Chicken ovalbumin nucleotide sequence
    9.
    发明授权
    Chicken ovalbumin nucleotide sequence 有权
    鸡卵白蛋白核苷酸序列

    公开(公告)号:US07585963B2

    公开(公告)日:2009-09-08

    申请号:US10733042

    申请日:2003-12-11

    IPC分类号: C01H21/04 C12N15/00 C12N5/00

    摘要: The present invention provides isolated and recombinant avian nucleic acid molecules comprising at least one avian MAR and an avian nucleic acid sequence encoding an ovalbumin transcriptional regulatory region. The isolated nucleic acid of the present invention is useful for reducing chromosomal positional effects upon the transcription of a transgene operably linked to the ovalbumin transcriptional regulatory region and transfected into a recipient avian cell The recombinant nucleic acid molecules of the present invention may further comprise a polyadenylation signal sequence or an avian 3′ domain, and optionally, an internal ribosome entry site for expression of an operably linked heterologous nucleic acid insert in a transfected avian cell.

    摘要翻译: 本发明提供分离和重组的禽类核酸分子,其包含至少一种禽类MAR和编码卵白蛋白转录调节区的禽类核酸序列。 本发明的分离的核酸可用于降低可操作地连接到卵白蛋白转录调节区并转染到接受者禽类细胞中的转基因的转录上的染色体位置效应。本发明的重组核酸分子还可以包含聚腺苷酸化 信号序列或鸟类3'结构域,以及任选的内部核糖体进入位点,用于在转染的禽类细胞中表达可操作地连接的异源核酸插入物。