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公开(公告)号：US20240215615A1
公开(公告)日：2024-07-04
申请号：US18467362
申请日：2023-09-14
申请人： Manus Bio Inc.
发明人： Ryan PHILIPPE , Ajikumar Parayil KUMARAN , Jason DONALD , Krishna PATEL , Swati GUPTA , Ryan LIM , Liwei LI
CPC分类号： A23L2/60 , A23L27/36 , C12N9/0073 , C12N9/1048 , C12N9/1051 , C12P19/56 , C12Y114/13079 , C12Y204/01017 , A23V2002/00
摘要： The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
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公开(公告)号：US12012626B2
公开(公告)日：2024-06-18
申请号：US17546758
申请日：2021-12-09
申请人： Manus Bio Inc.
发明人： Ajikumar Parayil Kumaran , Christine Nicole S. Santos , Jason Eric Donald , Mary Elizabeth Fowler , Ryan N. Philippe , Christopher Scott Frei , Aaron Love
IPC分类号： C12P19/18
CPC分类号： C12P19/18
摘要： In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
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公开(公告)号：US11952608B2
公开(公告)日：2024-04-09
申请号：US17141758
申请日：2021-01-05
申请人： Manus Bio Inc.
发明人： Ajikumar Parayil Kumaran , Chin Giaw Lim , Liwei Li , Souvik Ghosh , Christopher Pirie , Anthony Qualley
CPC分类号： C12P7/26 , A01N27/00 , A23L27/13 , C12N9/0073 , C12P5/002 , C12Y114/13078 , A23V2002/00 , C07K2319/03
摘要： The present invention relates to methods for producing oxygenated terpenoids. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
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公开(公告)号：US20230399672A1
公开(公告)日：2023-12-14
申请号：US18034120
申请日：2021-11-02
发明人： Martin ZIEGLER , Ralf TAKORS , Joana SIEMEN , Michael LÖFFLER , Christine Nicole S. SANTOS , Ajikumar Parayil KUMARAN
CPC分类号： C12P21/02 , C12N15/52 , C12N15/70 , C12N9/0008 , C12Y102/07001 , C12N9/1051 , C12Y204/01 , C12N9/1205 , C12Y207/01002 , C12Y207/01032 , C12N9/90 , C12Y504/99009
摘要： The present invention in various aspects and embodiments provides engineered bacterial strains having a modified genome that provides advantages in maintenance and biosynthetic processes when cultured at large scale. The present invention further provides methods for large-scale fermentation using the bacterial strains. In various embodiments, the strain engineering yields a reduction in cellular responses to micro-environmental stimuli imposed in large scale bioreactors.
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公开(公告)号：US20230313249A1
公开(公告)日：2023-10-05
申请号：US18023912
申请日：2021-08-26
申请人： Manus Bio Inc.
发明人： Ajikumar PARAYIL KUMARAN , Christine Nicole S. SANTOS , Stephen SARRIA , Jason Eric DONALD , Yiying ZHENG , Liwei LI , Eric NIEMINEN , Michelle N. GOETTGE
CPC分类号： C12P17/181 , C12N15/52 , C12N9/001 , C12Y103/01092 , C12N9/0042 , C12N9/88 , C12Y402/03024
摘要： The present disclosure provides methods and compositions for producing artemisinic acid, dihydroartemisinic acid or artemisinin. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of artemisinic acid, dihydroartemisinic acid or artemisinin. The present disclosure further provides methods of making pharmaceutical products containing artemisinic acid, dihydroartemisinic acid or artemisinin.
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公开(公告)号：US20230180691A1
公开(公告)日：2023-06-15
申请号：US17981867
申请日：2022-11-07
CPC分类号： A01H3/04 , C12N1/12 , C12N1/14 , C12N1/16 , C12N1/20 , C12N15/1079 , C12Q1/025 , G01N33/5082 , G01N33/5097
摘要： The present invention relates to systems and methods for screening natural products such as secondary metabolites produced by engineered microbial strains.
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公开(公告)号：US20220090031A1
公开(公告)日：2022-03-24
申请号：US17495139
申请日：2021-10-06
申请人： Manus Bio Inc.
发明人： Jason Eric DONALD , Ajikumar Parayil KUMARAN , Aaron LOVE , Christopher TOOMEY , Christine Nicole S. SANTOS
摘要： In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
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公开(公告)号：US20210002678A1
公开(公告)日：2021-01-07
申请号：US16982916
申请日：2019-03-20
申请人： Manus Bio, Inc.
摘要： The present disclosure provides microbial cells and methods of producing FOPPA resulting from unique biosynthetic pathways, including biosynthetic pathways based on the phenylalanine/tyrosine biosynthetic branch and biosynthetic pathways based on bacteria metabolism. In particular, the present invention provides methods of producing FOPPA in microbial cells. These methods provide a low-cost, sustainable, and environmentally friendly source for FOPPA.
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公开(公告)号：US20200347425A1
公开(公告)日：2020-11-05
申请号：US16765196
申请日：2018-11-15
申请人： MANUS BIO, INC.
IPC分类号： C12P19/56
摘要： The present invention provides engineered cells and methods for making high purity steviol glycosides, including RebM. In some aspects, the present invention provides host cells, such as bacterial cells (including but not limited to E. coli), that are engineered to overexpress and/or delete or inactivate one or more steviol glycoside transport proteins. The bacterial cells selectively export RebM, or other specific combination of steviol glycosides, out of the cell to increase productivity and reduce production costs associated with downstream purification. Non-target steviol glycosides are not transported to the extracellular medium in significant amounts.
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公开(公告)号：US20190269157A1
公开(公告)日：2019-09-05
申请号：US16251993
申请日：2019-01-18
申请人： Manus Bio, Inc.
发明人： Ryan PHILIPPE , Ajikumar Parayil KUMARAN , Jason DONALD , Krishna PATEL , Swati GUPTA , Ryan LIM , Liwei LI
摘要： The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
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