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公开(公告)号：US20230042171A1
公开(公告)日：2023-02-09
申请号：US17785488
申请日：2020-12-16
申请人： Manus Bio Inc.
发明人： Michelle N. GOETTGE , Ryan N. PHILIPPE , Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Christopher FREI , Aaron LOVE , Kaitlin CHAMBERS , Joyce SAMSON , Christopher TOOMEY
摘要： The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog.V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.
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公开(公告)号：US20220364127A1
公开(公告)日：2022-11-17
申请号：US17729056
申请日：2022-04-26
申请人： MANUS BIO INC.
发明人： Ajikumar Parayil KUMARAN , Chin-Giaw LIM , Souvik GHOSH , Christopher PIRIE , Jason DONALD , Aaron LOVE , Hong NAN , Hsien-Chung TSENG , Christine Nicole S. SANTOS , Ryan PHILIPPE
摘要： In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
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公开(公告)号：US20210292798A1
公开(公告)日：2021-09-23
申请号：US17141758
申请日：2021-01-05
申请人： Manus Bio Inc.
发明人： Ajikumar Parayil KUMARAN , Chin Giaw LIM , Liwei LI , Souvik GHOSH , Christopher PIRIE , Anthony QUALLEY
摘要： The present invention relates to methods for producing oxygenated terpenoids. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
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公开(公告)号：US20210032669A1
公开(公告)日：2021-02-04
申请号：US16971740
申请日：2019-02-27
申请人： Manus Bio, Inc.
摘要： The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog, V. Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
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公开(公告)号：US20220251607A1
公开(公告)日：2022-08-11
申请号：US17729096
申请日：2022-04-26
申请人： MANUS BIO INC.
发明人： Ajikumar Parayil KUMARAN , Ryan LIM , Jason DONALD , Hsien-Chung TSENG , Christine Nicole S. SANTOS , Ryan PHILIPPE
摘要： The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
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公开(公告)号：US20220002764A1
公开(公告)日：2022-01-06
申请号：US17293230
申请日：2019-11-14
申请人： Manus Bio Inc.
IPC分类号： C12P7/42 , C12N9/12 , C12N15/52 , C12N1/16 , C12N1/32 , C12N1/20 , C12N9/02 , C12N9/10 , C12N9/88 , C12N9/00
摘要： Enzymes involved in cannabinoid biosynthesis are recombinantly expressed in a host cell. The host cell may be a prokaryote (e.g. Escherichia coli) or a eukaryote (e.g. Yarrowia lipolytica). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells.
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公开(公告)号：US20210207078A1
公开(公告)日：2021-07-08
申请号：US17151871
申请日：2021-01-19
申请人： MANUS BIO, INC.
发明人： Aaron LOVE , Adel GHADERI , Jason Eric DONALD , Christine Nicole S. SANTOS , Ajikumar Parayil KUMARAN
摘要： Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.
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公开(公告)号：US20180245103A1
公开(公告)日：2018-08-30
申请号：US15888573
申请日：2018-02-05
申请人： MANUS BIO, INC.
发明人： Ajikumar Parayil KUMARAN , Chin-Giaw LIM , Souvik GHOSH , Christopher PIRIE , Jason DONALD , Aaron LOVE , Hong NAN , Hsien-Chung TSENG , Christine Nicole S. SANTOS , Ryan PHILIPPE
摘要： In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
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公开(公告)号：US20220162658A1
公开(公告)日：2022-05-26
申请号：US17546758
申请日：2021-12-09
申请人： Manus Bio Inc.
发明人： Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Mary Elizabeth FOWLER , Ryan N. PHILIPPE , Christopher Scott FREI , Aaron LOVE
IPC分类号： C12P19/18
摘要： In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
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公开(公告)号：US20210261992A1
公开(公告)日：2021-08-26
申请号：US17251922
申请日：2019-06-12
申请人： Manus Bio, Inc.
摘要： The present invention is related to enzymatic pathways for production of carminic acid, host cells capable of production of carminic acid, and methods for the production of carminic acid and related compounds.
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