公开(公告)号：US20240272148A1

公开(公告)日：2024-08-15

申请号：US18437875

申请日：2024-02-09

申请人： McMaster University

发明人： Carlos Filipe ,  John D. Brennan ,  Robert Pelton ,  Sana Jahanshahi-Anbuhi ,  Yingfu Li

IPC分类号： G01N33/543 ,  C12Q1/66 ,  C12Q1/686 ,  G01N21/77 ,  G01N21/78 ,  G01N33/548

CPC分类号： G01N33/5436 ,  C12Q1/66 ,  C12Q1/686 ,  G01N21/78 ,  G01N33/548 ,  G01N2021/7786 ,  G01N2333/922

摘要： The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

公开(公告)号：US20230295635A1

公开(公告)日：2023-09-21

申请号：US17966575

申请日：2022-10-14

申请人： McMaster University

发明人： John D. Brennan ,  Monsur Ali ,  Michael Wolfe ,  Dawn White ,  Manali Mukherjee ,  Parameswaran Nair ,  Alfredo Capretta

IPC分类号： C12N15/115 ,  C12Q1/28

CPC分类号： C12N15/115 ,  C12Q1/28 ,  C12N2310/16

摘要： This disclosure relates to DNA aptamers that bind eosinophil peroxidase, including uses thereof, such as in eosinophil peroxidase detection assays. Also provided is a method for detecting the presence of eosinophil peroxidase in a sample, using the DNA aptamers that bind eosinophil peroxidase. Further provided is a kit for detecting the presence of eosinophil peroxidase in a sample, using the DNA aptamers that bind eosinophil peroxidase.

公开(公告)号：US11549945B2

公开(公告)日：2023-01-10

申请号：US16875707

申请日：2020-05-15

申请人： McMaster University

发明人： John D. Brennan ,  Yingfu Li ,  Monsur Ali ,  Carlos Filipe ,  Michael Gregory Wolfe

IPC分类号： C12Q1/6816 ,  G01N33/569 ,  G01N33/543

摘要： Provided herein are nucleic acid-cleaving catalytic nucleic acid probes, biosensors and lateral flow biosensor devices and methods and kits of using the probes, biosensors and lateral flow biosensor devices for detecting an analyte present on or generated from a microorganism in a test sample, including Helicobacter pylori and methods for determining whether a subject has a Helicobacter pylori infection.

公开(公告)号：US10150986B2

公开(公告)日：2018-12-11

申请号：US15079418

申请日：2016-03-24

申请人： McMaster University

发明人： John D. Brennan ,  Yingfu Li ,  Carmen Carrasquilla

IPC分类号： C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/53 ,  C12Q1/6816

摘要： The present application relates to biosensors, for example comprising an high-molecular weight, tandem repeating, structure-switching nucleic acid aptamers (concatemeric aptamers) to rapidly create patterned sensors via, for example, inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and reporter signalling to provide both qualitative and quantitative detection of one or more analytes. In certain embodiments, inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on-demand printing of sensors even in remote locations.

公开(公告)号：US20190178880A1

公开(公告)日：2019-06-13

申请号：US16274616

申请日：2019-02-13

申请人： McMaster University

发明人： Carlos Filipe ,  John D. Brennan ,  Robert Pelton ,  Sana Jahanshahi-Anbuhi ,  Yingfu Li

IPC分类号： G01N33/543 ,  G01N33/548 ,  C12Q1/686 ,  G01N21/78 ,  C12Q1/66

摘要： The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

公开(公告)号：US10190154B2

公开(公告)日：2019-01-29

申请号：US15091778

申请日：2016-04-06

申请人： McMaster University

发明人： Yingfu Li ,  John D. Brennan ,  Meng Liu

IPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/682 ,  C12Q1/6823 ,  G01N33/53

摘要： The present application discloses a biosensor that comprises a nucleic acid probe absorbed on reduced graphene oxide, the nucleic acid probe comprising an RCA primer sequence linked to a recognition moiety for an analyte to be detected by the biosensor.

公开(公告)号：US11067569B2

公开(公告)日：2021-07-20

申请号：US16274616

申请日：2019-02-13

申请人： McMaster University

发明人： Carlos Filipe ,  John D. Brennan ,  Robert Pelton ,  Sana Jahanshahi-Anbuhi ,  Yingfu Li

IPC分类号： G01N33/543 ,  G01N21/78 ,  G01N33/548 ,  C12Q1/66 ,  C12Q1/686 ,  G01N21/77

摘要： The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

公开(公告)号：US10241109B2

公开(公告)日：2019-03-26

申请号：US15034914

申请日：2014-11-10

申请人： McMaster University

发明人： Carlos Filipe ,  John D. Brennan ,  Robert Pelton ,  Sana Jahanshahi-Anbuhi ,  Yingfu Li

IPC分类号： G01N33/543 ,  G01N33/548 ,  G01N21/78 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/686 ,  G01N21/77

摘要： The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

公开(公告)号：US20190071714A1

公开(公告)日：2019-03-07

申请号：US16121120

申请日：2018-09-04

申请人： McMaster University

发明人： Yingfu Li ,  John D. Brennan ,  Meng Liu

IPC分类号： C12Q1/689 ,  C12N15/10

摘要： Provided herein are aptamers, aptamer probes and biosensor systems that detect C. difficile glutamate dehydrogenase (GDH). Also provided are methods of detecting C. difficile GDH using the aptamers, probes and biosensors and methods of determining whether a subject has a C. difficile infection.

公开(公告)号：US20160281145A1

公开(公告)日：2016-09-29

申请号：US15079418

申请日：2016-03-24

申请人： McMaster University

发明人： John D. Brennan ,  Yingfu Li ,  Carmen Carrasquilla

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/5308 ,  C12Q2525/205 ,  C12Q2565/518 ,  C12Q2565/607

摘要： The present application relates to biosensors, for example comprising an high-molecular weight, tandem repeating, structure-switching nucleic acid aptamers (concatemeric aptamers) to rapidly create patterned sensors via, for example, inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and reporter signalling to provide both qualitative and quantitative detection of one or more analytes. In certain embodiments, inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on-demand printing of sensors even in remote locations.

摘要翻译： 本申请涉及生物传感器，例如包括通过例如喷墨印刷快速形成图案化传感器的高分子量串联重复结构转换核酸适配体（并用适体）。 这些连接适体报告者通过强吸附保持固定在印刷点，但保留足够的分段迁移率进行结构切换和报告信号，以提供一种或多种分析物的定性和定量检测。 在某些实施例中，喷墨打印允许通过多路检测对内部参考的传感器进行图案化，并且为即使在偏远位置的传感器的按需打印提供通用平台。