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公开(公告)号：US20090305908A1
公开(公告)日：2009-12-10
申请号：US12482767
申请日：2009-06-11
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： A method for preferentially localizing desired molecules within an optical confinement disposed upon a substrate is disclosed. The desired molecules are deposited over the surface of the substrate. The desired molecules that are not within the optical confinement are selectively removed from the surface of the substrate.
摘要翻译： 公开了一种用于在设置在基板上的光限制内优先定位所需分子的方法。 期望的分子沉积在衬底的表面上。 不在光学限制范围内的期望分子被选择性地从衬底的表面去除。
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公开(公告)号：US09410889B2
公开(公告)日：2016-08-09
申请号：US11423403
申请日：2006-06-09
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte.
摘要翻译： 本公开提供了用于单独和基本同时地检测来自多个局部发光分析物的光发射的装置，系统和方法。 根据本公开的示例性实施例的系统包括具有形成在其上的结构的样本保持器，用于空间分离和约束各自具有单个核酸分子或单个核酸聚合酶的多个发光分析物，光源被配置为 照亮样品架，光学组件被配置成分别收集和检测与多个发光分析物相关联的基本上同时的发光。 该系统还可以包括被配置为分析光发射以确定与每个分析物相关联的靶核酸分子的结构或性质的计算机系统。
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公开(公告)号：US20090305287A1
公开(公告)日：2009-12-10
申请号：US12482795
申请日：2009-06-11
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： A method for identifying nucleotides in a nucleic acid sequence is disclosed. A plurality of polymerization complexes are provided within a plurality of confined reaction environments. Each complex comprises a polymerase enzyme and a template nucleic acid. The plurality of complexes are contacted with a plurality of types of nucleotide analogs labeled with distinguishable fluorescent labels under conditions suitable for polymerization. Fluorescent signals associated with incorporation of a nucleotide analog are transmitted to a detector, wherein the location of the fluorescent signal on the detector is indicative of the individual confined reaction environment and the type of nucleotide incorporated. The nucleotide in a nucleic acid sequence is identified based upon the type of nucleotide incorporated and the confined reaction environment.
摘要翻译： 公开了鉴定核酸序列中的核苷酸的方法。 在多个限制反应环境中提供多个聚合复合物。 每个复合物包含聚合酶和模板核酸。 在适于聚合的条件下，多种复合物与多种类型的可区分荧光标记的核苷酸类似物接触。 与核苷酸类似物掺入相关的荧光信号被传送到检测器，其中荧光信号在检测器上的位置指示个体限制的反应环境和掺入的核苷酸的类型。 核酸序列中的核苷酸是根据所掺入的核苷酸类型和限制性反应环境来鉴定的。
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公开(公告)号：US20100261158A1
公开(公告)日：2010-10-14
申请号：US11423403
申请日：2006-06-09
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte.
摘要翻译： 本公开提供了用于单独和基本同时地检测来自多个局部发光分析物的光发射的装置，系统和方法。 根据本公开的示例性实施例的系统包括具有形成在其上的结构的样本保持器，用于空间分离和约束各自具有单个核酸分子或单个核酸聚合酶的多个发光分析物，光源被配置为 照亮样品架，光学组件被配置成分别收集和检测与多个发光分析物相关联的基本上同时的发光。 该系统还可以包括被配置为分析光发射以确定与每个分析物相关联的靶核酸分子的结构或性质的计算机系统。
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公开(公告)号：US20090305909A1
公开(公告)日：2009-12-10
申请号：US12482815
申请日：2009-06-11
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： A method for selectively immobilizing a molecule of interest on a substrate is disclosed. A substrate having a first surface component and a second surface component is provided. The first and second surface components have different surface characteristics. The molecule of interest is selectively coupled to the first surface component based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component.
摘要翻译： 公开了一种用于选择性地将感兴趣的分子固定在基底上的方法。 提供了具有第一表面部件和第二表面部件的基板。 第一和第二表面部件具有不同的表面特性。 基于第一表面成分的表面特性和第二表面成分的表面特性之间的差异，感兴趣的分子选择性地耦合到第一表面成分。
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公开(公告)号：US07636160B2
公开(公告)日：2009-12-22
申请号：US12321781
申请日：2009-01-26
申请人： Mark F. Oldham , Eric S. Nordman , Richard T. Reel , John S. Shigeura , Janice G. Shigeura, legal representative
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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公开(公告)号：US09926596B2
公开(公告)日：2018-03-27
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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8.发明授权公开(公告)号：US09187783B2
公开(公告)日：2015-11-17
申请号：US13824129
申请日：2011-10-04
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.
摘要翻译： 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。
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公开(公告)号：US09151751B2
公开(公告)日：2015-10-06
申请号：US12165379
申请日：2008-06-30
申请人： Mark F. Oldham , Eric S. Nordman
发明人： Mark F. Oldham , Eric S. Nordman
IPC分类号： G01N33/543 , C12Q1/68 , G01N27/414
CPC分类号： G01N33/54373 , B82Y15/00 , C12Q1/6825 , G01N27/4145 , G01N27/4146 , C12Q2563/155 , C12Q2563/113
摘要： There is disclosed a system for electrical charge detection comprising a nanoFET device. Also disclosed is a method of electrical charge detection for single molecule sequencing. The method includes attaching a macromolecule or assemblies thereof to a gate of a nanoFET device and flowing in a solution of charge tags, where a charge tag includes a nucleotide attached to a charge complex. The method also includes incorporating one charge tag into the macromolecule or assemblies thereof and cleaving the charge tags from the macromolecule or assemblies thereof. The method further includes detecting at least one of current and voltage from the nanoFET device.
摘要翻译： 公开了一种包括纳米器件的电荷检测系统。 还公开了单分子测序的电荷检测方法。 该方法包括将大分子或其组合物连接到纳米器件的栅极并在电荷标签的溶液中流动，其中电荷标签包含附着于电荷复合物的核苷酸。 该方法还包括将一个电荷标签掺入大分子或其组件中，并从大分子或其组件切割电荷标记。 该方法还包括从nanoFET器件检测电流和电压中的至少一个。
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公开(公告)号：US20140235457A1
公开(公告)日：2014-08-21
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parazi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parazi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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