利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

76 条记录 (耗时 0.016 秒)

    Process for the extraction, purification and enzymatic modification of soy 7S globulin α' subunit for use as hypocholesterolemizing agent
    1.
    发明授权
    Process for the extraction, purification and enzymatic modification of soy 7S globulin α' subunit for use as hypocholesterolemizing agent 有权
    大豆7S球蛋白α'亚基的提取，纯化和酶修饰方法，用作降血胆固醇剂

    公开(公告)号：US07465467B2

    公开(公告)日：2008-12-16

    申请号：US10502582

    申请日：2003-01-27

    申请人： Marcello Duranti ,  Paolo Morazzoni

    发明人： Marcello Duranti ,  Paolo Morazzoni

    IPC分类号： A61K36/48

    CPC分类号： A61K38/168 ,  A23J1/14 ,  A23J3/16 ,  A23L33/185 ,  Y02E50/17

    摘要： A process for the extraction, purification and enzymatic modification of β-conglycinin α′ subunit, wherein β-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the α′ subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).

    摘要翻译： β-伴大豆球蛋白α'亚基的提取，纯化和酶促修饰的方法，其中β-伴大豆球蛋白从地面选择性提取，脱脂大豆，然后用含水乙醇处理沉淀; 然后将富集的级分在变性剂条件下进行金属亲和层析（MAC），以获得用胰凝乳蛋白酶处理的α'亚基，然后进一步进行MAC以回收该多肽的氨基末端区域（MW 28,000Da） 。

    Process for the extraction, purification and enzymatic modification of soy 7S globulin alpha' subunit for use as hypocholesterolemizing agent
    5.
    发明授权
    Process for the extraction, purification and enzymatic modification of soy 7S globulin alpha' subunit for use as hypocholesterolemizing agent 有权
    大豆7S球蛋白α'亚基的提取，纯化和酶修饰方法，用作降血胆固醇剂

    公开(公告)号：US08257758B2

    公开(公告)日：2012-09-04

    申请号：US13013966

    申请日：2011-01-26

    申请人： Marcello Duranti ,  Paolo Morazzoni

    发明人： Marcello Duranti ,  Paolo Morazzoni

    IPC分类号： A61K36/48

    CPC分类号： A61K38/168 ,  A23J1/14 ,  A23J3/16 ,  A23L33/185 ,  Y02E50/17

    摘要： A purified amino-terminal polypeptide fragment of the soy β-conglycinin α′ subunit is prepared by selective extraction of defatted soy with an aqueous solution of sodium bisulfite, precipitation with ethanol, and Metal Affinity Chromatography (MAC) under denaturing conditions to obtain the α′ subunit. The α′ subunit is then enzymatically treated with chymotrypsin and subjected to further MAC to recover the amino-terminal fragment of the polypeptide (MW 28,000 Da).

    摘要翻译： 通过在变性条件下用亚硫酸氢钠水溶液，用乙醇沉淀和金属亲和层析（MAC）选​​择性提取脱脂大豆来制备大豆和大豆球蛋白α'亚基的纯化的氨基末端多肽片段，得到 α'亚基。 然后用胰凝乳蛋白酶对α'亚基进行酶处理，并进一步进行MAC以回收多肽的氨基末端片段（MW 28,000Da）。

    Use of lupin conglutin for the treatment of type II diabetes
    6.
    发明授权
    Use of lupin conglutin for the treatment of type II diabetes 有权
    使用羽扇豆凝集素治疗II型糖尿病

    公开(公告)号：US07323441B2

    公开(公告)日：2008-01-29

    申请号：US10545103

    申请日：2004-02-06

    申请人： Paolo Morazzoni ,  Marcello Duranti

    发明人： Paolo Morazzoni ,  Marcello Duranti

    IPC分类号： A61K38/00 ,  C07K14/00

    CPC分类号： A61K38/168 ,  A61K36/23 ,  A61K36/48

    摘要： A method for the use of lupin conglutin gamma or of proteins showing homology higher than 50% with lupin conglutin gamma, for the preparation of a medicament, food supplements or foods for the treatment of type II diabetes, pharmaceutical and nutritional compositions containing lupin conglutin gamma, and the use of lupin conglutin gamma as therapeutical agent, in particular as hypoglycemizing agent. Lupin conglutin gamma may be used in pure form or in form of extracts, mixtures or concentrates.

    摘要翻译： 使用羽扇豆凝集素γ或与羽扇豆凝集素γ显示同源性高于50％的蛋白质的方法，用于制备用于治疗II型糖尿病的药物，食品补充剂或食品，含有羽扇豆凝集素γ的药物和营养组合物 ，以及使用羽扇豆凝集素γ作为治疗剂，特别是低血糖剂。 羽扇豆凝集素γ可以以纯形式或以提取物，混合物或浓缩物的形式使用。

    Use of lupin conglutin for the treatment of type II diabetes
    7.
    发明申请
    Use of lupin conglutin for the treatment of type II diabetes 有权
    使用羽扇豆凝集素治疗II型糖尿病

    公开(公告)号：US20060142185A1

    公开(公告)日：2006-06-29

    申请号：US10545103

    申请日：2004-02-06

    申请人： Paolo Morazzoni ,  Marcello Duranti

    发明人： Paolo Morazzoni ,  Marcello Duranti

    IPC分类号： A61K38/16 ,  C07K14/415

    CPC分类号： A61K38/168 ,  A61K36/23 ,  A61K36/48

    摘要： A method for the use of lupin conglutin gamma or of proteins showing homology higher than 50% with lupin conglutin gamma, for the preparation of a medicament, food supplements or foods for the treatment of type II diabetes, pharmaceutical and nutritional compositions containing lupin conglutin gamma, and the use of lupin conglutin gamma as therapeutical agent, in particular as hypoglycemizing agent. Lupin conglutin gamma may be used in pure form or in form of extracts, mixtures or concentrates.

    摘要翻译： 使用羽扇豆凝集素γ或与羽扇豆凝集素γ显示同源性高于50％的蛋白质的方法，用于制备用于治疗II型糖尿病的药物，食品补充剂或食品，含有羽扇豆凝集素γ的药物和营养组合物 ，以及使用羽扇豆凝集素γ作为治疗剂，特别是低血糖剂。 羽扇豆凝集素γ可以以纯形式或以提取物，混合物或浓缩物的形式使用。

    Process for the extraction, purification and enzymatic modification of soy 7S globulin α′ subunit for use as hypocholesterolemizing agent
    9.
    发明授权
    Process for the extraction, purification and enzymatic modification of soy 7S globulin α′ subunit for use as hypocholesterolemizing agent 有权
    大豆7S球蛋白α'亚基的提取，纯化和酶促修饰方法用作低胆固醇血症药

    公开(公告)号：US07901718B2

    公开(公告)日：2011-03-08

    申请号：US12104473

    申请日：2008-04-17

    申请人： Marcello Duranti ,  Paolo Morazzoni

    发明人： Marcello Duranti ,  Paolo Morazzoni

    IPC分类号： A61K36/48

    CPC分类号： A61K38/168 ,  A23J1/14 ,  A23J3/16 ,  A23L33/185 ,  Y02E50/17

    摘要： A process for the extraction, purification and enzymatic modification of β-conglycinin α′ subunit, wherein β-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the α′ subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).

    摘要翻译： 一种用于大豆球蛋白α'亚基的提取，纯化和酶促修饰的方法，其中将大豆球蛋白从地面选择性提取，脱脂大豆，然后用含水乙醇处理沉淀; 然后将富集的级分在变性条件下进行金属亲和层析（MAC），以获得用胰凝乳蛋白酶处理的α'亚基，然后进一步进行MAC以回收该多肽的氨基末端区域（MW 28,000Da） 。