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1.发明申请Methods and kit for hybridization anaylsis using peptide nucleic acid probes 审中-公开使用肽核酸探针进行杂交分析的方法和试剂盒公开(公告)号：US20050053944A1
公开(公告)日：2005-03-10
申请号：US10636343
申请日：2003-08-07
申请人： Martin Fuchs , Michael Egholm , Heather O'Keefe , Xian-Wei Yao
发明人： Martin Fuchs , Michael Egholm , Heather O'Keefe , Xian-Wei Yao
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , C12Q1/6813 , G01N27/447 , G01N33/566 , C07K14/47
CPC分类号： G01N27/44726 , C12Q1/6813 , G01N27/447 , C12Q2525/113 , C12Q2523/113 , C12Q2565/125 , C12Q2565/629 , C12Q2565/107
摘要： A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.
摘要翻译： 一种方法组合物和装置，用于通过使单链核酸样品与具有与靶序列互补的序列的可检测PNA探针接触来杂交和分离样品中具有所需靶序列的分子，使得靶序列（如果存在） 将与可检测的探针杂交以形成可检测的双链体，然后通过电泳将变性培养基中的双链体与未结合的样品组分分离。 本发明还涉及用于通过使样品与可检测的PNA探针接触来在单链核酸及其互补链的混合样品中杂交和分离具有所需靶序列的分子的方法组合物和装置。
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2.发明授权Binary probe and clamp composition and methods for target hybridization detection 有权二进制探针和夹钳组成及靶杂交检测方法公开(公告)号：US08067165B2
公开(公告)日：2011-11-29
申请号：US12538823
申请日：2009-08-10
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q2549/126 , C12Q2537/119 , C12Q2525/179
摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.
摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。
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3.发明申请System and method for improved processing of nucleic acids for production of sequencable libraries 审中-公开用于生产可序列文库的核酸改进处理系统和方法公开(公告)号：US20110003701A1
公开(公告)日：2011-01-06
申请号：US12380139
申请日：2009-02-23
申请人： Gianni Calogero Ferreri , Jan F. Simons , Michael Todd Ronan , Michael Egholm , Brian C. Godwin , David Roderick Riches , Stephen Kyle Hutchison , Michael S. Braverman , Melinda D. Palmer , Jeffrey Jeddeloh , Jacob Kitzman , Thomas J. Albert
发明人： Gianni Calogero Ferreri , Jan F. Simons , Michael Todd Ronan , Michael Egholm , Brian C. Godwin , David Roderick Riches , Stephen Kyle Hutchison , Michael S. Braverman , Melinda D. Palmer , Jeffrey Jeddeloh , Jacob Kitzman , Thomas J. Albert
CPC分类号： C12N15/66
摘要： An embodiment of an adaptor element for efficient target processing is described that comprises a semi-complementary double stranded nucleic acid adaptor comprising a non-complementary region and a complementary region, where the non-complementary region comprises a first amplification primer site and a second amplification primer site and the complementary region comprises a sequencing primer site and one or more inosine species.
摘要翻译： 描述了用于有效靶处理的衔接子元件的实施方案，其包含半互补双链核酸衔接子，其包含非互补区域和互补区域，其中所述非互补区域包含第一扩增引物位点和第二扩增 引物位点和互补区包含测序引物位点和一种或多种肌苷物质。
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公开(公告)号：US07601499B2
公开(公告)日：2009-10-13
申请号：US11448462
申请日：2006-06-06
申请人： Jan Berka , Zhoutao Chen , Michael Egholm , Brian C. Godwin , Stephen Kyle Hutchison , John Harris Leamon , Gary James Sarkis , Jan Fredrik Simons
发明人： Jan Berka , Zhoutao Chen , Michael Egholm , Brian C. Godwin , Stephen Kyle Hutchison , John Harris Leamon , Gary James Sarkis , Jan Fredrik Simons
CPC分类号： C12Q1/6806 , C12N15/10 , C12N15/1093 , C12Q1/6809 , C12Q1/6869 , C12Q2537/155 , C12Q2525/191 , C12Q2521/301 , C12Q2533/107 , C12Q2531/125 , C12Q2521/319 , C12Q2521/125 , C12Q2565/518 , C12Q2563/131
摘要： The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.
摘要翻译： 本发明提供了制备靶核酸片段以产生包含靶核酸两端的较小核酸的方法。 具体地，本发明提供克隆和DNA操作策略，以将大靶核酸的两端分离成单个小型DNA构建体用于快速克隆，测序或扩增。
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公开(公告)号：US07223833B1
公开(公告)日：2007-05-29
申请号：US08319411
申请日：1994-10-06
申请人： Peter Nielsen , Ole Buchardt , Soren Holst Sonnechsen , Jesper Lohse , Michael Egholm , Muthiah Manoharan , John Kiely , Michael Griffith , Kelly Sprankle
发明人： Peter Nielsen , Ole Buchardt , Soren Holst Sonnechsen , Jesper Lohse , Michael Egholm , Muthiah Manoharan , John Kiely , Michael Griffith , Kelly Sprankle
CPC分类号： C07K14/003 , C12Q1/6832 , C12Q2525/107
摘要： A novel class of peptide nucleic acids are described which include a conjugate attached thereto. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译： 描述了一类新的肽核酸，其包括与其连接的缀合物。 肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。
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公开(公告)号：US06710163B1
公开(公告)日：2004-03-23
申请号：US08468719
申请日：1995-06-06
IPC分类号： C12Q168
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA更强地结合互补的ssDNA和RNA链。 肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。
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公开(公告)号：US06613873B1
公开(公告)日：2003-09-02
申请号：US09337304
申请日：1999-06-21
IPC分类号： A61K3800
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , C12Q2535/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary DNA and RNA strands more strongly than a corresponding DNA strand, and exhibit increased sequence specificity and binding affinity. The peptide nucleic acids of the invention comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone. Some PNAs of the invention also contain C1-C8 alkylamine side chains.
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公开(公告)号：US06414112B1
公开(公告)日：2002-07-02
申请号：US08686114
申请日：1996-07-24
IPC分类号： A61K3800
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , C12Q2535/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary DNA and RNA strands more strongly than a corresponding DNA strand, and exhibit increased sequence specificity and binding affinity. The peptide nucleic acids of the invention comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone. Some PNAs of the invention also contain C1-C8 alkylamine side chains.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA链更强地结合互补DNA和RNA链，并表现出增加的序列特异性和结合亲和力。 本发明的肽核酸包含选自天然存在的核碱基和连接到聚酰胺主链上的非天然存在的核碱基的配体。 本发明的一些PNA还含有C1-C8烷基胺侧链。
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公开(公告)号：US06395474B1
公开(公告)日：2002-05-28
申请号：US08108591
申请日：1993-11-22
IPC分类号： C12Q168
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA更强地结合互补的ssDNA和RNA链。 肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。
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公开(公告)号：US06228982B1
公开(公告)日：2001-05-08
申请号：US08088661
申请日：1993-07-02
申请人： Benget Norden , Pernilla Wittung , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf Berg
发明人： Benget Norden , Pernilla Wittung , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf Berg
IPC分类号： C07K500
CPC分类号： C12N15/113 , A61K38/00 , B82Y5/00 , C07H21/00 , C07K14/003 , C12N15/1132 , C12N15/1135 , C12N2310/13 , C12N2310/15 , C12N2310/3181 , C12N2310/3513 , C12Q1/6813 , C12Q2525/107 , C12Q2537/119
摘要： A novel class of compounds, known as peptide nucleic acids, form double-stranded structures with one another and with ssDNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译： 一类新的化合物，称为肽核酸，形成双链结构，并与ssDNA形成双链结构。 肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。
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