公开(公告)号：US08158423B2

公开(公告)日：2012-04-17

申请号：US12525983

申请日：2008-02-05

申请人： Hidehiro Ozawa ,  Mariko Yamaki ,  Makoto Asashima ,  Satoshi Ebina

发明人： Hidehiro Ozawa ,  Mariko Yamaki ,  Makoto Asashima ,  Satoshi Ebina

IPC分类号： C12N15/02

CPC分类号： G01N33/5082 ,  C12N5/0627 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2506/02 ,  C12N2506/1361 ,  C12N2533/54

摘要： A method of making a hair, including a step of culturing an undifferentiated cell of a mammal to produce an embryoid body and a step of further culturing the embryoid body is provided, wherein the culturing step is to culture the embryoid body on a three-dimensional matrix for 5 to 12 days. Furthermore, a biological material obtainable by the method of making a hair as described above is provided. Moreover, a biological material for a screening system of evaluating a medical product or the like, obtainable by utilizing the method of making a hair as described above is provided.

摘要翻译： 提供了一种制备头发的方法，包括培养哺乳动物的未分化细胞以产生胚状体的步骤和进一步培养胚状体的步骤，其中培养步骤是将胚状体在三维培养 基质5至12天。 此外，提供了可通过如上所述制造头发的方法获得的生物材料。 此外，提供了通过利用上述制造头发的方法获得的用于评估医疗产品等的筛选系统的生物材料。

公开(公告)号：US20100304479A1

公开(公告)日：2010-12-02

申请号：US12525983

申请日：2008-02-05

申请人： Hidehiro Ozawa ,  Mariko Yamaki ,  Makoto Asashima ,  Satoshi Ebina

发明人： Hidehiro Ozawa ,  Mariko Yamaki ,  Makoto Asashima ,  Satoshi Ebina

IPC分类号： C12N5/0735

CPC分类号： G01N33/5082 ,  C12N5/0627 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2506/02 ,  C12N2506/1361 ,  C12N2533/54

摘要： A method of making a hair, including a step of culturing an undifferentiated cell of a mammal to produce an embryoid body and a step of further culturing the embryoid body is provided, wherein the culturing step is to culture the embryoid body on a three-dimensional matrix for 5 to 12 days. Furthermore, a biological material obtainable by the method of making a hair as described above is provided. Moreover, a biological material for a screening system of evaluating a medical product or the like, obtainable by utilizing the method of making a hair as described above is provided.

摘要翻译： 提供了一种制备头发的方法，包括培养哺乳动物的未分化细胞以产生胚状体的步骤和进一步培养胚状体的步骤，其中培养步骤是将胚状体在三维培养 基质5至12天。 此外，提供了可通过如上所述制造头发的方法获得的生物材料。 此外，提供了通过利用上述制造头发的方法获得的用于评估医疗产品等的筛选系统的生物材料。

公开(公告)号：US20100015199A1

公开(公告)日：2010-01-21

申请号：US12439053

申请日：2007-08-27

申请人： Mariko Yamaki ,  Hidehiro Ozawa ,  Satoshi Ebina ,  Makoto Asashima

发明人： Mariko Yamaki ,  Hidehiro Ozawa ,  Satoshi Ebina ,  Makoto Asashima

IPC分类号： A61L27/54 ,  C12N5/00 ,  A61K35/12

CPC分类号： C12N5/0606 ,  A61L27/3834 ,  C12N5/0603 ,  C12N2533/54

摘要： The present invention provides a method for suppressing generation of a teratoma of an undifferentiated cell, preferably an ES cell. A biological material obtainable by the method and a therapeutic method for transplanting the biological material into a subject are also provided.An undifferentiated cell, in particular, preferably an ES cell is cultured to produce an increased embryoid body-like and the embryoid body-like is cultured for at least one week on, and adhered to, a three-dimensional solid type-I collagen carrier or an analogue thereof. Furthermore, a biological material is obtained thereby and the biological material is transplanted into a mammalian subject.

摘要翻译： 本发明提供抑制未分化细胞，优选ES细胞的畸胎瘤的产生的方法。 还提供了通过该方法获得的生物材料和用于将生物材料移植到受试者中的治疗方法。 培养未分化细胞，特别优选ES细胞以产生增加的类胚体，并将胚状体样培养至少一周，并附着于三维固体I型胶原载体上 或其类似物。 此外，由此获得生物材料，将生物材料移植到哺乳动物受试者中。

公开(公告)号：US20100093080A1

公开(公告)日：2010-04-15

申请号：US11997077

申请日：2006-07-25

申请人： Mariko Yamaki ,  Hidehiro Ozawa ,  Satoshi Ebina

发明人： Mariko Yamaki ,  Hidehiro Ozawa ,  Satoshi Ebina

IPC分类号： C12N5/071

CPC分类号： A61L27/3865 ,  A61L27/3804 ,  A61L27/3886 ,  C12N5/0654 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2506/02

摘要： Disclosed is a novel method for formation of a tooth by producing a chimera embryoid body using an undifferentiated cell and a dental mesenchymal cell derived from a mammal of the same species as that of the target mammal and then cultivating the chimera embryoid body on a three-dimensional matrix. A tooth is formed by co-cultivating an undifferentiated cell and a dental mesenchymal cell derived from a mammal of the same species as that of the target mammal in the presence of an induction factor to produce a chimera embryoid body, and then cultivating the chimera embryoid body on a three-dimensional matrix. The cultivation of the chimera embryoid body on the three-dimensional matrix is performed either by cultivating the chimera embryoid body in a serum culture medium without the induction factor for three days; or cultivating the chimera embryoid body in a culture medium supplemented with the induction factor for two days, and further cultivating the chimera embryoid body in a serum culture medium without the induction factor for three days. The undifferentiated cell is at least one cell selected from all stem cells including an ES cell. The mammal is one selected from all mammals. The induction factor is activin, bone morphogenic protein 4, insulin growth factor 1, fibroblast growth factor 2, or transforming growth factor β1.

摘要翻译： 公开了一种通过使用未分化细胞和源自与目标哺乳动物相同种类的哺乳动物的牙间充质细胞产生嵌合体胚状体，然后在三维细胞上培养嵌合体胚状体来形成牙齿的新方法， 三维矩阵。 通过在诱导因子存在下共培养来自与哺乳动物相同种类的哺乳动物的未分化细胞和牙间充质细胞以形成嵌合体胚状体，然后培养嵌合体胚状体形成牙齿 身体在三维矩阵上。 三维基质上的嵌合体胚状体的培养是通过在没有诱导因子的血清培养基中培养嵌合体胚状体三天进行的; 或在补充有诱导因子的培养基中培养嵌合体胚状体两天，并且在无诱导因子的血清培养基中进一步培养嵌合体胚状体三天。 未分化细胞是选自包括ES细胞的所有干细胞中的至少一种细胞。 哺乳动物是选自所有哺乳动物的哺乳动物。 诱导因子是激活素，骨形态发生蛋白4，胰岛素生长因子1，成纤维细胞生长因子2或转化生长因子1。