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    Targeted transfection of cells using a biotinylated dendrimer
    1.
    发明授权
    Targeted transfection of cells using a biotinylated dendrimer 有权
    使用生物素化的树枝状大分子靶向转染细胞

    公开(公告)号：US07192744B2

    公开(公告)日：2007-03-20

    申请号：US10240981

    申请日：2001-04-03

    申请人： Martin Weber ,  Jörg Dennig ,  Christoph Erbacher

    发明人： Martin Weber ,  Jörg Dennig ,  Christoph Erbacher

    IPC分类号： C12P19/34 ,  C12N15/00 ,  C12N15/10 ,  C12N15/11

    CPC分类号： B82Y5/00 ,  A61K47/645 ,  A61K47/6898 ,  C08G83/004 ,  C12N15/87

    摘要： The present invention relates to processes for the targeted transfection of cells, compositions which may be used for such processes, and corresponding pharmaceutical compositions for gene therapy. In particular, the invention relates to a process for introducing nucleic acid into cells comprising the steps of (a) mixing a nucleic acid with a dendrimer, in which a proportion of the dendrimer molecules are biotinylated; (b) mixing the resulting complex of nucleic acid and dendrimer with a second complex consisting of avidin or streptavidin and a biotinylated target-specific binding molecule; and (c) incubating the complex formed in step (b) with cells. Dendrimers which are well suited to the present invention include, for example, partially solvolysed polyamidoamine (PAMAM) dendrimers. Target-specific binding molecules are, in particular, cell-type-specific markers of the cell surface of the target cells.

    摘要翻译： 本发明涉及细胞靶向转染的方法，可用于此类方法的组合物和用于基因治疗的相应药物组合物。 特别地，本发明涉及将核酸引入细胞的方法，包括以下步骤：（a）将核酸与树状聚合物混合，其中一部分树枝状聚合物分子被生物素化; （b）将所得到的核酸和树状聚合物复合物与由抗生物素蛋白或链霉抗生物素蛋白和生物素化靶特异性结合分子组成的第二配合物混合; 和（c）将步骤（b）中形成的络合物与细胞一起培养。 适合本发明的树枝状聚合物包括例如部分溶解溶解的聚酰胺胺（PAMAM）树枝状聚合物。 靶特异性结合分子尤其是靶细胞的细胞表面的细胞型特异性标记。