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1.发明授权
公开(公告)号：US5478369A
公开(公告)日：1995-12-26
申请号：US103739
申请日：1993-08-02
IPC分类号： A01H1/00 , A01H1/02 , A01H5/00 , A01H5/10 , C07K14/415 , C12N5/04 , C12N9/02 , C12N15/09 , C12N15/29 , C12N15/82 , C12N15/11
CPC分类号： A01H1/02 , C07K14/415 , C12N15/82 , C12N15/8289 , C12N5/04 , C12N9/0004 , Y10S47/01
摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Use of the nucleotide sequences to mediate fertility in plants is also described. In one such method, an inducible promoter is used to regulate expression of the DNA molecule. The control sequences are modified so that it is normally "off" and as a result the plants are male sterile. When it is desired to reproduce the male sterile plants, male fertility is restored by treating the plants with a non-phytotoxic chemical which induces expression of the critical gene.
摘要翻译： 描述了介导植物中雄性生育力的核苷酸序列，其中阐述了DNA分子和氨基酸序列。 还描述了使用核苷酸序列来介导植物中的生育力。 在一种这样的方法中，诱导型启动子用于调节DNA分子的表达。 控制序列被修饰，使其通常是“关闭”的，因此植物是雄性不育的。 当需要繁殖雄性不育植物时，通过用诱导关键基因表达的非植物毒性化学品处理植物来恢复雄性生育力。
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公开(公告)号：US06297426B1
公开(公告)日：2001-10-02
申请号：US09059958
申请日：1998-04-14
IPC分类号： A01H500
CPC分类号： C12N15/8289 , C07K14/415
摘要： A controllably constitutively female sterile plant is provided. A gene critical to female fertility is identified and the native gene inactivated. The gene is cloned, linked to an inducible promoter and inserted into the plant. The result is a plant that is constitutively female sterile with inducible female fertility.
摘要翻译： 提供了可控的组成型雌性不育植物。 鉴定出对雌性生殖力至关重要的基因，天然基因失活。 将该基因克隆，连接到诱导型启动子并插入植物中。 结果是一种具有诱导性雌性生殖力的组成型雌性不育的植物。
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公开(公告)号：US06265640B1
公开(公告)日：2001-07-24
申请号：US09211416
申请日：1998-12-10
IPC分类号： A01H500
CPC分类号： A01H1/02 , C07K14/415 , C12N5/04 , C12N9/0004 , C12N15/82 , C12N15/8289 , Y10S47/01
摘要： Fertility in a plant is controlled by inactivating a gene critical to fertility, and inserting into the plant the criticial gene linked to an inducible promoter.
摘要翻译： 通过灭活对生殖力至关重要的基因并将与诱导型启动子连接的突变基因插入植物来控制植物中的生育力。
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公开(公告)号：US5859341A
公开(公告)日：1999-01-12
申请号：US482714
申请日：1995-06-07
IPC分类号： A01H1/00 , A01H1/02 , A01H5/00 , A01H5/10 , C07K14/415 , C12N5/04 , C12N9/02 , C12N15/09 , C12N15/29 , C12N15/82 , A01H4/00 , C12N15/00 , C12N15/05
CPC分类号： A01H1/02 , C07K14/415 , C12N15/82 , C12N15/8289 , C12N5/04 , C12N9/0004 , Y10S47/01
摘要： Fertility in a plant is controlled by inactivating a gene critical to fertility, and inserting into the plant the criticial gene linked to an inducible promoter.
摘要翻译： 通过灭活对生殖力至关重要的基因并将与诱导型启动子连接的突变基因插入植物来控制植物中的生育力。
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公开(公告)号：US5850014A
公开(公告)日：1998-12-15
申请号：US485845
申请日：1995-06-07
IPC分类号： A01H1/00 , A01H1/02 , A01H5/00 , A01H5/10 , C07K14/415 , C12N5/04 , C12N9/02 , C12N15/09 , C12N15/29 , C12N15/82 , C12N15/00 , A01H1/06 , A01H4/00 , C12N5/00
CPC分类号： A01H1/02 , C07K14/415 , C12N15/82 , C12N15/8289 , C12N5/04 , C12N9/0004 , Y10S47/01
摘要： Fertility in a plant is controlled by inactivating a gene critical to fertility, and inserting into the plant the critical gene linked to an inducible promoter.
摘要翻译： 通过灭活对生殖力至关重要的基因并将与诱导型启动子连接的关键基因插入植物来控制植物中的生育力。
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公开(公告)号：US5824524A
公开(公告)日：1998-10-20
申请号：US474404
申请日：1995-06-07
IPC分类号： C07K14/415 , C12N15/82 , C12N15/00 , A01H1/00 , C12N15/05
CPC分类号： C07K14/415 , C12N15/8289 , Y10S47/01
摘要： Fertility in a plant is controlled by inactivating a gene critical to fertility, and inserting into the plant the criticial gene linked to an inducible promoter.
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7.发明授权
公开(公告)号：US5432068A
公开(公告)日：1995-07-11
申请号：US848433
申请日：1992-03-09
IPC分类号： A01H1/00 , A01H1/02 , A01H5/00 , A01H5/10 , C07K14/415 , C12N5/04 , C12N9/02 , C12N15/09 , C12N15/29 , C12N15/55 , C12N15/82 , C12N15/00
CPC分类号： C12N9/0004 , A01H1/02 , C07K14/415 , C12N15/82 , C12N15/8289 , C12N5/04
摘要： An inducible promoter is used to regulate expression of a gene which is known to be critical to male fertility. The selected gene's control sequences are modified so that it is normally "off" and as a result the plants are male sterile. When it is desired to reproduce the male sterile plants, male fertility is restored by treating the plants with a non-phytotoxic chemical which induces expression of the critical gene. A critical gene is one which affects flavonol production and in particular compounds of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.7, and R.sub.8, are hydrogen, hydroxyl or alkoxy having from 1 to 4 carbon atoms. Particularly preferred flavonols include galangin, kaempferol, iso-rhamnetin, quercetin and morin.
摘要翻译： 可诱导的启动子用于调节已知对雄性生殖力至关重要的基因的表达。 所选择的基因的控制序列被修饰，使其通常是“关闭”的，因此植物是雄性不育的。 当需要繁殖雄性不育植物时，通过用诱导关键基因表达的非植物毒性化学品处理植物来恢复雄性生育力。 关键基因是影响黄酮醇生成的基因，特别是下式的化合物：其中R 1，R 2，R 3，R 4，R 5，R 7和R 8是氢，羟基或具有1至4个碳原子的烷氧基。 特别优选的黄酮醇包括高良姜素，山奈酚，异鼠李素，槲皮素和mor。。
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公开(公告)号：US06037523A
公开(公告)日：2000-03-14
申请号：US880499
申请日：1997-06-23
IPC分类号： A01H5/00 , C07K14/34 , C12N5/10 , C12N9/10 , C12N9/22 , C12N9/24 , C12N15/09 , C12N15/29 , C12N15/82 , C12N15/00 , A01H1/06 , A01H4/00 , C12N5/00
CPC分类号： C12Y302/01031 , C07K14/34 , C12N15/8231 , C12N15/8289 , C12N9/1037 , C12N9/22 , C12N9/2402 , C12N9/93 , C12Y302/01039 , C12Y602/01003
摘要： The present invention relates to an isolated nucleic acid sequence encoding the Ms45 male tissue-preferred regulatory region. In one aspect this invention relates the use of this male tissue-preferred regulatory region in mediating fertility. An example of such use is the production of hybrid seed such as in a male sterility system. The Ms45 male tissue-preferred regulatory region can be operably linked with exogenous genes, such as those encoding cytotoxins, complementary nucleotidic units and inhibitory molecules. This invention also relates to plant cells, plant tissue and differentiated plants which contain the regulatory region in this invention.
摘要翻译： 本发明涉及编码Ms45雄性组织优选调节区的分离的核酸序列。 一方面，本发明涉及这种雄性组织优选的调节区在介导生育力中的用途。 这种用途的一个例子是杂交种子的生产，例如在雄性不育系统中。 可将Ms45雄性组织优选的调节区域与外源基因（例如编码细胞毒素，互补核苷酸单元和抑制性分子的那些）可操作地连接。 本发明还涉及在本发明中含有调节区的植物细胞，植物组织和分化植物。
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9.发明授权MSCA1 nucleotide sequences impacting plant male fertility and method of using same 有权影响植物雄性生育力的MSCA1核苷酸序列及其使用方法公开(公告)号：US08927805B2
公开(公告)日：2015-01-06
申请号：US13032335
申请日：2011-02-22
申请人： Marc C. Albertsen , Tim Fox , Mary Trimnell , Yongzhong Wu , Keith Lowe
发明人： Marc C. Albertsen , Tim Fox , Mary Trimnell , Yongzhong Wu , Keith Lowe
CPC分类号： C12N15/8289 , C07K14/415
摘要： Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.
摘要翻译： 描述了在植物中对雄性生育力至关重要的Msca1基因的核苷酸序列，其中阐述了DNA分子和氨基酸序列。 还鉴定了启动子序列及其必需区域。 核苷酸序列可用于影响植物中的雄性生殖力。
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10.发明授权MSCA1 nucleotide sequences impacting plant male fertility and method of using same 有权影响植物雄性生育力的MSCA1核苷酸序列及其使用方法公开(公告)号：US08648228B2
公开(公告)日：2014-02-11
申请号：US13022336
申请日：2011-02-07
申请人： Marc C. Albertsen , Tim Fox , Mary Trimnell , Yongzhong Wu , Keith Lowe , Bailin Li , Marianna Faller
发明人： Marc C. Albertsen , Tim Fox , Mary Trimnell , Yongzhong Wu , Keith Lowe , Bailin Li , Marianna Faller
CPC分类号： C12N15/8231 , C07K14/415 , C12N15/8289
摘要： Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.
摘要翻译： 描述了在植物中对雄性生育力至关重要的Msca1基因的核苷酸序列，其中阐述了DNA分子和氨基酸序列。 还鉴定了启动子序列及其必需区域。 核苷酸序列可用于影响植物中的雄性生殖力。
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