公开(公告)号：US4650556A

公开(公告)日：1987-03-17

申请号：US710131

申请日：1985-03-11

申请人： Masakazu Hashiue ,  Masashi Ogawa ,  Daijiro Nishio

发明人： Masakazu Hashiue ,  Masashi Ogawa ,  Daijiro Nishio

IPC分类号： G01N27/447 ,  G01N27/26 ,  G01N27/28

CPC分类号： G01N27/44747

摘要： A medium for electrophoresis which is substantially free from smiling effect or is reduced in the smiling effect, in which both side portions along the direction of electrophoresis are made thicker than the center portion. A support preferably employed for the preparation of the medium is also disclosed.

摘要翻译： 基本上没有微笑效果的电泳介质或微笑效果的降低，其中沿着电泳方向的两侧部分比中心部分厚。 还公开了优选用于制备介质的载体。

公开(公告)号：US4640759A

公开(公告)日：1987-02-03

申请号：US710130

申请日：1985-03-11

申请人： Masakazu Hashiue ,  Masashi Ogawa

发明人： Masakazu Hashiue ,  Masashi Ogawa

IPC分类号： G01N27/447 ,  G01N27/28

CPC分类号： G01N27/44747

摘要： A process for electrophoresis employing a medium wherein the potential gradient along the direction of electrophoresis is made larger at both side portions of the medium than at the center portion thereof. A medium for employable for said process in that the length of the both side portions along the direction of electrophoresis is made shorter than that of the center portion is disclosed. Also disclosed is a support for the medium.

摘要翻译： 使用介质的电泳方法，其中沿着电泳方向的电位梯度在介质的两侧部比在其中心部分更大。 用于所述方法的介质是沿着电泳方向的两侧部分的长度比中心部分的长度被公开。 还公开了对该介质的支持。

公开(公告)号：US4737259A

公开(公告)日：1988-04-12

申请号：US948401

申请日：1986-12-29

申请人： Masashi Ogawa ,  Masakazu Hashiue ,  Yuzo Mizobuchi

发明人： Masashi Ogawa ,  Masakazu Hashiue ,  Yuzo Mizobuchi

IPC分类号： G01N27/447 ,  C07K3/14

CPC分类号： G01N27/44747 ,  Y10T428/31765

摘要： An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for the determination of the base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, a nonconductive metal oxide layer, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.

摘要翻译： 适用于生物聚合物如蛋白质电泳的电泳元件，以及用于测定DNA，RNA，其片段及其衍生物的碱基序列，其包含塑料载体，非导电金属氧化物层和 电泳介质层，其包含通过在水存在下交联聚合丙烯酰胺化合物和交联剂而形成的聚丙烯酰胺凝胶水溶液，其以该顺序重叠。 电泳介质层可含有水溶性聚合物和琼脂糖。 中间层可以含有改性剂，例如阴离子表面活性剂，甲酰胺或脲。

公开(公告)号：US4922101A

公开(公告)日：1990-05-01

申请号：US308592

申请日：1989-02-10

申请人： Masakazu Hashiue

发明人： Masakazu Hashiue

IPC分类号： G01B11/00 ,  C07H21/04 ,  C12N1/00 ,  C12N15/09 ,  G01T1/29 ,  G03B27/62 ,  G03G15/04 ,  H01J65/04 ,  H04N1/04

CPC分类号： G01T1/2014

摘要： A new method of photoelectrically reading out a radiation image of one or more spots which is stored and recorded on a stimulable phosphor sheet, to determine position of the spot on the phosphor sheet, comprises the steps of: scanning the stimulable phosphor sheet under rotation twice with stimulating rays giving a linear irradiation area on a surface of the phosphor sheet to form in total an irradiated area in the form of a ring; detecting stimulated emissions caused by a series of the irradiations of stimulating rays separately for each scanning, for obtaining information as to whether the spot is present or absent on the irradiated linear area; and locating the position of the spot on the phosphor sheet by combining the information of the spot obtained in the twice scanning.

摘要翻译： 一种光电读出存储并记录在可刺激的荧光体片上的一个或多个斑点的放射线图像以确定荧光体片上的斑点的位置的新方法包括以下步骤：在可旋转的荧光体片材上扫描两次 刺激光线在荧光体片的表面上产生线性照射区域，以形成环状的照射区域; 检测针对每次扫描分别对刺激光线进行一系列照射而产生的受激发射，以获得关于该照射线性区域上是否存在斑点的信息; 并通过组合在两次扫描中获得的斑点的信息来将斑点的位置定位在荧光体片上。

公开(公告)号：US4941092A

公开(公告)日：1990-07-10

申请号：US866355

申请日：1986-05-23

申请人： Makoto Hara ,  Masakazu Hashiue ,  Kazuyoshi Tanaka

发明人： Makoto Hara ,  Masakazu Hashiue ,  Kazuyoshi Tanaka

IPC分类号： C12Q1/68 ,  G01T1/29

CPC分类号： G01T1/2942 ,  C12Q1/6869

摘要： A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium,which comprises steps of:(1) detecting at least two bands continuously in the lower part of each resolved row and numbering the bands consecutively in order from the lower end;(2) obtaining correlation of a distance between the detected bands in the resolving direction with the band's number and predicting positions of undetected bands in the resolving direction from the correlation;(3) detecting at least one band on the resolved rows on the basis of the predicted positions and numbering the bands consecutively;(4) obtaining the correlation of the distance between the bands with the band's number for the already detected bands including the band newly detected in the step (3), and predicting positions of undetected bands from the correlation; and(5) repeating in order the steps (3) and (4) to thereby detect all bands on the resolved rows.

摘要翻译： 一种用于通过使数字信号进行信号处理来确定核酸碱基序列的信号处理方法，所述数字信号对应于通过分解碱基特异性DNA片段或碱基特异性RNA片段的混合物而形成的多个分辨行的放射自显影图 在支撑介质上具有一维方向的放射性元素，其包括以下步骤：（1）在每个分辨行的下部连续检测至少两个频带，并从下端依次对频带进行编号; （2）获得分辨方向上检测的频带之间的距离与频带数的相关性，并从相关性预测解析方向上的未检测频带的位置; （3）基于预测位置检测解决的行上的至少一个频带，并连续编号频带; （4）获得包括在步骤（3）中新检测的带的已经检测的频带的频带之间的距离与带的距离的相关性，以及从相关性预测未检测到的频带的位置; 和（5）按顺序重复步骤（3）和（4），从而检测解决的行上的所有频带。

公开(公告)号：US4301374A

公开(公告)日：1981-11-17

申请号：US95138

申请日：1979-11-16

申请人： Masakazu Hashiue

发明人： Masakazu Hashiue

IPC分类号： G11B7/085 ,  G11B7/135 ,  G06K7/10 ,  G11B7/00

CPC分类号： G11B7/085 ,  G11B7/1381

摘要： An optical information storage system having a data record with control markings therein and an optical readout system including a scanner. The readout system uses a laser light source and focusing optics to direct the light onto the data record for modulation and subsequent readout of the modulated light. Shutter elements are interposed to intercept the transmission of light in response to control signals in the data. The shutter, of the electronic type such as lead lanthanum zirconate titanate, may be positioned either between the source of light and the scanner or between the data record and the readout.

摘要翻译： 一种具有其中具有控制标记的数据记录的光学信息存储系统和包括扫描仪的光学读出系统。 读出系统使用激光光源和聚焦光学器件将光引导到数据记录上，用于调制和随后的调制光的读出。 插入快门元件以响应于数据中的控制信号来截取光的传输。 诸如锆钛酸铅的电子类型的快门可以位于光源和扫描仪之间或数据记录和读出器之间。

公开(公告)号：US4569903A

公开(公告)日：1986-02-11

申请号：US664938

申请日：1984-10-25

申请人： Masakazu Hashiue ,  Koji Ochiai

发明人： Masakazu Hashiue ,  Koji Ochiai

IPC分类号： G03C5/04 ,  G03C5/26 ,  G03C5/32 ,  G03C5/40 ,  G11B7/0045 ,  G11B7/251 ,  G11B7/28 ,  G03C5/16

CPC分类号： G03C5/04 ,  G03C5/26 ,  G03C5/32 ,  G03C5/40 ,  G11B7/00455 ,  G11B7/251 ,  G11B7/28 ,  Y10S430/146

摘要： A recording medium for reflective readout in an optical information recording system. The medium comprises a substrate such as PET or PMMA on emulsion layer of silver halide material and an optical protective layer. The emulsion is exposed to a laser source to form a latent data image and is then developed to exhibit a glossy silver metallic image. Playback uses established systems.

摘要翻译： 一种用于光学信息记录系统中的反射读出的记录介质。 该介质包括在卤化银材料的乳剂层和光学保护层上的诸如PET或PMMA的基底。 将乳剂暴露于激光源以形成潜在数据图像，然后显影以显示光泽的银金属图像。 播放使用已建立的系统。

公开(公告)号：US4882488A

公开(公告)日：1989-11-21

申请号：US251481

申请日：1988-09-30

申请人： Masakazu Hashiue

发明人： Masakazu Hashiue

IPC分类号： G01T1/29 ,  H04N1/029 ,  H04N1/036 ,  H04N1/10 ,  H04N1/207

CPC分类号： G03B42/08 ,  G01T1/2014 ,  H04N1/029 ,  H04N1/036 ,  H04N1/10 ,  H04N1/207 ,  H04N2201/0424

摘要： A stimulable phosphor sheet carrying a radiation image stored thereon is mounted on a first rotary plate, and a stimulating light beam is projected onto the sheet while the first rotary plate is rotated, thereby effecting main scanning of the sheet by the stimulating light beam. The stimulating light beam is moved relative to the first rotary plate in a radial direction of the first rotary plate to effect sub-scanning of the sheet by the stimulating light beam. Light emitted by the sheet upon stimulation by the stimulating light beam is photoelectrically detected by a photoelectric converter, thereby obtaining an image signal. Recording light beam is modulated on the basis of the image signal, and the modulated recording light beam is projected onto a recording medium mounted on a second rotary plate while the second rotary plate is rotated in synchronization with the main scanning of the recording medium by the stimulating light beam to effect main scanning of the recording medium by the recording light beam. The recording light beam is moved relative to the second rotary plate in a radial direction of the second rotary plate in synchronization with the sub-scanning of the sheet by the stimulating light beam to effect sub-scanning of the recording medium by the recording light beam.

摘要翻译： 携带其上存储的放射线图像的可刺激的磷光体片安装在第一旋转板上，并且在旋转第一旋转板的同时将刺激光束投影到片材上，从而通过刺激光束进行片材的主扫描。 刺激光束相对于第一旋转板沿着第一旋转板的径向方向移动，以通过刺激光束对片材进行副扫描。 由刺激光束刺激时由片材发射的光由光电转换器光电检测，从而获得图像信号。 基于图像信号调制记录光束，并且将调制记录光束投射到安装在第二旋转板上的记录介质上，同时第二旋转板与记录介质的主扫描同步旋转 刺激光束通过记录光束实现记录介质的主扫描。 记录光束通过刺激光束与片材的副扫描同步地沿着第二旋转板的径向相对于第二旋转板移动，以通过记录光束进行记录介质的副扫描 。

公开(公告)号：US4837687A

公开(公告)日：1989-06-06

申请号：US844967

申请日：1986-03-27

申请人： Kazuyoshi Tanaka ,  Masakazu Hashiue

发明人： Kazuyoshi Tanaka ,  Masakazu Hashiue

IPC分类号： G01T1/29

CPC分类号： G01T1/2942

摘要： A method for analyzing autoradiography by subjecting digital signals corresponding to an autoradiograph having information on two-dimensional location of radioactively labeled substances resolved on a support medium to signal processing, to obtain the locational information on the radioactively labeled substances in the form of visual indicia, which includes the steps of:(1) electrically displaying the autoradiograph in the form of an image on the basis of the digital signals;(2) extracting signals in a given area along resolved rows of the radioactively labeled substances on the basis of input information on the rows determined from the display image; and(3) subjecting the extracted signals to one-dimensional signal processing to detect resolved portions.

摘要翻译： 一种用于分析放射自显影的方法，该数字信号对应于具有在载体介质上分解的放射性标记物质的二维位置的信息的放射自显影仪进行信号处理，以获得视觉标记形式的放射性标记物质的位置信息， 其包括以下步骤：（1）基于数字信号以图像的形式电子地显示放射自显影图像; （2）基于从显示图像确定的行的输入信息，沿着解决的放射性标记物质的行来提取给定区域中的信号; 和（3）对所提取的信号进行一维信号处理以检测分辨部分。

公开(公告)号：US4734581A

公开(公告)日：1988-03-29

申请号：US904865

申请日：1986-09-08

申请人： Masakazu Hashiue

发明人： Masakazu Hashiue

IPC分类号： G03B42/02 ,  G03C5/16 ,  G01T1/10

CPC分类号： G03B42/02 ,  G03C5/16

摘要： An autoradiographic process for obtaining information on one or two dimensional location of radioactively labeled substances originating from an organism and resolved on a support medium, which comprises:(1) causing a stimulable phosphor sheet comprising a stimulable phosphor, which is combined with said support medium in layers or has been integrated with said support medium, to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in the support medium; and(2) placing a photosensitive material on said stimulable phosphor sheet, and irradiating the phosphor sheet with stimulating rays to release the radiation energy stored in the phosphor sheet as stimulated emission, whereby the photosensitive material is exposed to the stimulated emission to obtain locational information on he radioactively labeled substances as an image on the photosensitive material. Other autoradiographic processes which further include photoelectrically detecting the stimulated emission to obtain the locational information as electric signals are also disclosed.

摘要翻译： 一种放射自显影方法，用于获得源自生物体并在载体介质上分辨的放射性标记物质的一维或二维位置的信息，其包括：（1）引起包含可刺激磷光体的可刺激磷光体片，其与所述支持介质组合 或者已经与所述支撑介质集成，以吸收由载体介质中的放射性标记物质发射的辐射能量的至少一部分; 和（2）将感光材料放置在所述可刺激的荧光体片材上，并用刺激光线照射荧光体片材以释放作为受激发射的荧光体片材中存储的辐射能量，由此感光材料暴露于受激发射以获得位置信息 他将放射性标记的物质作为感光材料上的图像。 还公开了进一步包括光电检测受激发射以获得位置信息作为电信号的其它放射自显影方法。