公开(公告)号：US20230257802A1

公开(公告)日：2023-08-17

申请号：US17990445

申请日：2022-11-18

Applicant: McMaster University

Inventor： Monsur Ali ,  Dawn White ,  Saeed Mohammadi ,  John D. Brennan ,  Yingfu Li ,  Alfredo Capretta

IPC: C12Q1/6837 ,  C12Q1/689

CPC classification number: C12Q1/6837 ,  C12Q1/689

Abstract: This disclosure relates to catalytic nucleic acid probes, biosensors and lateral flow biosensor systems and methods and kits of using the probes, biosensors and lateral flow biosensor systems for detecting microorganisms such as Staphylococcus aureus.

公开(公告)号：US20230295635A1

公开(公告)日：2023-09-21

申请号：US17966575

申请日：2022-10-14

Applicant: McMaster University

Inventor： John D. Brennan ,  Monsur Ali ,  Michael Wolfe ,  Dawn White ,  Manali Mukherjee ,  Parameswaran Nair ,  Alfredo Capretta

IPC: C12N15/115 ,  C12Q1/28

CPC classification number: C12N15/115 ,  C12Q1/28 ,  C12N2310/16

Abstract: This disclosure relates to DNA aptamers that bind eosinophil peroxidase, including uses thereof, such as in eosinophil peroxidase detection assays. Also provided is a method for detecting the presence of eosinophil peroxidase in a sample, using the DNA aptamers that bind eosinophil peroxidase. Further provided is a kit for detecting the presence of eosinophil peroxidase in a sample, using the DNA aptamers that bind eosinophil peroxidase.

公开(公告)号：US20250101437A1

公开(公告)日：2025-03-27

申请号：US18728342

申请日：2023-01-11

Applicant: McMaster University

Inventor： Yingfu Li ,  John Brennan ,  Zijie Zhang ,  Jiuxing Li ,  Jimmy Gu ,  Dawn White ,  Bruno Salena ,  Matthew Miller ,  Deborah Yamamura

IPC: C12N15/115 ,  C12N15/10 ,  C40B30/04 ,  G01N33/569

Abstract: This disclosure relates to a method of identifying or producing an aptamer capable of binding to at least two target analyte variants, the method comprises generating a mixture of systematic evolution of ligands by exponential enrichment (SELEX)-selected aptamers by at least one-round of SELEX with a SELEX-compatible aptamer pool against the at least two target analyte variants in parallel or sequentially, sequencing the mixture of SELEX-selected aptamers that form complexes with each of the at least two target analyte variants by high-throughput sequencing, aligning the mixture of SELEX-selected aptamers that form complexes with each of the at least two target analyte variants by sequence alignment analysis, and identifying or producing the aptamer capable of binding to the at least two target analyte variants. Aptamers thereof and uses of the aptamers thereof are also disclosed.