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公开(公告)号：US07585963B2
公开(公告)日：2009-09-08
申请号：US10733042
申请日：2003-12-11
申请人： Markley C. Leavitt , Jeff Rapp , Robert Ivarie , Arthur Karnuah
发明人： Markley C. Leavitt , Jeff Rapp , Robert Ivarie , Arthur Karnuah
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2217/05 , A01K2217/072 , A01K2227/30 , A01K2267/01 , C07K16/00 , C07K2317/11 , C07K2317/21 , C12N2799/02 , C12N2800/10 , C12N2800/204 , C12N2800/206 , C12N2830/90
摘要： The present invention provides isolated and recombinant avian nucleic acid molecules comprising at least one avian MAR and an avian nucleic acid sequence encoding an ovalbumin transcriptional regulatory region. The isolated nucleic acid of the present invention is useful for reducing chromosomal positional effects upon the transcription of a transgene operably linked to the ovalbumin transcriptional regulatory region and transfected into a recipient avian cell The recombinant nucleic acid molecules of the present invention may further comprise a polyadenylation signal sequence or an avian 3′ domain, and optionally, an internal ribosome entry site for expression of an operably linked heterologous nucleic acid insert in a transfected avian cell.
摘要翻译： 本发明提供分离和重组的禽类核酸分子，其包含至少一种禽类MAR和编码卵白蛋白转录调节区的禽类核酸序列。 本发明的分离的核酸可用于降低可操作地连接到卵白蛋白转录调节区并转染到接受者禽类细胞中的转基因的转录上的染色体位置效应。本发明的重组核酸分子还可以包含聚腺苷酸化 信号序列或鸟类3'结构域，以及任选的内部核糖体进入位点，用于在转染的禽类细胞中表达可操作地连接的异源核酸插入物。
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公开(公告)号：US20090210951A1
公开(公告)日：2009-08-20
申请号：US12386223
申请日：2009-04-15
申请人： Robert Ivarie , Arthur Karnuah , Markley C. Leavitt , Jeff Rapp
发明人： Robert Ivarie , Arthur Karnuah , Markley C. Leavitt , Jeff Rapp
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2217/05 , A01K2217/072 , A01K2227/30 , A01K2267/01 , C07K16/00 , C07K2317/11 , C07K2317/21 , C12N2799/02 , C12N2800/10 , C12N2800/204 , C12N2800/206 , C12N2830/90
摘要： The invention includes delivering to an avian a nucleic acid molecule which includes nucleotide sequence of an ovalbumin gene expression controlling region and a heterologous coding sequence operably linked to the gene expression controlling region wherein the heterologous coding sequence is expressed in a cell of the avian.
摘要翻译： 本发明包括递送给禽类核酸分子，其包括卵白蛋白基因表达控制区的核苷酸序列和与基因表达控制区可操作地连接的异源编码序列，其中异源编码序列在禽的细胞中表达。
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公开(公告)号：US20060130170A1
公开(公告)日：2006-06-15
申请号：US10733042
申请日：2003-12-11
申请人： Markley Leavitt , Jeff Rapp , Robert Ivarie , Arthur Karnuah
发明人： Markley Leavitt , Jeff Rapp , Robert Ivarie , Arthur Karnuah
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2217/05 , A01K2217/072 , A01K2227/30 , A01K2267/01 , C07K16/00 , C07K2317/11 , C07K2317/21 , C12N2799/02 , C12N2800/10 , C12N2800/204 , C12N2800/206 , C12N2830/90
摘要： The present invention provides isolated and recombinant avian nucleic acid molecules comprising at least one avian MAR and an avian nucleic acid sequence encoding an ovalbumin transcriptional regulatory region. The isolated nucleic acid of the present invention is useful for reducing chromosomal positional effects upon the transcription of a transgene operably linked to the ovalbumin transcriptional regulatory region and transfected into a recipient avian cell The recombinant nucleic cid molecules of the present invention may further comprise a polyadenylation signal sequence or an avian 3′ domain, and optionally, an internal ribosome entry site for expression of an operably linked heterologous nucleic acid insert in a transfected avian cell.
摘要翻译： 本发明提供分离和重组的禽类核酸分子，其包含至少一种禽类MAR和编码卵白蛋白转录调节区的禽类核酸序列。 本发明的分离的核酸可用于降低可操作地连接到卵白蛋白转录调节区并转染入受体禽类细胞的转基因的转录中的染色体位置效应。本发明的重组核酸分子还可以包含多聚腺苷酸化 信号序列或鸟类3'结构域，以及任选的内部核糖体进入位点，用于在转染的禽类细胞中表达可操作地连接的异源核酸插入物。
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公开(公告)号：US09452342B2
公开(公告)日：2016-09-27
申请号：US15132397
申请日：2016-04-19
申请人： Jeff Rapp
发明人： Jeff Rapp
CPC分类号： A63C5/075 , A63C2203/065 , B62B13/043 , B62B13/06 , B62B17/02 , B62B17/061 , B62B17/062 , B62B17/063
摘要： A skiing apparatus including an upper frame member, a lower seat member, a handle member, a lower frame, a ski attachment member, an upper shock absorbing connector member, and a lower shock absorbing connector member. The lower seat member has a medial portion connected to the upper frame member. The handle member is connected to the upper frame member in fixed relation to the lower seat member. The lower frame member is connected to the lower seat member. The ski attachment member is connected to the lower frame member and adapted to be detachably connected to a ski. The upper shock absorbing connector member is connected to the lower seat member. The lower shock absorbing connector member is connected to the lower frame member.
摘要翻译： 一种滑雪装置，包括上框架构件，下座椅构件，手柄构件，下框架，滑雪板附接构件，上部减震连接器构件和下部减震连接器构件。 下座椅构件具有连接到上框架构件的中间部分。 手柄构件与下座椅构件固定地连接到上框架构件。 下框架构件连接到下座椅构件。 滑雪板安装构件连接到下框架构件并且适于可拆卸地连接到滑雪板。 上部减震连接器构件连接到下座椅构件。 下部减震连接器构件连接到下框架构件。
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公开(公告)号：US20160229442A1
公开(公告)日：2016-08-11
申请号：US15132397
申请日：2016-04-19
申请人： Jeff Rapp
发明人： Jeff Rapp
CPC分类号： A63C5/075 , A63C2203/065 , B62B13/043 , B62B13/06 , B62B17/02 , B62B17/061 , B62B17/062 , B62B17/063
摘要： A skiing apparatus including an upper frame member, a lower seat member, a handle member, a lower frame, a ski attachment member, an upper shock absorbing connector member, and a lower shock absorbing connector member. The lower seat member has a medial portion connected to the upper frame member. The handle member is connected to the upper frame member in fixed relation to the lower seat member. The lower frame member is connected to the lower seat member. The ski attachment member is connected to the lower frame member and adapted to be detachably connected to a ski. The upper shock absorbing connector member is connected to the lower seat member. The lower shock absorbing connector member is connected to the lower frame member.
摘要翻译： 一种滑雪装置，包括上框架构件，下座椅构件，手柄构件，下框架，滑雪板附接构件，上部减震连接器构件和下部减震连接器构件。 下座椅构件具有连接到上框架构件的中间部分。 手柄构件与下座椅构件固定地连接到上框架构件。 下框架构件连接到下座椅构件。 滑雪板安装构件连接到下框架构件并且适于可拆卸地连接到滑雪板。 上部减震连接器构件连接到下座椅构件。 下部减震连接器构件连接到下框架构件。
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公开(公告)号：US09333982B1
公开(公告)日：2016-05-10
申请号：US14523416
申请日：2014-10-24
申请人： Jeff Rapp
发明人： Jeff Rapp
CPC分类号： B62B13/005 , B62B13/043 , B62B17/062 , B62B17/063
摘要： A snow skiing apparatus which may include an upper frame member, a lower seat member connected to the upper frame member, a handle member connected to the upper frame member, a lower frame member connected to the upper frame member, and a ski attachment member connected to the lower frame member and adapted to be detachably connected to a ski.
摘要翻译： 一种滑雪装置，其可以包括上框架构件，连接到上框架构件的下座构件，连接到上框架构件的手柄构件，连接到上框架构件的下框架构件和连接到上框架构件的滑雪板附件 并且适于可拆卸地连接到滑雪板。
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公开(公告)号：US09637156B2
公开(公告)日：2017-05-02
申请号：US15277679
申请日：2016-09-27
申请人： Jeff Rapp
发明人： Jeff Rapp
CPC分类号： B62B17/062 , A63C5/075 , A63C2203/065 , B62B13/005 , B62B13/043 , B62B13/06 , B62B17/02 , B62B17/061 , B62B17/063
摘要： A skiing apparatus including a lower seat member, a lower frame member, a ski attachment member, and upper shock absorbing connector member, a lower shock absorbing connector member, and a lower storage member. The lower frame member may connect to the lower seat member. The ski attachment member may connect to the lower frame member and be adapted to be detachably connected to a ski. The upper shock absorbing connector member may connect to the lower seat member. The lower shock absorbing connector member may connect to the lower frame member. The lower storage member may connect to the lower seat member.
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公开(公告)号：US20170015344A1
公开(公告)日：2017-01-19
申请号：US15277679
申请日：2016-09-27
申请人： Jeff Rapp
发明人： Jeff Rapp
CPC分类号： B62B17/062 , A63C5/075 , A63C2203/065 , B62B13/005 , B62B13/043 , B62B13/06 , B62B17/02 , B62B17/061 , B62B17/063
摘要： A skiing apparatus including a lower seat member, a lower frame member, a ski attachment member, and upper shock absorbing connector member, a lower shock absorbing connector member, and a lower storage member. The lower frame member may connect to the lower seat member. The ski attachment member may connect to the lower frame member and be adapted to be detachably connected to a ski. The upper shock absorbing connector member may connect to the lower seat member. The lower shock absorbing connector member may connect to the lower frame member. The lower storage member may connect to the lower seat member.
摘要翻译： 一种滑雪装置，包括下部座椅构件，下部框架构件，滑雪板安装构件和上部减震连接器构件，下部减震连接器构件和下部存储构件。 下框架构件可以连接到下座椅构件。 滑雪板安装构件可以连接到下框架构件并且适于可拆卸地连接到滑雪板。 上部减震连接器构件可以连接到下座椅构件。 下部减震连接器构件可以连接到下框架构件。 下存储构件可以连接到下座椅构件。
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公开(公告)号：US20160114825A1
公开(公告)日：2016-04-28
申请号：US14523416
申请日：2014-10-24
申请人： Jeff Rapp
发明人： Jeff Rapp
CPC分类号： B62B13/005 , B62B13/043 , B62B17/062 , B62B17/063
摘要： A snow skiing apparatus which may include an upper frame member, a lower seat member connected to the upper frame member, a handle member connected to the upper frame member, a lower frame member connected to the upper frame member, and a ski attachment member connected to the lower frame member and adapted to be detachably connected to a ski.
摘要翻译： 一种滑雪装置，其可以包括上框架构件，连接到上框架构件的下座构件，连接到上框架构件的手柄构件，连接到上框架构件的下框架构件和连接到上框架构件的滑雪板附件 并且适于可拆卸地连接到滑雪板。
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