公开(公告)号：US20050053944A1

公开(公告)日：2005-03-10

申请号：US10636343

申请日：2003-08-07

申请人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

发明人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6813 ,  G01N27/447 ,  G01N33/566 ,  C07K14/47

CPC分类号： G01N27/44726 ,  C12Q1/6813 ,  G01N27/447 ,  C12Q2525/113 ,  C12Q2523/113 ,  C12Q2565/125 ,  C12Q2565/629 ,  C12Q2565/107

摘要： A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.

摘要翻译： 一种方法组合物和装置，用于通过使单链核酸样品与具有与靶序列互补的序列的可检测PNA探针接触来杂交和分离样品中具有所需靶序列的分子，使得靶序列（如果存在） 将与可检测的探针杂交以形成可检测的双链体，然后通过电泳将变性培养基中的双链体与未结合的样品组分分离。 本发明还涉及用于通过使样品与可检测的PNA探针接触来在单链核酸及其互补链的混合样品中杂交和分离具有所需靶序列的分子的方法组合物和装置。