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公开(公告)号:US5447864A
公开(公告)日:1995-09-05
申请号:US120530
申请日:1993-09-14
申请人: Margaret P. Raybuck , Michael K. Kenrick , David A. Parry , Andrew L. Bertera , John G. Anson , Nicola M. Williamson
发明人: Margaret P. Raybuck , Michael K. Kenrick , David A. Parry , Andrew L. Bertera , John G. Anson , Nicola M. Williamson
CPC分类号: C12N15/1017 , C12M47/06 , C12N1/06 , Y10S435/803 , Y10S435/82 , Y10T436/2525 , Y10T436/25375 , Y10T436/255
摘要: A method of separating cell nuclei from cells comprises: treating a fluid containing whole cells so as to selectively lyse the cytoplasmic membrane together with a small proportion of the nuclear membranes but leaving a large proportion of the cell nuclei intact; applying the treated fluid to a membrane whereby a mesh of DNA from the lysed nuclei is formed on the surface and captures intact cell nuclei. A device for use in the method is described.
摘要翻译: 从细胞中分离细胞核的方法包括:处理含有全细胞的流体,以便与少量的核膜一起选择性裂解细胞质膜,但是留下大部分细胞核完整; 将处理的流体施加到膜上,由此在表面上形成来自裂解的核的DNA网,并捕获完整的细胞核。 描述了用于该方法的装置。
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公开(公告)号:US5556598A
公开(公告)日:1996-09-17
申请号:US119649
申请日:1993-09-13
IPC分类号: B01D29/01 , B01D15/00 , B01D63/08 , B01J20/281 , B01L3/02 , G01N1/00 , G01N1/10 , G01N1/40 , G01N30/52 , G01N30/60 , G01N33/48 , G01N33/543 , G01N35/04 , G01N35/10
CPC分类号: B01J20/281 , B01D15/3804 , B01L3/0275 , G01N30/48 , G01N33/54366 , B01J2220/54 , G01N1/405 , G01N2030/527 , G01N2035/0434 , G01N2035/1053 , G01N2035/1055 , G01N30/60
摘要: A device for capturing a component present in a fluid 18 a pipette tip having a rearward end 15 adapted to be fitted on a micro-pipette, and a forward end 12 with a membrane 17, adapted to bind the component, extending across the pipette tip.
摘要翻译: 用于捕获存在于流体18中的组分的装置,具有适于装配在微量移液管上的后端15的移液管尖端以及具有膜17的前端12,其适于结合部件,延伸穿过移液管尖端 。
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