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公开(公告)号：US20090209736A1
公开(公告)日：2009-08-20
申请号：US12304840
申请日：2007-06-18
申请人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , Laurent David , Margit Holzer
发明人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , Laurent David , Margit Holzer
CPC分类号： B01D15/1871 , B01D15/1828 , B01D15/361 , B01D15/3804
摘要： The subject of the invention is separation on a solid support by multi-column sequential selective retention in order to separate a product of interest from a solution containing such a product of interest, by passing this solution over a fixed chromatographic resin bed comprising at least three zones, liquid flow means being placed between adjacent zones and between the last zone and the first zone, this process comprising several sequences, each sequence comprising at least one step chosen from an absorption step, a rinsing step and a desorption step, carried out either simultaneously or not, each following sequence is carried out by the displacement of the fronts in the zones towards the downstream end substantially of the same increment before the periodic displacement of the points of introduction and of withdrawal, characterized in that the process includes a sub-sequence without injection of the charge.
摘要翻译： 本发明的主题是通过多柱顺序选择性保留在固体支持物上进行分离，以便通过将该溶液通过固定的色谱树脂床分离感兴趣的产物与含有目的产物的溶液，所述固定支架包含至少三个 区域，液体流动装置被放置在相邻区域之间以及最后区域和第一区域之间，该方法包括几个序列，每个序列包括至少一个选自吸收步骤，漂洗步骤和解吸步骤的步骤， 通过在引入点和撤出点的周期性位移之前，通过在区域中的前端向下游端基本上相同增量的位移来执行每个后续顺序，其特征在于， 序列而不注入电荷。
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公开(公告)号：US20120214966A1
公开(公告)日：2012-08-23
申请号：US13448959
申请日：2012-04-17
申请人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , David Laurent , Margit Holzer
发明人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , David Laurent , Margit Holzer
CPC分类号： B01D15/1871 , B01D15/1828 , B01D15/361 , B01D15/3804
摘要： A process is disclosed for separating biomolecules from an aqueous solution containing the biomolecules and impurities, having different affinities and/or interactions with a solid support. The solution is passed over a fixed bed of chromatographic resin containing at least three zones, with flow of liquid being arranged between adjacent zones and between a last and first zone. Each of several sequences includes at least an adsorption stage, a rinsing stage, or a desorption stage, with each subsequent sequence being carried out by a downstream displacement of fronts in the zones by approximately the same increment before the periodical displacement of the introduction and withdrawal points.
摘要翻译： 公开了一种从含有生物分子和杂质的水溶液中分离生物分子的方法，其与固体支持物具有不同的亲和力和/或相互作用。 该溶液通过含有至少三个区域的固定床层析树脂，其中液体流被布置在相邻区域之间并且在最后和第一区域之间。 几个序列中的每一个至少包括吸附阶段，漂洗阶段或解吸阶段，每个随后的顺序通过在引入和撤出的周期性位移之前在区域中的前沿的下游位移大致相同的增量来执行 积分
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公开(公告)号：US08465649B2
公开(公告)日：2013-06-18
申请号：US13448959
申请日：2012-04-17
申请人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , David Laurent , Margit Holzer
发明人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , David Laurent , Margit Holzer
IPC分类号： B01D15/08
CPC分类号： B01D15/1871 , B01D15/1828 , B01D15/361 , B01D15/3804
摘要： A process is disclosed for separating biomolecules from an aqueous solution containing the biomolectules and impurities, having different affinities and/or interactions with a solid support. The solution is passed over a fixed bed of chromatographic resin containing at least three zones, with flow of liquid being arranged between adjacent zones and between a last and first zone. Each of several sequences includes at least an adsorption stage, a rinsing stage, or a desorption stage, with each subsequent sequence being carried out by a downstream displacement of fronts in the zones by approximately the same increment before the periodical displacement of the introduction and withdrawal points.
摘要翻译： 公开了一种从含有生物电解质和杂质的水溶液中分离生物分子的方法，其具有与固体支持物的不同亲和力和/或相互作用。 该溶液通过含有至少三个区域的固定床层析树脂，其中液体流被布置在相邻区域之间并且在最后和第一区域之间。 几个序列中的每一个至少包括吸附阶段，漂洗阶段或解吸阶段，每个随后的顺序通过在引入和撤出的周期性位移之前在区域中的前沿的下游位移大致相同的增量来执行 积分
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公开(公告)号：US08182696B2
公开(公告)日：2012-05-22
申请号：US12304840
申请日：2007-06-18
申请人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , David Laurent , Margit Holzer
发明人： Marc-André Theoleyre , Stanislas Baudouin , Annick Merrien , Eric Valery , Olivier Ludemann-Hombourger , David Laurent , Margit Holzer
IPC分类号： B01D15/08
CPC分类号： B01D15/1871 , B01D15/1828 , B01D15/361 , B01D15/3804
摘要： A process is disclosed for separating biomolecules from an aqueous solution containing the biomolectules and impurities, having different affinities and/or interactions with a solid support. The solution is passed over a fixed bed of chromatographic resin containing at least three zones, with flow of liquid being arranged between adjacent zones and between a last and first zone. Each of several sequences includes at least an adsorption stage, a rinsing stage, or a desorption stage, with each subsequent sequence being carried out by a downstream displacement of fronts in the zones by approximately the same increment before the periodical displacement of the introduction and withdrawal points.
摘要翻译： 公开了一种从含有生物电解质和杂质的水溶液中分离生物分子的方法，其具有与固体支持物的不同亲和力和/或相互作用。 该溶液通过含有至少三个区域的固定床层析树脂，其中液体流被布置在相邻区域之间并且在最后和第一区域之间。 几个序列中的每一个至少包括吸附阶段，漂洗阶段或解吸阶段，每个随后的顺序通过在引入和撤出的周期性位移之前在区域中的前沿的下游位移大致相同的增量来执行 积分
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