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公开(公告)号：US07112441B2
公开(公告)日：2006-09-26
申请号：US10233506
申请日：2002-09-04
IPC分类号： C12N5/02
CPC分类号： C12M27/10 , C12M35/04 , C12N5/0062
摘要： In order to grow an object to be cultivated or grown, a growth object is encapsulated in a vessel, and the growth object is grown without substantial influence of gravity.
摘要翻译： 为了生长待栽培或生长的对象，将生长对象包封在容器中，并且生长对象在不受重力的实质影响的情况下生长。
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公开(公告)号：US07413901B2
公开(公告)日：2008-08-19
申请号：US10258712
申请日：2001-03-13
申请人： Makoto Asashima , Naomi Moriya
发明人： Makoto Asashima , Naomi Moriya
CPC分类号： C12N5/0676 , A61K35/12 , C12N2501/385
摘要： The present invention provides a pancreas induced in vitro that is useful for developmental engineering and organ engineering, and a method wherein a pancreas induced in vitro which contributes to the development of diagnosis and treatment of pancreatic disorders for higher animals, can artificially and efficiently be induced from a gastrula apart from the presumptive region of pancreas. An explant which has a secretory gland-like structure wherein several cells are gathered and which expresses insulin is formed in vitro by treating the blastopore upper lip of an early gastrula of a vertebrate such as Xenopus with retinoic acid in vitro, and then culturing. The treatment with retinoic acid can be carried out, for example, by treating with retinoic acid at a concentration of 10−5 M or above for three hours.
摘要翻译： 本发明提供了可用于发育工程和器官工程的体外诱导的胰腺，以及其中可以人造和有效地诱导有助于发展诊断和治疗高等动物胰腺疾病的体外胰腺的方法 从胰腺的推定区域除外。 具有分泌腺样结构的外植体，其中几个细胞被收集并且表达胰岛素是通过体外通过用视黄酸处理诸如爪蟾的脊椎动物的早期胃肠的胚泡上唇，然后培养而形成的。 用视黄酸处理可以例如通过用浓度为10 -5 M以上的视黄酸处理3小时来进行。
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公开(公告)号：US20070161105A1
公开(公告)日：2007-07-12
申请号：US10549816
申请日：2004-03-17
CPC分类号： C12N5/0062 , C12N2501/385 , C12N2501/999 , C12N2506/00
摘要： A method for forming an organ and/or tissue from undifferentiated cells derived from a vertebrate animal in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid X receptor ligand (e.g., a retinoic acid X receptor agonist or antagonist), and a method for forming a pancreas from undifferentiated cells derived from a vertebrate animal in vitro or a method for forming a tissue having morphology and function of a pancreas from undifferentiated cells derived from a vertebrate in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid receptor ligand, together with activin, that does not substantially bind to the retinoic acid receptor subtype γ.
摘要翻译： 一种用于从体外衍生自脊椎动物的未分化细胞形成器官和/或组织的方法，其包括在视黄酸X受体配体（例如，视黄酸）存在下培养源自脊椎动物的未分化细胞的步骤 酸X受体激动剂或拮抗剂）以及从体外衍生自脊椎动物的未分化细胞形成胰腺的方法或用于从体外衍生自脊椎动物的未分化细胞形成具有胰腺形态和功能的组织的方法，其中 包括在视黄酸受体配体存在下培养来源于脊椎动物的未分化细胞以及基本上不与维甲酸受体亚型γ结合的激活素的步骤。
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公开(公告)号：US08105833B2
公开(公告)日：2012-01-31
申请号：US12535576
申请日：2009-08-04
CPC分类号： C12N5/0062 , C12N2501/385 , C12N2501/999 , C12N2506/00
摘要： A method for forming an organ and/or tissue from undifferentiated cells derived from a vertebrate animal in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid X receptor ligand (e.g., a retinoic acid X receptor agonist or antagonist), and a method for forming a pancreas from undifferentiated cells derived from a vertebrate animal in vitro or a method for forming a tissue having morphology and function of a pancreas from undifferentiated cells derived from a vertebrate in vitro, which comprises the step of culturing the undifferentiated cells derived from a vertebrate animal in the presence of a retinoic acid receptor ligand, together with activin, that does not substantially bind to the retinoic acid receptor subtype γ.
摘要翻译： 一种用于从体外衍生自脊椎动物的未分化细胞形成器官和/或组织的方法，其包括在视黄酸X受体配体（例如视黄酸）存在下培养源自脊椎动物的未分化细胞的步骤 酸X受体激动剂或拮抗剂）以及从体外衍生自脊椎动物的未分化细胞形成胰腺的方法或用于从体外衍生自脊椎动物的未分化细胞形成具有胰腺形态和功能的组织的方法，其中 包括在视黄酸受体配体存在下培养源自脊椎动物的未分化细胞与活化素一起基本上不结合视黄酸受体亚型γ的步骤。
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公开(公告)号：US07291500B2
公开(公告)日：2007-11-06
申请号：US11131241
申请日：2005-05-18
IPC分类号： C12N5/02
CPC分类号： C12M27/10 , C12M35/04 , C12N5/0062
摘要： In order to grow an object to be cultivated or grown, a growth object is encapsulated in a vessel, and the growth object is grown without substantial influence of gravity.
摘要翻译： 为了生长待栽培或生长的对象，将生长对象包封在容器中，并且生长对象在不受重力的实质影响的情况下生长。
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公开(公告)号：US08158423B2
公开(公告)日：2012-04-17
申请号：US12525983
申请日：2008-02-05
申请人： Hidehiro Ozawa , Mariko Yamaki , Makoto Asashima , Satoshi Ebina
发明人： Hidehiro Ozawa , Mariko Yamaki , Makoto Asashima , Satoshi Ebina
IPC分类号： C12N15/02
CPC分类号： G01N33/5082 , C12N5/0627 , C12N2501/105 , C12N2501/115 , C12N2501/15 , C12N2506/02 , C12N2506/1361 , C12N2533/54
摘要： A method of making a hair, including a step of culturing an undifferentiated cell of a mammal to produce an embryoid body and a step of further culturing the embryoid body is provided, wherein the culturing step is to culture the embryoid body on a three-dimensional matrix for 5 to 12 days. Furthermore, a biological material obtainable by the method of making a hair as described above is provided. Moreover, a biological material for a screening system of evaluating a medical product or the like, obtainable by utilizing the method of making a hair as described above is provided.
摘要翻译： 提供了一种制备头发的方法，包括培养哺乳动物的未分化细胞以产生胚状体的步骤和进一步培养胚状体的步骤，其中培养步骤是将胚状体在三维培养 基质5至12天。 此外，提供了可通过如上所述制造头发的方法获得的生物材料。 此外，提供了通过利用上述制造头发的方法获得的用于评估医疗产品等的筛选系统的生物材料。
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公开(公告)号：US07923245B2
公开(公告)日：2011-04-12
申请号：US10584371
申请日：2004-12-27
申请人： Miho Furue , Tetsuji Okamoto , Makoto Asashima
发明人： Miho Furue , Tetsuji Okamoto , Makoto Asashima
CPC分类号： C12N5/0606 , C12N5/0037 , C12N2500/90
摘要： The present invention discloses a medium for a serum-free medium capable of culturing ES cells for a long period while maintaining their undifferentiated state without using feeder cells, and a basal medium for producing the medium thus described. The basal medium of the present invention is characterized by that it has composition shown by Table I. Further, the present invention discloses a medium for ES cells produced with the basal medium.
摘要翻译： 本发明公开了一种能够长时间培养ES细胞，同时保持其未分化状态而不使用饲养细胞的无血清培养基，以及用于生产如此描述的培养基的基础培养基。 本发明的基础培养基的特征在于其具有表I所示的组成。此外，本发明公开了一种用基础培养基生产的ES细胞培养基。
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8.发明申请Application apparatus of 3-dimensional klinostat and method of growing object using the same 有权三维klinostat的应用装置和使用它的生长对象的方法公开(公告)号：US20050208650A1
公开(公告)日：2005-09-22
申请号：US11131241
申请日：2005-05-18
CPC分类号： C12M27/10 , C12M35/04 , C12N5/0062
摘要： In order to grow an object to be cultivated or grown, a growth object is encapsulated in a vessel, and the growth object is grown without substantial influence of gravity.
摘要翻译： 为了生长待栽培或生长的对象，将生长对象包封在容器中，并且生长对象在不受重力的实质影响的情况下生长。
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公开(公告)号：US20100304479A1
公开(公告)日：2010-12-02
申请号：US12525983
申请日：2008-02-05
申请人： Hidehiro Ozawa , Mariko Yamaki , Makoto Asashima , Satoshi Ebina
发明人： Hidehiro Ozawa , Mariko Yamaki , Makoto Asashima , Satoshi Ebina
IPC分类号： C12N5/0735
CPC分类号： G01N33/5082 , C12N5/0627 , C12N2501/105 , C12N2501/115 , C12N2501/15 , C12N2506/02 , C12N2506/1361 , C12N2533/54
摘要： A method of making a hair, including a step of culturing an undifferentiated cell of a mammal to produce an embryoid body and a step of further culturing the embryoid body is provided, wherein the culturing step is to culture the embryoid body on a three-dimensional matrix for 5 to 12 days. Furthermore, a biological material obtainable by the method of making a hair as described above is provided. Moreover, a biological material for a screening system of evaluating a medical product or the like, obtainable by utilizing the method of making a hair as described above is provided.
摘要翻译： 提供了一种制备头发的方法，包括培养哺乳动物的未分化细胞以产生胚状体的步骤和进一步培养胚状体的步骤，其中培养步骤是将胚状体在三维培养 基质5至12天。 此外，提供了可通过如上所述制造头发的方法获得的生物材料。 此外，提供了通过利用上述制造头发的方法获得的用于评估医疗产品等的筛选系统的生物材料。
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公开(公告)号：US20100015199A1
公开(公告)日：2010-01-21
申请号：US12439053
申请日：2007-08-27
申请人： Mariko Yamaki , Hidehiro Ozawa , Satoshi Ebina , Makoto Asashima
发明人： Mariko Yamaki , Hidehiro Ozawa , Satoshi Ebina , Makoto Asashima
CPC分类号： C12N5/0606 , A61L27/3834 , C12N5/0603 , C12N2533/54
摘要： The present invention provides a method for suppressing generation of a teratoma of an undifferentiated cell, preferably an ES cell. A biological material obtainable by the method and a therapeutic method for transplanting the biological material into a subject are also provided.An undifferentiated cell, in particular, preferably an ES cell is cultured to produce an increased embryoid body-like and the embryoid body-like is cultured for at least one week on, and adhered to, a three-dimensional solid type-I collagen carrier or an analogue thereof. Furthermore, a biological material is obtained thereby and the biological material is transplanted into a mammalian subject.
摘要翻译： 本发明提供抑制未分化细胞，优选ES细胞的畸胎瘤的产生的方法。 还提供了通过该方法获得的生物材料和用于将生物材料移植到受试者中的治疗方法。 培养未分化细胞，特别优选ES细胞以产生增加的类胚体，并将胚状体样培养至少一周，并附着于三维固体I型胶原载体上 或其类似物。 此外，由此获得生物材料，将生物材料移植到哺乳动物受试者中。
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