Affinity biotinylation
    2.
    发明授权
    Affinity biotinylation 失效
    亲和生物素化

    公开(公告)号:US6150123A

    公开(公告)日:2000-11-21

    申请号:US146587

    申请日:1998-09-03

    申请人: Antonio Cosma

    发明人: Antonio Cosma

    摘要: The present invention relates to a method for biotinylating a desired subset of a larger population of proteins, wherein the subset of proteins is first selectively bound to a solid phase, thereby separating it from undesired proteins of the population, and then, still bound to solid phase, it is biotinylated. Unreacted biotin may then be washed from the solid phase, and the biotinylated protein may be uncoupled from the solid phase.

    摘要翻译: 本发明涉及一种用于生物素化更大群体蛋白质的所需子集的方法,其中蛋白质子集首先选择性地结合固相,从而将其与群体的不需要的蛋白质分离,然后仍然结合固体 相,它是生物素化的。 然后可以将未反应的生物素从固相洗涤,并且生物素化的蛋白质可以从固相中脱离。