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11 条记录 (耗时 0.016 秒)

    Thermostable polymerases from Thermococcus pacificus
    2.
    发明授权
    Thermostable polymerases from Thermococcus pacificus 失效
    热太阳能热稳定聚合酶

    公开(公告)号：US08124391B2

    公开(公告)日：2012-02-28

    申请号：US10398011

    申请日：2001-10-05

    申请人： Katja Decker ,  Dirk Löffert ,  Jie Kang

    发明人： Katja Decker ,  Dirk Löffert ,  Jie Kang

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252

    摘要： The invention relates to a thermostable polymerase based on thermococcus pacificus; DNA molecules which code for one such polymerase; expression vectors; host cells; methods for producing one such polymerase and the use thereof for polymerising nucleic acid, especially in the polymerase chain reaction.

    摘要翻译： 本发明涉及一种基于热电球菌的热稳定聚合酶; 编码一种这样的聚合酶的DNA分子; 表达载体 宿主细胞; 用于生产一种这样的聚合酶的方法及其用于聚合核酸的用途，特别是在聚合酶链式反应中。

    TRANSFECTION MICROARRAYS
    4.
    发明申请
    TRANSFECTION MICROARRAYS 审中-公开
    转移微量

    公开(公告)号：US20100075422A1

    公开(公告)日：2010-03-25

    申请号：US12517356

    申请日：2007-12-13

    申请人： Wolfgang Bielke ,  Jie Kang ,  Karsten Reihs

    发明人： Wolfgang Bielke ,  Jie Kang ,  Karsten Reihs

    IPC分类号： C12N15/10 ,  C40B40/06

    CPC分类号： C12N15/87 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/161 ,  B01L2300/166 ,  B82Y5/00 ,  C12M35/00

    摘要： A transfection method of introducing sample molecules into cells is provided, comprising: (a) using a substrate comprising at least one sample spot area which is surrounded by an hydrophobic area; (b) wherein sample molecules are applied to said at least one sample spot area, thereby placing said sample molecules in the discrete location of said sample spot area; (c) applying cells to be transfected onto the substrate and incubation under appropriate conditions for entry of the sample molecules into said cells; (d) whereby at least a portion of said sample molecules are introduced into said cells.

    摘要翻译： 提供了将样品分子引入细胞的转染方法，包括：（a）使用包含至少一个由疏水区域包围的样品斑点区域的底物; （b）其中将样品分子施加到所述至少一个样品斑点区域，从而将所述样品分子放置在所述样品斑点区域的离散位置; （c）将待转染的细胞施用到底物上并在适当的条件下温育以使样品分子进入所述细胞; （d），其中至少一部分所述样品分子被引入所述细胞。

    Generation and amplification of nucleic acids from ribonucleic acids
    6.
    发明授权
    Generation and amplification of nucleic acids from ribonucleic acids 有权
    来自核糖核酸的核酸的产生和扩增

    公开(公告)号：US06300069B1

    公开(公告)日：2001-10-09

    申请号：US09304452

    申请日：1999-05-03

    申请人： Andreas Missel ,  Dirk Löffert ,  Jie Kang ,  Christian Korfhage

    发明人： Andreas Missel ,  Dirk Löffert ,  Jie Kang ,  Christian Korfhage

    IPC分类号： C12Q168

    CPC分类号： C12N15/1096 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2521/107

    摘要： Novel compositions and methods useful for the generation of nucleic acids from a ribonucleic acid template and further nucleic acid replication are disclosed. It is shown that the generation and amplification of nucleic acids by methods that utilize two or more different polymerases, such as reverse transcriptase-polymerase chain reaction (RT-PCR), are dramatically more sensitive and efficient in the presence of a homopolymeric nucleic acid. Homopolymeric nucleic acids have been found to reduce or negate the inhibitory effect reverse transcriptases have on DNA polymerase activity. It is demonstrated that this inhibition-relieving effect of homopolymeric nucleic acids is general in nature; independent of the chemical species of homopolymer used, or the chemical composition of the polymerization reaction mixture.

    摘要翻译： 公开了用于从核糖核酸模板产生核酸和进一步核酸复制的新型组合物和方法。 显示通过利用两种或多种不同聚合酶的方法（如逆转录酶 - 聚合酶链反应（RT-PCR））在均聚核酸存在下显着更灵敏和有效地产生和扩增核酸。 已经发现均聚核酸减少或否定逆转录酶对DNA聚合酶活性的抑制作用。 证明均聚核酸的这种抑制作用是一般性的; 独立于所用均聚物的化学物质，或聚合反应混合物的化学组成。

    Thermostable chimeric nucleic acid polymerases and uses thereof
    7.
    发明授权
    Thermostable chimeric nucleic acid polymerases and uses thereof 失效
    热稳定嵌合核酸聚合酶及其用途

    公开(公告)号：US08637288B2

    公开(公告)日：2014-01-28

    申请号：US10216682

    申请日：2002-08-08

    申请人： Dirk Löffert ,  Andreas Missel ,  Jie Kang

    发明人： Dirk Löffert ,  Andreas Missel ,  Jie Kang

    IPC分类号： C12N9/12

    CPC分类号： C12P19/34 ,  C07K2319/00 ,  C12N9/1252

    摘要： Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable.

    摘要翻译： 公开了新型热稳定嵌合核酸聚合酶及其产生和使用的方法。 显示这些嵌合核酸聚合酶，例如DNA聚合酶，可以使用从不同蛋白质分离的化学合成的酶活性结构域构建。 证明本发明的嵌合核酸聚合酶具有其组分结构域的化学和物理性质（例如外切核酸酶活性，热稳定性），并且嵌合聚合酶是热稳定的。

    Rapid and simple process for isolation of circular nucleic acids
    8.
    发明授权
    Rapid and simple process for isolation of circular nucleic acids 有权
    快速简单的环状核酸分离方法

    公开(公告)号：US06821757B2

    公开(公告)日：2004-11-23

    申请号：US09341119

    申请日：1999-11-18

    申请人： Philippe Sauer ,  Jie Kang

    发明人： Philippe Sauer ,  Jie Kang

    IPC分类号： C12P1934

    CPC分类号： C12N15/1006 ,  C07B2200/11 ,  C07H21/00 ,  C40B40/00

    摘要： A method for separating and/or isolating circular nucleic acids from a mixture having different species of nucleic acids other than circular nucleic acids wherein the mixture is treated under alkaline conditions at a pH>8 with a solid matrix consisting essentially of a silica material in presence of at least one chaotropic substance.

    摘要翻译： 用于从具有不同环状核酸种类的混合物分离和/或分离循环核酸的方法，其中所述混合物在pH> 8的碱性条件下处理，其中固体基质主要由二氧化硅材料组成， 的至少一种离液剂。