公开(公告)号：US09994865B2

公开(公告)日：2018-06-12

申请号：US14895125

申请日：2014-06-04

申请人： The Johns Hopkins University ,  Virginia Commonwealth University

发明人： Paul B. Fisher ,  Swadesh K. Das ,  Mitchell E. Menezes ,  Devanand Sarkar ,  Martin G. Pomper

IPC分类号： A61K48/00 ,  C12N15/85 ,  C12N15/64 ,  C07K14/47 ,  G01N33/50 ,  G01N33/574 ,  A61K38/16 ,  A61K38/17 ,  A61K38/20 ,  A61K41/00 ,  A61K49/00 ,  A61N7/00 ,  C07K14/36 ,  C07K14/465 ,  C07K14/54 ,  C12N7/00 ,  C12N15/86 ,  C12N15/62 ,  B82Y5/00 ,  B82Y15/00

CPC分类号： C12N15/85 ,  A61K38/164 ,  A61K38/1703 ,  A61K38/20 ,  A61K41/0028 ,  A61K48/00 ,  A61K48/0058 ,  A61K48/0075 ,  A61K49/00 ,  A61N7/00 ,  A61N2007/0039 ,  B82Y5/00 ,  B82Y15/00 ,  C07K14/36 ,  C07K14/465 ,  C07K14/47 ,  C07K14/54 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/035 ,  C12N7/00 ,  C12N15/625 ,  C12N15/64 ,  C12N15/86 ,  C12N2710/10043 ,  C12N2710/10343 ,  C12N2799/02 ,  C12N2799/022 ,  C12N2800/22 ,  C12N2810/855 ,  C12N2830/002 ,  C12N2830/008 ,  G01N33/50 ,  G01N33/574

摘要： The presently disclosed subject matter relates to compositions and methods directed to cancer theranostic nucleic acid constructs that permit simultaneous cancer-specific viral replication, expression of a diagnostic gene product, and expression of a therapeutic gene.

公开(公告)号：US08846578B2

公开(公告)日：2014-09-30

申请号：US12905824

申请日：2010-10-15

申请人： Paul McCray ,  Morgan Maeder ,  Jae Keith Joung

发明人： Paul McCray ,  Morgan Maeder ,  Jae Keith Joung

IPC分类号： C40B50/06 ,  C12N9/22 ,  C07K14/47 ,  C12N15/10 ,  A61K38/00

CPC分类号： C12N9/22 ,  A61K38/00 ,  C07K7/06 ,  C07K14/4702 ,  C07K14/4703 ,  C12N15/1082 ,  C12N15/86 ,  C12N15/907 ,  C12N2799/02 ,  C12N2799/027 ,  C12N2800/80

摘要： The present invention provides new zinc finger proteins and zinc finger nuclease (ZFNs) that find particular using in repairing the cystic fibrosis transmembrane conductance regulator (CFTR) gene.

摘要翻译： 本发明提供了特别用于修复囊性纤维化跨膜传导调节因子（CFTR）基因的新锌指蛋白和锌指核酸酶（ZFN）。

公开(公告)号：US20080293141A1

公开(公告)日：2008-11-27

申请号：US11663990

申请日：2005-09-28

申请人： Hideto Chono ,  Hiromi Okuyama ,  Tomoe Egashira ,  Nobuto Koyama ,  Junichi Mineno ,  Ikunoshin Kato

发明人： Hideto Chono ,  Hiromi Okuyama ,  Tomoe Egashira ,  Nobuto Koyama ,  Junichi Mineno ,  Ikunoshin Kato

IPC分类号： C12N15/87 ,  C12N5/06 ,  C12N15/63

CPC分类号： C12N9/1051 ,  C12N15/86 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2799/02 ,  C12N2810/10

摘要： The N-acetylglucosaminyltransferase III activity is enhanced in a cell carrying retrovirus-origin gag-pol gene and env gene. By constructing a retrovirus vector with the use of the above cell, a retrovirus vector having a modified sugar chain structure can be obtained. The retrovirus vector constructed by this method shows a high infection efficiency particularly in the presence of a functional substance.

摘要翻译： 在携带逆转录病毒起始gag-pol基因和env基因的细胞中N-乙酰葡糖胺基转移酶III活性增强。 通过使用上述细胞构建逆转录病毒载体，可以获得具有修饰的糖链结构的逆转录病毒载体。 通过该方法构建的逆转录病毒载体特别是在功能性物质存在下显示高感染效率。

公开(公告)号：US20240182534A1

公开(公告)日：2024-06-06

申请号：US18484279

申请日：2023-10-10

申请人： Universitat Autònoma de Barcelona

发明人： Fàtima BOSCH TUBERT ,  Verónica JIMÉNEZ CENZANO ,  Claudia JAMBRINA PALLARES

IPC分类号： C07K14/50 ,  A01K67/0275 ,  A61K35/761 ,  C12N15/113 ,  C12N15/85 ,  C12N15/86

CPC分类号： C07K14/50 ,  A01K67/0275 ,  A61K35/761 ,  C12N15/113 ,  C12N15/86 ,  A01K2207/25 ,  A01K2227/105 ,  A01K2267/0362 ,  C12N2015/8518 ,  C12N2310/141 ,  C12N2750/14143 ,  C12N2799/02

摘要： The invention relates to a viral expression construct and related viral vector and nucleic acid molecule and composition and to their use wherein said construct and vector are suitable for expression in a mammal and comprise a nucleotide sequence encoding a Fibroblast growth factor 21 (FGF21) to be expressed in liver, adipose tissue and/or skeletal muscle.

公开(公告)号：US20190194249A1

公开(公告)日：2019-06-27

申请号：US16329812

申请日：2017-08-31

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： Hideki WATANABE ,  Shinya HONDA

IPC分类号： C07K1/22 ,  C07K7/06 ,  C12N15/62 ,  C07K16/06 ,  C12N7/00

CPC分类号： C07K1/22 ,  C07K7/06 ,  C07K16/00 ,  C07K16/065 ,  C07K2317/32 ,  C07K2317/34 ,  C07K2317/522 ,  C07K2319/70 ,  C12N7/00 ,  C12N15/09 ,  C12N15/62 ,  C12N2799/02

摘要： The present invention relates to a novel polypeptide having affinity for proteins partially including a CH1-CL domain forming a non-native three-dimensional structure and capable of being suitably used for detecting, immobilizing, or removing these proteins and relates to use of the polypeptide. Specifically, disclosed are a polypeptide consisting of an amino acid sequence represented by any one of the following formulas 1 to 3: (1) P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 1), (2) Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 4), and (3) P—N-S-G-G-G-G-S-Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 7) (wherein x represents an amino acid residue; and brackets represent any one of the amino acid residues within the brackets), and a method of using the polypeptide to detect, purify, or remove a protein partially including a CH1-CL domain forming a non-native three-dimensional structure.

公开(公告)号：US10072274B2

公开(公告)日：2018-09-11

申请号：US15399588

申请日：2017-01-05

申请人： BAVARIAN NORDIC A/S

发明人： Robin Steigerwald

IPC分类号： C12N15/86 ,  C12N15/863

CPC分类号： C12N15/8636 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/11022 ,  C12N2760/14122 ,  C12N2799/02 ,  C12N2800/22

摘要： The invention relates to vectors comprising two or more homologous nucleotide sequences and methods for generating them. The invention concerns substituting bases in the homologous nucleotide sequences with different bases that do not alter the encoded amino acid sequence. The invention allows for the reduction of intramolecular recombination between homologous nucleotide sequences, in particular in mammalian cells. The invention further relates to nucleotide sequences containing substituted bases.

公开(公告)号：US20160108429A1

公开(公告)日：2016-04-21

申请号：US14895125

申请日：2014-06-04

申请人： VIRGINIA COMMONWEALTH UNIVERSITY ,  THE JOHNS HOPKINS UNIVERSITY

发明人： Paul B. FISHER ,  Swadesh K. Das ,  Mitchell E. MENEZES ,  Devanand Sarkar ,  Martin G. POMPER

IPC分类号： C12N15/85 ,  A61K38/20 ,  C07K14/465 ,  C07K14/36 ,  C07K14/54 ,  A61N7/00 ,  A61K48/00 ,  A61K38/17 ,  A61K38/16 ,  C12N15/86 ,  C12N7/00 ,  A61K49/00 ,  A61K41/00

CPC分类号： C12N15/85 ,  A61K38/164 ,  A61K38/1703 ,  A61K38/20 ,  A61K41/0028 ,  A61K48/00 ,  A61K48/0058 ,  A61K48/0075 ,  A61K49/00 ,  A61N7/00 ,  A61N2007/0039 ,  B82Y5/00 ,  B82Y15/00 ,  C07K14/36 ,  C07K14/465 ,  C07K14/47 ,  C07K14/54 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/035 ,  C12N7/00 ,  C12N15/625 ,  C12N15/64 ,  C12N15/86 ,  C12N2710/10043 ,  C12N2710/10343 ,  C12N2799/02 ,  C12N2799/022 ,  C12N2800/22 ,  C12N2810/855 ,  C12N2830/002 ,  C12N2830/008 ,  G01N33/50 ,  G01N33/574

摘要： The presently disclosed subject matter relates to compositions and methods directed to cancer theranostic nucleic acid constructs that permit simultaneous cancer-specific viral replication, expression of a diagnostic gene product, and expression of a therapeutic gene.

摘要翻译： 目前公开的主题涉及涉及癌细胞核酸构建体的组合物和方法，其允许同时进行癌症特异性病毒复制，诊断基因产物的表达和治疗基因的表达。

公开(公告)号：US20100172888A1

公开(公告)日：2010-07-08

申请号：US12092522

申请日：2006-11-09

申请人： Michael I. Nishimura ,  Hugo R. Rosen

发明人： Michael I. Nishimura ,  Hugo R. Rosen

IPC分类号： A61K35/12 ,  C12N5/10 ,  C07H21/04 ,  C12N15/63 ,  A61P35/04 ,  C12P21/06 ,  C12N5/00

CPC分类号： C07K14/7051 ,  A61K48/005 ,  C07K14/005 ,  C12N2740/10043 ,  C12N2770/24222 ,  C12N2799/02 ,  C12N2799/06

摘要： Provided are cells expressing HCV epitope-reactive recombinant T cell receptors useful in the treatment and/or prevention of acute or chronic HCV and HCV-related conditions or malignancies. The invention further provides methods of preparing HCV epitope-reactive T cell receptors and methods of treatment using cells expressing HCV epitope-reactive recombinant T cell receptors. Polynucleotides, constructs and vectors encoding HCV epitope-reactive recombinant T cell receptors are also provided.

摘要翻译： 提供表达用于治疗和/或预防急性或慢性HCV和HCV相关病症或恶性肿瘤的HCV表位反应性重组T细胞受体的细胞。 本发明还提供了制备HCV表位反应性T细胞受体的方法和使用表达HCV表位反应性重组T细胞受体的细胞进行治疗的方法。 还提供了编码HCV表位反应性重组T细胞受体的多核苷酸，构建体和载体。

公开(公告)号：US20170211098A1

公开(公告)日：2017-07-27

申请号：US15399588

申请日：2017-01-05

申请人： BAVARIAN NORDIC A/S

发明人： Robin STEIGERWALD

IPC分类号： C12N15/863

CPC分类号： C12N15/8636 ,  C12N15/86 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2710/24151 ,  C12N2740/11022 ,  C12N2760/14122 ,  C12N2799/02 ,  C12N2800/22

摘要： The invention relates to vectors comprising two or more homologous nucleotide sequences and methods for generating them. The invention concerns substituting bases in the homologous nucleotide sequences with different bases that do not alter the encoded amino acid sequence. The invention allows for the reduction of intramolecular recombination between homologous nucleotide sequences, in particular in mammalian cells. The invention further relates to nucleotide sequences containing substituted bases.

公开(公告)号：US09061076B2

公开(公告)日：2015-06-23

申请号：US12601736

申请日：2008-05-22

申请人： Stefan Franzen ,  Richard Guenther ,  Steven A. Lommel ,  LiNa Loo

发明人： Stefan Franzen ,  Richard Guenther ,  Steven A. Lommel ,  LiNa Loo

IPC分类号： A61K31/704 ,  A61P35/00 ,  A61K51/00 ,  C12N15/09 ,  A61K47/48 ,  A61K9/51 ,  B82Y5/00 ,  C12N7/00

CPC分类号： A61K47/48776 ,  A61K9/5184 ,  A61K47/6425 ,  A61K47/645 ,  A61K47/6901 ,  B82Y5/00 ,  C12N7/00 ,  C12N15/09 ,  C12N2770/38023 ,  C12N2770/38043 ,  C12N2799/02 ,  C12N2810/855

摘要： This invention is a process for the manufacture of a plant viral capsid to be used for the targeted delivery of therapeutics to diseased cells. The process uses a plant virus as the starting material. The choice of the plant virus overcomes a problem in the manufacture of a uniform starting material. The final product has an advantage over other plant virus-based delivery systems in that the plant virus selected has a natural structure that is resistant to breakdown during the delivery process. This system takes advantage of the reversible divalent cation switch that this capsid employs to assemble and disassemble.

摘要翻译： 本发明是一种用于制造用于向病变细胞靶向递送治疗剂的植物病毒衣壳的方法。 该过程使用植物病毒作为起始材料。 植物病毒的选择克服了制造均匀原料的问题。 最终产品优于其他基于植物病毒的递送系统，因为所选择的植物病毒具有在递送过程中耐破坏的天然结构。 该系统利用该衣壳用于组装和拆卸的可逆二价阳离子开关。