公开(公告)号：US20180272346A1

公开(公告)日：2018-09-27

申请号：US15926900

申请日：2018-03-20

Applicant: Massachusetts Institute of Technology

Inventor： Linda G. Griffith ,  David Trumper ,  Collin Edington ,  Gaurav Rohatgi ,  Duncan Freake ,  Luis Soenksen ,  Timothy Kassis ,  Mohan Brij Bhushan

IPC: B01L3/00 ,  G01F23/26

CPC classification number: B01L3/502715 ,  B01J2219/00182 ,  B01J2219/00306 ,  B01J2219/00355 ,  B01J2219/00479 ,  B01J2219/00889 ,  B01J2219/00952 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/086 ,  C12M23/04 ,  C12M23/16 ,  C12M23/42 ,  F04B19/006 ,  F04B23/06 ,  F04B43/043 ,  F04B43/12 ,  G01F23/263

Abstract: Fluidic multiwell bioreactors are provided as a microphysiological platform for in vitro investigation of multi-organ crosstalks with microbiome for an extended period of time of at least weeks and months. The platform has one or more improvements over existing bioreactors, including on-board pumping for pneumatically driven fluid flow, a redesigned spillway for self-leveling from source to sink, a non-contact built-in fluid level sensing device, precise control on fluid flow profile and partitioning, and facile reconfigurations such as daisy chaining and multilayer stacking. The platform supports the culture of multiple organs together with microbiome in a microphysiological, interacted systems, suitable for a wide range of biomedical applications including systemic toxicity studies and physiology-based pharmacokinetic and pharmacodynamic predictions. A process to fabricate the bioreactors is also provided.