公开(公告)号：US06178382B1

公开(公告)日：2001-01-23

申请号：US09103284

申请日：1998-06-23

申请人： Mario Roederer ,  Adam S. Treister ,  Martin Bigos ,  David R. Parks ,  Wayne A. Moore ,  Leonore A. Herzenberg

发明人： Mario Roederer ,  Adam S. Treister ,  Martin Bigos ,  David R. Parks ,  Wayne A. Moore ,  Leonore A. Herzenberg

IPC分类号： G01N3350

CPC分类号： G06T11/206 ,  G01N33/50

摘要： This invention relates a series of methods for the visual representation and subsequent application of analyses on complex data sets. In particular, this invention is useful for the analysis of multiple sample sets that share common features, for which similar types of analyses are desired. Three concepts are embodied to aid in this analysis: “Functional equivalence by Algorithmic Polymorphism” (FEAP), in which an analysis algorithm is abstracted through the use of an associated name; a genealogical metaphor for the representation of successive or parallel analysis steps, in which “families” of analyses can be easily copied between different sample data sets; and batch analysis through the creation of multi-sample analysis surrogates (MSAS), which are groups of samples wherein analyses applied to an MSAS is then applied to every sample within the MSAS group. This invention has particular utility in the analysis of data derived from flow cytometers, the analysis of complex demographic data, and other similar data sets.

摘要翻译： 本发明涉及用于在复杂数据集上的分析的视觉表示和后续应用的一系列方法。 特别地，本发明对于分析共享共同特征的多个样本集是有用的，对于类似的分析类型是期望的。 三个概念被体现为帮助分析：“通过算法多态性的功能等同”（FEAP），其中分析算法通过使用关联名称被抽象; 用于表示连续或平行分析步骤的系谱比喻，其中分析的“家庭”可以容易地在不同样本数据集之间复制; 并通过创建多样本分析代理（MSAS）进行批量分析，这些代码是MSAS组中每个样本的样本组，其中分析应用于MSAS。 本发明在分析流式细胞仪获得的数据，复杂人口统计学数据分析和其他类似数据集方面具有特别的实用价值。

公开(公告)号：US06673556B2

公开(公告)日：2004-01-06

申请号：US09954392

申请日：2001-09-12

申请人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

发明人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

IPC分类号： G01N3353

CPC分类号： G01N33/5005 ,  G01N33/582 ,  G01N2500/10 ,  G01N2800/52

摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。

公开(公告)号：US07186516B2

公开(公告)日：2007-03-06

申请号：US10697737

申请日：2003-10-29

申请人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

发明人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

IPC分类号： G01N33/567 ,  C12Q1/00

CPC分类号： G01N33/5005 ,  G01N33/582 ,  G01N2500/10 ,  G01N2800/52

摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供了检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。

公开(公告)号：US20050079556A1

公开(公告)日：2005-04-14

申请号：US10697737

申请日：2003-10-29

申请人： Douglas Nixon ,  Adrian McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

发明人： Douglas Nixon ,  Adrian McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

IPC分类号： G01N33/50 ,  G01N33/58 ,  G01N33/567

CPC分类号： G01N33/5005 ,  G01N33/582 ,  G01N2500/10 ,  G01N2800/52

摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供了检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。

公开(公告)号：US20050223024A1

公开(公告)日：2005-10-06

申请号：US11096011

申请日：2005-03-30

申请人： William Hyun ,  Martin Bigos ,  Antony Chiang ,  Lawrence Roberts

发明人： William Hyun ,  Martin Bigos ,  Antony Chiang ,  Lawrence Roberts

IPC分类号： G06F17/00 ,  G06F17/30

CPC分类号： G06F16/168

摘要： Methods, systems and computer readable media for users of a shared database, file system, or other similar software system to browse files or records in the database according to any of the files' attributes in a standard hierarchical tree structure. Methods, systems and computer readable media that enable users to dynamically define and customize a hierarchy of files according to the quantity and priority of attributes that each user individually desires, such that the records or files are automatically rearranged and reorganized into the user-created hierarchy.

摘要翻译： 用于共享数据库，文件系统或其他类似软件系统的用户的方法，系统和计算机可读介质，以根据标准分层树结构中的任何文件属性浏览数据库中的文件或记录。 方法，系统和计算机可读介质，使用户能够根据每个用户单独期望的属性的数量和优先级动态地定义和定制文件的层次结构，使得记录或文件被自动重新排列并重新组织成用户创建的层次结构 。

公开(公告)号：US20050203976A1

公开(公告)日：2005-09-15

申请号：US11055424

申请日：2005-02-09

申请人： William Hyun ,  Martin Bigos ,  Antony Chiang ,  Lawrence Roberts

发明人： William Hyun ,  Martin Bigos ,  Antony Chiang ,  Lawrence Roberts

IPC分类号： G06F17/30

CPC分类号： G06F16/1844

摘要： Methods, tools and systems for efficiently and intuitively transferring files from client computers, via a network, to a centralized location for storage of the files as well as storage of metadata associated with the files in a manner such that the metadata as well as the files may be searched in the database by users to identify files of interest.

摘要翻译： 用于有效和直观地将文件从客户端计算机经由网络传输到用于存储文件的集中位置以及与文件相关联的元数据的存储的方法，工具和系统，使得元数据以及文件 可以在用户的​​数据库中搜索以识别感兴趣的文件。