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    Method of identifying and treating invasive carcinomas
    2.
    发明授权
    Method of identifying and treating invasive carcinomas 失效
    识别和治疗侵袭性癌的方法

    公开(公告)号：US06689614B1

    公开(公告)日：2004-02-10

    申请号：US10369032

    申请日：2003-02-18

    申请人： Karl X. Chai ,  Li-Mei Chen ,  Lee Chao ,  Julie Chao

    发明人： Karl X. Chai ,  Li-Mei Chen ,  Lee Chao ,  Julie Chao

    IPC分类号： G01N3350

    CPC分类号： G01N33/574 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/158

    摘要： Prostasin protein has been found to be a useful marker for determination of the invasiveness of and as a means to treat human carcinomas. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in normal human prostate epithelial cells and the human prostate cancer cell line LNCaP, but not in the highly invasive human prostate cancer cell lines DU-145 and PC-3. Imunohistochemistry studies of human prostate cancer specimens revealed a down-regulation of prostasin in high-grade tumors. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in a non-invasive human breast cancer cell line, MCF-7, while invasive human breast cancer cell lines MDA-MB-231 and MDA-MB-435s were found not to express either the prostasin protein or the mRNA. A non-invasive human breast cancer cell line, MDA-MB-453, was shown to express prostasin mRNA but not prostasin protein. Transfection of DU-145 and PC-3 cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 68% and 42%, respectively. Transfection of MDA-MB-231 and MDA-MB-435s cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 50% for either cell line.

    摘要翻译： 已经发现前列腺素蛋白是用于测定人类癌的侵袭性和作为手段治疗人类癌症的有用标志物。 使用RT-PCR和蛋白质印迹分析，在正常人前列腺上皮细胞和人前列腺癌细胞系LNCaP中发现前列腺素蛋白和mRNA表达，但不存在于高侵袭性人前列腺癌细胞系DU-145和PC-3中。 人类前列腺癌标本的免疫组织化学研究显示高级肿瘤中前列腺素的下调。 使用RT-PCR和蛋白质印迹分析，在非侵入性人乳腺癌细胞系MCF-7中发现前列腺素蛋白和mRNA表达，而侵袭性人乳腺癌细胞系MDA-MB-231和MDA-MB-435s分别为 发现不表达前列腺素蛋白或mRNA。 非侵入性人乳腺癌细胞系MDA-MB-453显示表达前列腺素mRNA而不是前列腺素蛋白。 DU-145和PC-3细胞用全长人前列腺素cDNA的转染能够恢复前列腺素的表达，体外侵袭能力分别降低了68％和42％。 用全长人前列腺素cDNA转染MDA-MB-231和MDA-MB-435s细胞恢复前列腺素的表达，并将任何细胞系的体外侵袭力降低50％。

    Method for diagnosing and treating chronic pelvic pain syndrome
    4.
    发明授权
    Method for diagnosing and treating chronic pelvic pain syndrome 失效
    诊断和治疗慢性盆腔疼痛综合征的方法

    公开(公告)号：US06180355B2

    公开(公告)日：2001-01-30

    申请号：US09306927

    申请日：1999-05-07

    申请人： Richard B. Alexander ,  Sathibalan Ponniah

    发明人： Richard B. Alexander ,  Sathibalan Ponniah

    IPC分类号： G01N3350

    CPC分类号： G01N33/6863 ,  A61K38/1793

    摘要： The present invention provides a superior method of diagnosing Chronic Pelvic Pain Syndrome in men comprising measuring levels of cytokines in semen or components or fractions of semen. The invention also provides a method of treating a condition associated with elevated levels of a cytokine, such as TNF-&agr;, in semen or a component or fraction thereof, comprising administering a therapeutically effective amount of an ant-cytokine compound or composition, such as an anti-TNF-&agr; compound or composition.

    摘要翻译： 本发明提供了一种诊断男性慢性骨盆疼痛综合征的优良方法，包括测量精液中精液或成分或精馏分数的细胞因子水平。 本发明还提供了一种治疗与精液或其组分或部分中的细胞因子如TNF-α水平升高相关的病症的方法，包括施用治疗有效量的抗细胞因子化合物或组合物，例如 抗TNF-α化合物或组合物。

    Optoelectronic sensor
    6.
    发明授权
    Optoelectronic sensor 有权
    光电传感器

    公开(公告)号：US06800448B2

    公开(公告)日：2004-10-05

    申请号：US09848811

    申请日：2001-05-04

    申请人： Todd H. Rider ,  Laura Smith

    发明人： Todd H. Rider ,  Laura Smith

    IPC分类号： G01N3350

    CPC分类号： G01N21/6454 ,  G01N21/76 ,  G01N21/77 ,  G01N33/54366 ,  G01N33/554 ,  G01N33/56972 ,  G01N33/582 ,  G01N2021/6482 ,  G01N2021/7786 ,  Y10S435/808 ,  Y10S436/805

    摘要： A device for detecting the presence of an antigen including (1) a cell having antibodies which are expressed on the surface of the cell and are specific for the antigen to be detected, where binding of the antigen to the antibodies results in an increase in calcium concentration in the cytosol of the cell, the cell further having a emitter molecule which, in response to the increased calcium concentration in the cytosol, emits a photon; (2) a liquid medium for receiving the antigen and in which the cell is immersed; and (3) an optical detector arranged for receiving the photon emitted from the cell.

    摘要翻译： 用于检测抗原存在的装置，包括（1）具有在细胞表面上表达且对待检测抗原特异的抗体的细胞，其中抗原与抗体的结合导致钙的增加 浓度在细胞的细胞溶质中，细胞还具有发射分子，其响应于细胞质中钙浓度的增加而发射光子; （2）用于接收抗原的液体培养基，其中浸泡细胞; 和（3）布置成用于接收从该单元发射的光子的光学检测器。

    Methods of measuring tissue factor pathway inhibitor-3
    8.
    发明授权
    Methods of measuring tissue factor pathway inhibitor-3 失效
    测量组织因子途径抑制剂的方法3

    公开(公告)号：US06548262B2

    公开(公告)日：2003-04-15

    申请号：US09827948

    申请日：2001-04-06

    申请人： Reiner L. Gentz ,  Tsu-An Hsu ,  Craig A. Rosen ,  Jian Ni

    发明人： Reiner L. Gentz ,  Tsu-An Hsu ,  Craig A. Rosen ,  Jian Ni

    IPC分类号： G01N3350

    CPC分类号： C07K14/8114 ,  A61K38/00 ,  C07K2319/00

    摘要： The present invention relates to a novel TFPI-3 protein which is a member of the tissue factor protease inhibitor family. In particular, isolated nucleic acid molecules are provided encoding human TFPI-3 proteins. TFPI-3 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of TFPI-3 activity. Also provided are diagnostic methods for detecting hemostasis system-related disorders and therapeutic methods for treating hemostatis system-related disorders.

    摘要翻译： 本发明涉及作为组织因子蛋白酶抑制剂家族成员的新型TFPI-3蛋白。 特别地，提供编码人TFPI-3蛋白质的分离的核酸分子。 还提供TFPI-3多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定TFPI-3活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测止血系统相关疾病的诊断方法和治疗止血系统相关疾病的治疗方法。

    Antibodies that specifically bind Cytostatin III
    9.
    发明授权
    Antibodies that specifically bind Cytostatin III 失效
    特异性结合细胞抑素III的抗体

    公开(公告)号：US06413726B1

    公开(公告)日：2002-07-02

    申请号：US09734036

    申请日：2000-12-12

    申请人： Jian Ni ,  Guo-Liang Yu ,  Reiner Gentz ,  Patrick J. Dillon

    发明人： Jian Ni ,  Guo-Liang Yu ,  Reiner Gentz ,  Patrick J. Dillon

    IPC分类号： G01N3350

    CPC分类号： C07K14/475 ,  C07K2319/00

    摘要： The invention relates to Cytostatin III polypeptides, polynucleotides encoding the polypeptides, methods for producing the polypeptides, in particular by expressing the polynucleotides, and agonists and antagonists of the polypeptides. The invention further relates to methods for utilizing such polynucleotides, polypeptides, agonists and antagonists for applications, which relate, in part, to research, diagnostic and clinical arts.

    摘要翻译： 本发明涉及细胞抑制素III多肽，编码多肽的多核苷酸，产生多肽的方法，特别是通过表达多核苷酸，多肽的激动剂和拮抗剂。 本发明还涉及用于应用的这些多核苷酸，多肽，激动剂和拮抗剂的方法，其部分涉及研究，诊断和临床领域。

    Methods for analysis of large sets of multiparameter data
    10.
    发明授权
    Methods for analysis of large sets of multiparameter data 失效
    大量多参数数据分析方法

    公开(公告)号：US06178382B1

    公开(公告)日：2001-01-23

    申请号：US09103284

    申请日：1998-06-23

    申请人： Mario Roederer ,  Adam S. Treister ,  Martin Bigos ,  David R. Parks ,  Wayne A. Moore ,  Leonore A. Herzenberg

    发明人： Mario Roederer ,  Adam S. Treister ,  Martin Bigos ,  David R. Parks ,  Wayne A. Moore ,  Leonore A. Herzenberg

    IPC分类号： G01N3350

    CPC分类号： G06T11/206 ,  G01N33/50

    摘要： This invention relates a series of methods for the visual representation and subsequent application of analyses on complex data sets. In particular, this invention is useful for the analysis of multiple sample sets that share common features, for which similar types of analyses are desired. Three concepts are embodied to aid in this analysis: “Functional equivalence by Algorithmic Polymorphism” (FEAP), in which an analysis algorithm is abstracted through the use of an associated name; a genealogical metaphor for the representation of successive or parallel analysis steps, in which “families” of analyses can be easily copied between different sample data sets; and batch analysis through the creation of multi-sample analysis surrogates (MSAS), which are groups of samples wherein analyses applied to an MSAS is then applied to every sample within the MSAS group. This invention has particular utility in the analysis of data derived from flow cytometers, the analysis of complex demographic data, and other similar data sets.

    摘要翻译： 本发明涉及用于在复杂数据集上的分析的视觉表示和后续应用的一系列方法。 特别地，本发明对于分析共享共同特征的多个样本集是有用的，对于类似的分析类型是期望的。 三个概念被体现为帮助分析：“通过算法多态性的功能等同”（FEAP），其中分析算法通过使用关联名称被抽象; 用于表示连续或平行分析步骤的系谱比喻，其中分析的“家庭”可以容易地在不同样本数据集之间复制; 并通过创建多样本分析代理（MSAS）进行批量分析，这些代码是MSAS组中每个样本的样本组，其中分析应用于MSAS。 本发明在分析流式细胞仪获得的数据，复杂人口统计学数据分析和其他类似数据集方面具有特别的实用价值。