摘要:
Analytes and ligands for detecting animal byproduct in agricultural samples such as animal feed to minimize the transmission of prion pathogens into food products consumed by humans. The analyte is a component of rendered animal byproduct such as meat and bone meal (MBM). An antibody specific for the analyte is produced by immunizing an animal with MBM or an immunogenic component of MBM that is found in rendered animal product.
摘要:
Reagents, immunoassay methods, and kits for detecting the presence or amount of trichloroethylene or perchloroethylene in a sample. Reagents include antibodies, immunogens for preparing antibodies, and assay conjugates. The immunogens elicit high affinity antibodies to trichloroethylene. The antibodies have minimal crossreactivity to other halogenated and aromatic hydrocarbons. The assay conjugates provide for sufficient binding of the antibody to the conjugate with maximum inhibition of that binding by free trichloroethylene and perchloroethylene in the sample. The reagents are useful in immunoassays to detect trichloroethylene and perchloroethylene, especially in environmental samples. Kits contain antibodies specific for trichloroethylene and perchloroethylene and assay conjugates.
摘要:
Compositions containing bacteriophages and methods of using bacteriophages in microorganism detection assays and microbial growth and plating media are disclosed. The lytic ability of these phages to control the growth of non-target populations provides superior sensitivity and specificity to detection assays and reduces false negative and false positive results. The removal of contaminating bacteria reduces the microbial competition for nutrients in the growth media thereby increasing the efficiency and productivity of the culture. The phage treatment of the sample increases the proportion of target microorganisms in the sample over contaminating bacteria thereby requiring less time for enrichment to obtain a significant signal improving overall signal to noise ratio in assays and providing for higher yield of end product in microbiological production systems.
摘要:
The invention provides an antibody having an affinity to water treatment polymers. The antibodies of the invention are used in assays to determine the presence or concentration of a water treatment polymer in a fluid or aqueous system.
摘要:
Methods are provided for preparing patient samples for direct detection and identification of microorganisms in the patient samples using multiplex immunoassays. The method includes enzymatic treatment of the patient sample, boiling and centrifugation of the sample to provide a treated sample that may be used in an immunoassay. There are also provided immunoassay compositions and systems for the direct detection and identification of microorganism in patient tissue.
摘要:
Compositions containing bacteriophages and methods of using bacteriophages in microorganism detection assays and microbial growth and plating media are disclosed. The lytic ability of these phages to control the growth of non-target populations provides superior sensitivity and specificity to detection assays and reduces false negative and false positive results. The removal of contaminating bacteria reduces the microbial competition for nutrients in the growth media thereby increasing the efficiency and productivity of the culture. The phage treatment of the sample increases the proportion of target microorganisms in the sample over contaminating bacteria thereby requiring less time for enrichment to obtain a significant signal improving overall signal to noise ratio in assays and providing for higher yield of end product in microbiological production systems.
摘要:
Compositions and methods utilizing Raman Spectroscopy to detect and determine, directly or indirectly, the concentration of water treatment polymers in water or methods for measuring or quantifying the amount of water treatment polymer in a sample, such as an industrial, commercial, or municipal water sample, are provided. The compositions contain water treatment polymers that have been modified to include one or more Raman-active functional groups. The methods utilize Raman spectroscopy to detect the presence or amount of water treatment polymer either directly or indirectly. The method provides the ability to detect or measure the amount of active water treatment polymer remaining in the sample.
摘要:
An assay device for the detection of analyte in a sample, methods and immunoassay formats for performing an assay with or without the device, and methods for manufacturing the device are provided. The device is a continuous liquid flow channel having a proximal and a distal end, with a detection membrane in fluid communication with the distal end of the flow channel. Interspersed between the assay buffer and detection membrane, and continuous with the liquid flow channel, are a sample delivery means, one or more reservoirs containing the reagents necessary for conducting the assay, and, optionally, mixing or incubation reservoirs for combining the sample and reagents. The geometry of the liquid flow channel regulates the flow rate of the liquids through the channel, thereby controlling incubation, mixing and reaction time. The preferred detection membrane is an immunochromatographic test strip containing immobilized reagents. The detection of labeled reagent in a particular area of the detection membrane reflects the presence or relative amount of analyte in the sample. Detection may be achieved visually. One or more liquid flow channels may be contained within a single housing for simultaneous, consecutive, or comparative sample analysis.
摘要:
This invention relates to field of screening and diagnosing adverse local tissue reaction (ALTR) in a subject who has received a joint replacement by measuring the level of a nucleic acid or protein biomarker that are elevated in patients suffering from ALTR, even those with no symptoms. The early diagnosis of the ALTR can lead to its treatment and thus, the prevention of implant failure caused by the ALTR. The elevated proteins and genes are also the basis for treatment for ALTR and provide targets for drug development and basic research.
摘要:
Compositions and methods utilizing Raman Spectroscopy to detect and determine, directly or indirectly, the concentration of water treatment polymers in water or methods for measuring or quantifying the amount of water treatment polymer in a sample, such as an industrial, commercial, or municipal water sample, are provided. The compositions contain water treatment polymers that have been modified to include one or more Raman-active functional groups. The methods utilize Raman spectroscopy to detect the presence or amount of water treatment polymer either directly or indirectly. The method provides the ability to detect or measure the amount of active water treatment polymer remaining in the sample.