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公开(公告)号：US20080066549A1
公开(公告)日：2008-03-20
申请号：US11857419
申请日：2007-09-18
IPC分类号： G01N33/00
CPC分类号： G01N21/648 , G01N2021/6432
摘要： An embodiment relates generally to a method for analysis of a nucleic acid. The method includes providing for a resonant structure configured to couple with one or more fluorescently labeled nucleic acids and directing an excitation light from a source on the resonant structure. The method also includes generating plasmons on the surface of the resonant structure where the analyte is fixed at a point of energy concentration of the resonant structure.
摘要翻译： 实施方案一般涉及核酸分析方法。 该方法包括提供构造成与一个或多个荧光标记的核酸耦合并将来自源的激发光引导到谐振结构上的谐振结构。 该方法还包括在谐振结构的表面上产生等离子体激元，其中分析物固定在谐振结构的能量集中点处。
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公开(公告)号：US20090140128A1
公开(公告)日：2009-06-04
申请号：US12284109
申请日：2008-09-18
申请人： Mark F. Oldham , Eric S. Nordman , Charles R. Connell , Sun Hongye , Steven M. Blair , Christina E. Inman
发明人： Mark F. Oldham , Eric S. Nordman , Charles R. Connell , Sun Hongye , Steven M. Blair , Christina E. Inman
IPC分类号： G12B21/06
CPC分类号： G01N21/648 , G01N2021/6432
摘要： An embodiment relates generally to resonant structure. The resonant structure includes a substrate and a nano-bowtie antenna deposited over the substrate. The resonant structure also includes an enclosure deposited over the substrate and surrounding the nano-bowtie antenna, where the enclosure is configured to raise an enhancement level in the nano-bowtie antenna.
摘要翻译： 实施例一般涉及谐振结构。 谐振结构包括沉积在衬底上的衬底和纳米蝴蝶结天线。 谐振结构还包括沉积在衬底上并围绕纳米天线的外壳，其中外壳被配置为提高纳米天线的增强水平。
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3.发明申请Compositions and Methods for Clonal Amplification and Analysis of Polynucleotides 审中-公开多核苷酸克隆扩增和分析的组合物和方法公开(公告)号：US20090325184A1
公开(公告)日：2009-12-31
申请号：US12555730
申请日：2009-09-08
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12Q1/6844 , C12Q2565/501 , C12Q2537/143 , C12Q2527/125
摘要： Compositions and methods of use are disclosed for clonally amplifying and analyzing one or more polynucleotides.
摘要翻译： 公开了用于克隆扩增和分析一种或多种多核苷酸的组合物和使用方法。
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公开(公告)号：US20100103416A1
公开(公告)日：2010-04-29
申请号：US12630683
申请日：2009-12-03
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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公开(公告)号：US07636160B2
公开(公告)日：2009-12-22
申请号：US12321781
申请日：2009-01-26
申请人： Mark F. Oldham , Eric S. Nordman , Richard T. Reel , John S. Shigeura , Janice G. Shigeura, legal representative
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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公开(公告)号：US09410889B2
公开(公告)日：2016-08-09
申请号：US11423403
申请日：2006-06-09
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte.
摘要翻译： 本公开提供了用于单独和基本同时地检测来自多个局部发光分析物的光发射的装置，系统和方法。 根据本公开的示例性实施例的系统包括具有形成在其上的结构的样本保持器，用于空间分离和约束各自具有单个核酸分子或单个核酸聚合酶的多个发光分析物，光源被配置为 照亮样品架，光学组件被配置成分别收集和检测与多个发光分析物相关联的基本上同时的发光。 该系统还可以包括被配置为分析光发射以确定与每个分析物相关联的靶核酸分子的结构或性质的计算机系统。
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公开(公告)号：US07636159B2
公开(公告)日：2009-12-22
申请号：US12283957
申请日：2008-09-17
申请人： Mark F. Oldham , Eric S. Nordman , Richard T. Reel , John S. Shigeura , Janice G. Shigeura, legal representative
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
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公开(公告)号：US20090305287A1
公开(公告)日：2009-12-10
申请号：US12482795
申请日：2009-06-11
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： A method for identifying nucleotides in a nucleic acid sequence is disclosed. A plurality of polymerization complexes are provided within a plurality of confined reaction environments. Each complex comprises a polymerase enzyme and a template nucleic acid. The plurality of complexes are contacted with a plurality of types of nucleotide analogs labeled with distinguishable fluorescent labels under conditions suitable for polymerization. Fluorescent signals associated with incorporation of a nucleotide analog are transmitted to a detector, wherein the location of the fluorescent signal on the detector is indicative of the individual confined reaction environment and the type of nucleotide incorporated. The nucleotide in a nucleic acid sequence is identified based upon the type of nucleotide incorporated and the confined reaction environment.
摘要翻译： 公开了鉴定核酸序列中的核苷酸的方法。 在多个限制反应环境中提供多个聚合复合物。 每个复合物包含聚合酶和模板核酸。 在适于聚合的条件下，多种复合物与多种类型的可区分荧光标记的核苷酸类似物接触。 与核苷酸类似物掺入相关的荧光信号被传送到检测器，其中荧光信号在检测器上的位置指示个体限制的反应环境和掺入的核苷酸的类型。 核酸序列中的核苷酸是根据所掺入的核苷酸类型和限制性反应环境来鉴定的。
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公开(公告)号：US20090250615A1
公开(公告)日：2009-10-08
申请号：US12384516
申请日：2009-04-06
申请人： Mark F. Oldham , Eric S. Nordman , Hongye Sun , Steven J. Boege
发明人： Mark F. Oldham , Eric S. Nordman , Hongye Sun , Steven J. Boege
IPC分类号： G01T1/20
CPC分类号： G01N21/6486 , G01N21/6428 , G01N21/6452 , G01N21/6456
摘要： A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.
摘要翻译： 提供了一种扫描检测系统，其中检测到来自流动池中的位置的发射。 在一些实施例中，系统可以包括激发源，光切割源和调制光学器件，其被配置为使得由激发源产生的激发光束照射第一组固定位置并引起由光切割源产生的光致剪切光束 以照射与第一组固定位置分开并分开的第二组固定位置。 还提供了使用这种系统检测测序反应的方法。
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公开(公告)号：US20090021735A1
公开(公告)日：2009-01-22
申请号：US12283957
申请日：2008-09-17
IPC分类号： G01N21/62
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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