公开(公告)号：US20060193897A1

公开(公告)日：2006-08-31

申请号：US11198426

申请日：2005-08-05

申请人： Michael Bedford ,  Andrew Morgan ,  Timothy Fowler ,  Kathleen Clarkson ,  Michael Ward ,  Katherine Collier ,  Edmund Larenas

发明人： Michael Bedford ,  Andrew Morgan ,  Timothy Fowler ,  Kathleen Clarkson ,  Michael Ward ,  Katherine Collier ,  Edmund Larenas

IPC分类号： C12N9/42 ,  A23K1/165 ,  C12N1/16

CPC分类号： C12Y302/01004 ,  A23K20/189 ,  A23K50/75 ,  C12N9/2437 ,  C12Y302/01091

摘要： The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase.

摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是EGI，EGII，EGIII及其任何的任何功能活性衍生物中的一种或多种。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。

公开(公告)号：US20060134091A1

公开(公告)日：2006-06-22

申请号：US11351358

申请日：2006-02-10

申请人： Juha Apajalahti ,  Nina Rautonen ,  Michael Bedford

发明人： Juha Apajalahti ,  Nina Rautonen ,  Michael Bedford

IPC分类号： A61K38/47 ,  A23K1/165

CPC分类号： A61K45/06 ,  A23K20/142 ,  A23K20/189 ,  A61K31/205 ,  A61K38/482 ,  Y02A50/473 ,  Y02A50/481 ,  Y10S426/805 ,  Y10S426/807 ,  A61K2300/00

摘要： The present invention relates to the use of a combination of a protease and an inner salt of a quaternary amine carboxylic acid for the preparation of an agent for the treatment and/or prophylaxis of coccidiosis and bacterial infections such as necrotic enterities in animals. Preferred embodiments of the invention comprise the inclusion of a xylanase and/or an α-amylase in the agent. A nutritional additive comprising a combination of an inner salt of a quaternary amine carboxylic acid protease and (optionally) a xylanase and an α-amylase is also disclosed. It can be used for improving the rate of weight gain of animals.

公开(公告)号：US20050208178A1

公开(公告)日：2005-09-22

申请号：US11018645

申请日：2004-12-20

申请人： Michael Bauer ,  Michael Bedford ,  Derrick Pulliam

发明人： Michael Bauer ,  Michael Bedford ,  Derrick Pulliam

IPC分类号： A23B7/10 ,  A23K1/00 ,  A23K1/165 ,  A23K1/18 ,  A23L1/305 ,  C07H21/04 ,  C12N1/18 ,  C12N9/24 ,  C12P21/06 ,  C12Q1/68

CPC分类号： C12N9/2482 ,  A23K20/189 ,  A23K20/20 ,  A23K40/20 ,  A23K40/25 ,  A23K50/10 ,  A23K50/20 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  A23L33/195 ,  C07K2319/02 ,  C12N9/248 ,  C12Y302/01008 ,  Y02A40/818

摘要： The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

摘要翻译： 本发明涉及密码子优化的木聚糖酶编码序列和微生物和酵母中木聚糖酶的表达。 本发明还涉及使用木聚糖酶表达构建体的多个拷贝用于高水平的蛋白质表达。 本发明还涉及木聚糖酶作为饲料或食品添加剂的用途。 本发明还涉及通过在与没有糖基化的相同酶的表达相比糖蛋白的生物体中表达它们来增加耐热性来增加耐热性的方法。 此外，本发明涉及制备饲料，酶饲料添加剂的方法以及减少饲料转化率或增加动物体重增加的方法。

公开(公告)号：US20060083731A1

公开(公告)日：2006-04-20

申请号：US11289364

申请日：2005-11-30

申请人： Michael Bedford

发明人： Michael Bedford

IPC分类号： A61K38/47 ,  A61K36/899

CPC分类号： A61K38/47 ,  A23K20/189 ,  Y02A50/47 ,  Y02A50/481

摘要： Xylanase or a cellulase for the manufacture of an agent for the treatment and/or prophylaxis of bacterial infection in an animal caused by Salmonella, Campylobacter or Clostridium perfringens. It is preferred that xylanase is used in combination with wheat to form an animal feed. Such a diet is particularly effective in controlling Campylobacter and Salmonella in chickens. The use provided by the present invention affords an alternative to antibiotics when controlling bacterial infection in animals. This leads to considerable health, environmental and economic benefits.

摘要翻译： 木聚糖酶或纤维素酶用于制造用于治疗和/或预防由沙门氏菌，弯曲杆菌或产气荚膜梭菌引起的动物细菌感染的试剂。 优选将木聚糖酶与小麦组合使用以形成动物饲料。 这样的饮食在控制鸡中的弯曲杆菌和沙门氏菌方面特别有效。 当控制动物细菌感染时，本发明提供的用途提供了抗生素的替代方案。 这导致了相当大的健康，环境和经济效益。