Method of enzymatically measuring hydrogen peroxide and reagent therefor
    1.
    发明授权
    Method of enzymatically measuring hydrogen peroxide and reagent therefor 失效
    酶法测定过氧化氢及其试剂的方法

    公开(公告)号:US5474908A

    公开(公告)日:1995-12-12

    申请号:US797011

    申请日:1991-11-25

    CPC分类号: C12Q1/28 C12Q1/30 C12Q2326/32

    摘要: A method of enzymatically measuring hydrogen peroxide by developing a color with use of 3-methyl-2-benzothiazolinoehydrazone as a coupler and in combination with an oxidative color developing reagent with peroxidase, and a reagent therefor is described. Catalase is added to the 3-methyl-2-benzothiazolinonehydrazone reagent as a stabilizer and the reaction is carried out in the presence of ethylenediaminetetraacetic acid or an analogue thereof. The color developing reaction may be carried out in neutral or weak alkali conditions. A reagent is described which comprises a buffer, 3-methyl-2-benzothiazolinohydrazone, and catalase.

    摘要翻译: 描述了使用3-甲基-2-苯并噻唑腙腙作为偶联剂并与具有过氧化物酶的氧化显色剂组合的显色测定过氧化氢的方法及其试剂。 将过氧化氢酶作为稳定剂加入到3-甲基-2-苯并噻唑啉酮腙试剂中,反应在乙二胺四乙酸或其类似物的存在下进行。 显色反应可以在中性或弱碱性条件下进行。 描述了包含缓冲剂,3-甲基-2-苯并噻唑腙和过氧化氢酶的试剂。