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1.
公开(公告)号:US20080220418A1
公开(公告)日:2008-09-11
申请号:US11664367
申请日:2005-09-30
申请人: Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
发明人: Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
CPC分类号: C12Q1/6806 , C12N15/1003 , C12Q1/6858 , C12Q1/686 , C12Q2523/125 , C12Q2527/137 , C12Q2527/149 , C12Q2531/107
摘要: Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.
摘要翻译: 本发明的方面涉及从归档样品(例如石蜡包埋和/或固定组织活组织检查等)提供DNA片段的组合物和方法。 具体方面提供了分离DNA的高产率以及大部分DNA由长DNA片段组成并且分离的基因组DNA不含相关或交联的污染物如蛋白质,肽,氨基酸或RNA的方法 。 这些方法是容易的,具有成本效益的,其特征在于重现性和可靠性高。 特定方面提供用于提供衍生自归档样品的DNA片段的方法,其中在例如扩增步骤之前的DNA的产量为至少20%,并且可扩增至约1000个碱基对的扩增子。
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2.
公开(公告)号:US08679745B2
公开(公告)日:2014-03-25
申请号:US11664367
申请日:2005-09-30
申请人: Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Kluth Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
发明人: Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Kluth Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
CPC分类号: C12Q1/6806 , C12N15/1003 , C12Q1/6858 , C12Q1/686 , C12Q2523/125 , C12Q2527/137 , C12Q2527/149 , C12Q2531/107
摘要: Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.
摘要翻译: 本发明的方面涉及从归档样品(例如石蜡包埋和/或固定组织活组织检查等)提供DNA片段的组合物和方法。 具体方面提供了分离DNA的高产率以及大部分DNA由长DNA片段组成并且分离的基因组DNA不含相关或交联的污染物如蛋白质,肽,氨基酸或RNA的方法 。 该方法简便,成本效益高,重现性和可靠性高。 特定方面提供用于提供衍生自归档样品的DNA片段的方法,其中在例如扩增步骤之前的DNA的产量为至少20%,并且可扩增至约1000个碱基对的扩增子。
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公开(公告)号:US07447671B2
公开(公告)日:2008-11-04
申请号:US10692503
申请日:2003-10-24
IPC分类号: G06N5/00
摘要: Configuration of a measurement task on a protocol tester includes displaying a problem field on a display device, the problem field having several network elements of a telecommunication network; graphically activating one of the network elements by a user; displaying different measurement tasks on the display device which are possible with respect to the activated network element; graphically selecting one of the measurement tasks by the user; and entering further parameters for the configuration of the measurement task, proceeding from one level of the network element level by level in lower levels, with the parameters not specified by the user being occupied by standard values.
摘要翻译: 在协议测试器上配置测量任务包括在显示设备上显示问题字段,问题字段具有电信网络的几个网络元件; 用户图形地激活网络元素之一; 在显示设备上显示相对于激活的网元可能的不同测量任务; 用户以图形方式选择测量任务之一; 并为测量任务的配置输入进一步的参数,从较低级别的级别的一级级别开始,用户未指定的参数被标准值占用。
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公开(公告)号:US20070033455A1
公开(公告)日:2007-02-08
申请号:US11349895
申请日:2006-02-07
申请人: Ute Wagner , Mike Wiedemann , Christian Schoenfeld , Christian Zander , Michael Bluemche , Heinz-Joachim Laake , Christian Hain , Kristian Martens , Karsten Kiehlmann , Elisabeth Muncher
发明人: Ute Wagner , Mike Wiedemann , Christian Schoenfeld , Christian Zander , Michael Bluemche , Heinz-Joachim Laake , Christian Hain , Kristian Martens , Karsten Kiehlmann , Elisabeth Muncher
IPC分类号: G01R31/28
摘要: A load testing apparatus and method has a display unit for the presentation of data that relate to a load test of a telecommunication network. The display includes a graphical user interface with the load test being divided into several test phases and on the graphical user interface functionalities being assigned to these test phases. The load testing apparatus further has a storage device into which user identifiers are enterable, and the functionalities are pooled into groups so that for each identifier there one or more groups of functionalities may be enabled.
摘要翻译: 负载测试装置和方法具有用于呈现与电信网络的负载测试相关的数据的显示单元。 显示器包括图形用户界面,负载测试分为几个测试阶段,以及分配给这些测试阶段的图形用户界面功能。 负载测试装置还具有可将用户标识符输入的存储装置,并且将功能汇集成组,使得对于每个标识符,可以启用一组或多组功能。
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公开(公告)号:US07496814B2
公开(公告)日:2009-02-24
申请号:US11349895
申请日:2006-02-07
申请人: Ute Wagner , Mike Wiedemann , Christian Schoenfeld , Christian Zander , Michael Bluemche , Heinz-Joachim Laake , Christian Hain , Kristian Martens , Karsten Kiehlmann , Elisabeth A. Muncher
发明人: Ute Wagner , Mike Wiedemann , Christian Schoenfeld , Christian Zander , Michael Bluemche , Heinz-Joachim Laake , Christian Hain , Kristian Martens , Karsten Kiehlmann , Elisabeth A. Muncher
IPC分类号: G01R31/28
摘要: A load testing apparatus and method has a display unit for the presentation of data that relate to a load test of a telecommunication network. The display includes a graphical user interface with the load test being divided into several test phases and on the graphical user interface functionalities being assigned to these test phases. The load testing apparatus further has a storage device into which user identifiers are enterable, and the functionalities are pooled into groups so that for each identifier there one or more groups of functionalities may be enabled.
摘要翻译: 负载测试装置和方法具有用于呈现与电信网络的负载测试相关的数据的显示单元。 显示器包括图形用户界面,负载测试分为几个测试阶段,以及分配给这些测试阶段的图形用户界面功能。 负载测试装置还具有可将用户标识符输入的存储装置,并且将功能汇集成组,使得对于每个标识符,可以启用一个或多个功能组。
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