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公开(公告)号：US20130180007A1
公开(公告)日：2013-07-11
申请号：US13445391
申请日：2012-04-12
申请人： Mark A. Chamberlin , Shai J. Lawit
发明人： Mark A. Chamberlin , Shai J. Lawit
IPC分类号： C12N15/82
CPC分类号： C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12N15/8278 , C12N15/8279 , C12N15/8281 , C12N15/8282 , C12N15/8283 , C12N15/8286 , C12N15/8287 , C12N15/8289 , Y02A40/146
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for several Arabidopsis thaliana ovule somatic tissue-preferred promoters AT-CYP86C1, AT-PPM, AT-EXT, AT-GILT1 and AT-TT2. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译： 提供了用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括几个拟南芥胚珠体细胞组织优选启动子AT-CYP86C1，AT-PPM，AT-EXT，AT-GILT1和AT-TT2的核苷酸序列。 还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。
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2.发明申请公开(公告)号：US20130180010A1
公开(公告)日：2013-07-11
申请号：US13445447
申请日：2012-04-12
申请人： Mark A. Chamberlin , Shai J. Lawit
发明人： Mark A. Chamberlin , Shai J. Lawit
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8261 , C12N15/8287
摘要： Methods and compositions are provided to increase the activity/level of an RKD polypeptide or an active variant or fragment thereof in an unreduced ovule plant cell that is outside of the embryo sac. In specific embodiments, such modulation of activity/level of the RKD polypeptide promotes an egg cell-like state in an unreduced ovule plant cell that is outside of the embryo sac. Such methods and compositions can employ an expression construct comprising a RKD polypeptide or active variant or fragment thereof operably linked to an ovule tissue-preferred promoter, in particular an ovule tissue-preferred promoter that is active in at least one non-gametophyte tissue in a plant ovule and is active in an unreduced cell that is outside of the embryo sac.
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公开(公告)号：US20070271629A1
公开(公告)日：2007-11-22
申请号：US11804325
申请日：2007-05-17
申请人： Evgueni Ananiev , Mark A. Chamberlin , William J. Gordon-Kamm , Sergei Svitashev , Chengcang Wu
发明人： Evgueni Ananiev , Mark A. Chamberlin , William J. Gordon-Kamm , Sergei Svitashev , Chengcang Wu
CPC分类号： C12N15/82 , C12N15/8201
摘要： Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
摘要翻译： 描述了包含特异性结合着丝粒蛋白C（CENPC）的功能着丝粒的人造植物微染色体和制备这种微染色体的方法。
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公开(公告)号：US20090100550A1
公开(公告)日：2009-04-16
申请号：US12142953
申请日：2008-06-20
申请人： Evgueni Ananiev , Mark A. Chamberlin , William J. Gordon-Kamm , Sergei Svitashev , Chengcang Wu
发明人： Evgueni Ananiev , Mark A. Chamberlin , William J. Gordon-Kamm , Sergei Svitashev , Chengcang Wu
CPC分类号： C12N15/8201 , C12N15/82
摘要： Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
摘要翻译： 描述了包含特异性结合着丝粒蛋白C（CENPC）的功能着丝粒的人造植物微染色体和制备这种微染色体的方法。
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公开(公告)号：US06905857B2
公开(公告)日：2005-06-14
申请号：US10246581
申请日：2002-09-18
申请人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
发明人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
CPC分类号： C12N9/00 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.
摘要翻译： 提供了编码在玉米植物中活性的两种RAD51重组酶的核酸序列。 还提供了包括可用作RFLP探针的ZmRAD51编码序列和独特的3'-非翻译区的cDNA序列。 还公开了含有编码ZmRAD51融合蛋白的核酸序列的质粒的产生以及使用质粒将ZmRAD51编码序列引入宿主细胞如玉米细胞的方法。
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公开(公告)号：US06541684B1
公开(公告)日：2003-04-01
申请号：US09246963
申请日：1999-02-09
申请人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
发明人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
IPC分类号： A01H500
CPC分类号： C12N9/00 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.
摘要翻译： 提供了编码在玉米植物中活性的两种RAD51重组酶的核酸序列。 还提供了包括可用作RFLP探针的ZmRAD51编码序列和独特的3'-非翻译区的cDNA序列。 还公开了含有编码ZmRAD51融合蛋白的核酸序列的质粒的产生以及使用质粒将ZmRAD51编码序列引入宿主细胞如玉米细胞的方法。
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公开(公告)号：US20090165176A1
公开(公告)日：2009-06-25
申请号：US12181380
申请日：2008-07-29
申请人： Evgueni Ananiev , Olga N. Danilevskaya , Mark A. Chamberlin , William J. Gordon-Kamm , Sergei Svitashev , Chengcang C. Wu
发明人： Evgueni Ananiev , Olga N. Danilevskaya , Mark A. Chamberlin , William J. Gordon-Kamm , Sergei Svitashev , Chengcang C. Wu
CPC分类号： C12N15/82 , C12N15/8201
摘要： Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
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