen SARRIA , Jason Eric DONALD , Yiying ZHENG , Liwei LI , Eric NIEMINEN , Michelle N. GOETTGE
CPC分类号： C12P17/181 , C12N15/52 , C12N9/001 , C12Y103/01092 , C12N9/0042 , C12N9/88 , C12Y402/03024
摘要： The present disclosure provides methods and compositions for producing artemisinic acid, dihydroartemisinic acid or artemisinin. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of artemisinic acid, dihydroartemisinic acid or artemisinin. The present disclosure further provides methods of making pharmaceutical products containing artemisinic acid, dihydroartemisinic acid or artemisinin.
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公开(公告)号：US20220090031A1
公开(公告)日：2022-03-24
申请号：US17495139
申请日：2021-10-06
申请人： Manus Bio Inc.
发明人： Jason Eric DONALD , Ajikumar Parayil KUMARAN , Aaron LOVE , Christopher TOOMEY , Christine Nicole S. SANTOS
摘要： In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
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公开(公告)号：US20240254521A1
公开(公告)日：2024-08-01
申请号：US18560260
申请日：2022-05-11
申请人： MANUS BIO INC.
发明人： Stephen SARRIA , Yiying ZHENG , Morgan FETHEROLF , Liwei LI , Ajikumar PARAYIL-KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD
CPC分类号： C12P7/26 , C12N9/0006 , C12N9/0042 , C12N9/0073 , C12N9/88 , C12N15/52 , C12P5/007 , C12Y101/01001 , C12Y106/02004 , C12Y114/13078 , C12Y402/03087
摘要： The present disclosure in various aspects provides engineered enzymes and encoding polynucleotides, as well as host cells and methods for making rotundone and other terpenoids. For example, in various aspects, the invention provides engineered α-Guaiene Synthase (αGS) and Guaiene Oxidase (GO) enzymes that increase biosynthesis of rotundone from farnesyl diphosphate, and in certain embodiments substantially reduce biosynthesis of side products such as α-Bulnesene or oxygenated side products. In still other aspects, the invention provides engineered terpene synthase enzymes for directing biosynthesis toward a desired product, to thereby improve product profiles and/or product titers from terpene synthase reactions.
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公开(公告)号：US20230042171A1
公开(公告)日：2023-02-09
申请号：US17785488
申请日：2020-12-16
申请人： Manus Bio Inc.
发明人： Michelle N. GOETTGE , Ryan N. PHILIPPE , Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Christopher FREI , Aaron LOVE , Kaitlin CHAMBERS , Joyce SAMSON , Christopher TOOMEY
摘要： The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog.V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.
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公开(公告)号：US20220162658A1
公开(公告)日：2022-05-26
申请号：US17546758
申请日：2021-12-09
申请人： Manus Bio Inc.
发明人： Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Mary Elizabeth FOWLER , Ryan N. PHILIPPE , Christopher Scott FREI , Aaron LOVE
IPC分类号： C12P19/18
摘要： In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
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公开(公告)号：US20200087692A1
公开(公告)日：2020-03-19
申请号：US16512874
申请日：2019-07-16
申请人： Manus Bio, Inc.
发明人： Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Mary Elizabeth FOWLER , Ryan N. PHILIPPE , Christopher Scott FREI , Aaron LOVE
IPC分类号： C12P19/18
摘要： In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
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