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    Method For Determining The Methylation Rate of a Nucleic Acid
    5.
    发明申请
    Method For Determining The Methylation Rate of a Nucleic Acid 审中-公开
    确定核酸甲基化速率的方法

    公开(公告)号：US20100003680A1

    公开(公告)日：2010-01-07

    申请号：US12309555

    申请日：2006-07-18

    申请人： Joern Lewin ,  Ralf Lesche ,  Matthias Schuster ,  Dimo Dietrich

    发明人： Joern Lewin ,  Ralf Lesche ,  Matthias Schuster ,  Dimo Dietrich

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6869

    摘要： The invention relates to a method for quantitatively determining the methylation rate of a nucleic acid through sequencing. According to the invention, the method comprises at least the following steps: a) treating the nucleic acid with a chemical reagent or an enzyme containing solution, whereby the base pairing behavior of methylated cytosine bases and/or unmethylated cytosine bases of the nucleic acid are altered such that methylated cytosine bases become distinguishable from unmethylated cytosine bases, and b) introducing into the nucleic acid at least one base for generating a sequencing signal to be used as a reference signal for normalization, and c) sequencing the nucleic acid, whereby a signal from each cytosine base of the nucleic acid, or a signal from each guanine base of the nucleic acid and a reference signal from the at least on introduced base is obtained, and d) normalizing the signal obtained from each cytosine base of the nucleic acid, or the signal obtained from each guanine base of the nucleic acid to the reference signal from the at least one introduced base.

    摘要翻译： 本发明涉及通过测序定量测定核酸的甲基化速率的方法。 根据本发明，该方法至少包括以下步骤：a）用化学试剂或含酶溶液处理核酸，由此核酸的甲基化胞嘧啶碱基和/或未甲基化的胞嘧啶碱基的碱基配对行为是 改变为使得甲基化胞嘧啶碱基与非甲基化胞嘧啶碱基区分开，以及b）将至少一个碱基引入核酸，以产生用作标准化参考信号的测序信号，和c）对核酸进行测序，由此 获得来自核酸的每个胞嘧啶碱基的信号或来自核酸的每个鸟嘌呤碱基的信号和来自至少一个引入的碱基的参考信号，以及d）使从核酸的每个胞嘧啶碱基获得的信号归一化 或从核酸的每个鸟嘌呤碱基获得的信号至至少一个引入的碱基的参考信号。

    Method for analysis of cytosine methylation
    6.
    发明申请
    Method for analysis of cytosine methylation 审中-公开
    胞嘧啶甲基化分析方法

    公开(公告)号：US20090075251A1

    公开(公告)日：2009-03-19

    申请号：US10594013

    申请日：2005-03-24

    申请人： Dimo Dietrich ,  Philipp Schatz ,  Matthias Schuster ,  Antje Kluth

    发明人： Dimo Dietrich ,  Philipp Schatz ,  Matthias Schuster ,  Antje Kluth

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6858 ,  C12Q2525/143 ,  C12Q2523/125

    摘要： A method for the analysis of cytosine methylations in DNA is described. Here, the DNA to be investigated is first chemically or enzymatically converted. Then a promoter is introduced into the DNA. After this, the DNA is converted to RNA. The methylation pattern of the DNA can be concluded in different ways by means of an analysis of the RNA. The RNA is preferably fragmented chemically or enzymatically prior to the analysis, whereby the fragmenting results depend on the methylation pattern of the DNA. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer disorders and other diseases associated with a modification of the methylation pattern.

    摘要翻译： 描述了分析DNA中胞嘧啶甲基化的方法。 这里，待研究的DNA首先被化学或酶转化。 然后将启动子引入DNA。 之后，将DNA转化为RNA。 DNA的甲基化模式可以通过RNA的分析以不同的方式得出结论。 优选在分析之前将RNA片段化学或酶促，由此片段化结果取决于DNA的甲基化模式。 根据本发明的方法特别适合于与甲基化模式的改变相关的癌症病症和其它疾病的诊断和预后。

    Method for investigating cytosine methylations in dna
    7.
    发明申请
    Method for investigating cytosine methylations in dna 审中-公开
    在dna中调查胞嘧啶甲基化的方法

    公开(公告)号：US20090208941A1

    公开(公告)日：2009-08-20

    申请号：US11989081

    申请日：2006-07-19

    申请人： Kurt Berlin ,  Matthias Schuster ,  Philipp Schatz

    发明人： Kurt Berlin ,  Matthias Schuster ,  Philipp Schatz

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827

    摘要： A method for sensitive and specific detection of cytosine methylation is described wherein the DNA to be analyzed is subjected to initial enrichment. More specifically, said enrichment can be effected in a methylation-specific, sequence-specific or origin-specific fashion. Thereafter, the enriched DNA is converted in a methylation-specific fashion. The converted DNA can be analyzed using various methods, particularly real-time PCR methods.

    摘要翻译： 描述了用于敏感和特异性检测胞嘧啶甲基化的方法，其中待分析的DNA经受初始富集。 更具体地，所述富集可以以甲基化特异性，序列特异性或起始特异性方式实现。 此后，富集的DNA以甲基化特异性方式转化。 可以使用各种方法，特别是实时PCR方法分析转化的DNA。

    Method for the detection of nucleic acid sequences by means of crackable probe molecules
    8.
    发明申请
    Method for the detection of nucleic acid sequences by means of crackable probe molecules 审中-公开
    通过可裂解的探针分子检测核酸序列的方法

    公开(公告)号：US20060166201A1

    公开(公告)日：2006-07-27

    申请号：US10526277

    申请日：2003-09-01

    申请人： Philipp Schatz ,  Matthias Schuster ,  Kurt Berlin

    发明人： Philipp Schatz ,  Matthias Schuster ,  Kurt Berlin

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q2563/167

    摘要： The present invention describes a method for the detection of nucleic acid sequences, which is characterized in that the following steps are conducted: a) at least one nucleic acid is bound to a solid phase; b) probe molecules are hybridized to the nucleic acids in a sequence-specific manner, whereby the probe molecules are provided with a cleavable bond and a mass label, which is specific for the probe molecule; c) removal of the unhybridized probe molecules; d) contacting of the hybridized probe molecules with a matrix, which cleaves said cleavable bonds and at the same time serves as the matrix in a MALDI mass spectrometer; e) detection of the mass label at those positions where the nucleic acid was bound.

    摘要翻译： 本发明描述了用于检测核酸序列的方法，其特征在于进行以下步骤：a）至少一种核酸与固相结合; b）探针分子以序列特异性方式与核酸杂交，由此提供探针分子具有可切割键和对于探针分子是特异性的质量标记; c）去除未杂交的探针分子; d）将杂交的探针分子与基质接触，所述基质切割所述可切割键，并同时用作MALDI质谱仪中的基质; e）在核酸结合的那些位置检测质量标签。