-
1.发明授权Diagnostic methods for conditions associated with elevated cellular levels of telomerase activity 失效与端粒酶活性升高的细胞水平相关的病症的诊断方法公开(公告)号：US06551774B1
公开(公告)日：2003-04-22
申请号：US09378535
申请日：1999-08-20
申请人： Michael D. West , Calvin B. Harley , Scott L. Weinrich , Catherine M. Strahl , Michael J. McEachern , Jerry Shay , Woodring E. Wright , Elizabeth H. Blackburn , Nam Woo Kim , Homayoun Vaziri
发明人： Michael D. West , Calvin B. Harley , Scott L. Weinrich , Catherine M. Strahl , Michael J. McEachern , Jerry Shay , Woodring E. Wright , Elizabeth H. Blackburn , Nam Woo Kim , Homayoun Vaziri
IPC分类号： C12Q168
CPC分类号： C12N15/1137 , A61K31/70 , A61K38/00 , C12N5/0018 , C12N5/163 , C12N9/1241 , C12N15/10 , C12N15/113 , C12N2501/70 , C12N2510/04 , C12Q1/686 , C12Q1/6886 , C12Q1/703 , C12Q2600/136 , C12Q2600/156 , C12Y207/07049 , C12Q2525/151 , C12Q2521/113
摘要： Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to increase or decrease telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing or reversing the loss of telomeric repeats in aging cells.
摘要翻译： 提供了用于测定端粒长度和端粒酶活性的方法和组合物，以及在增殖性疾病的治疗中增加或减少端粒酶活性的能力。 特别地，引物在使来自其它基因组序列的干扰最小化的条件下伸长，以获得端粒长度或端粒酶活性的准确测定。 此外，提供组合物用于细胞内抑制端粒酶活性，并且显示了用于减缓或逆转衰老细胞中端粒重复的丧失的手段。
-
公开(公告)号：US6007989A
公开(公告)日：1999-12-28
申请号：US819867
申请日：1997-03-14
申请人： Michael D. West , Calvin B. Harley , Scott L. Weinrich , Catherine M. Strahl , Michael J. McEachern , Jerry Shay , Woodring E. Wright , Elizabeth H. Blackburn , Nam Woo Kim , Homayoun Vaziri
发明人： Michael D. West , Calvin B. Harley , Scott L. Weinrich , Catherine M. Strahl , Michael J. McEachern , Jerry Shay , Woodring E. Wright , Elizabeth H. Blackburn , Nam Woo Kim , Homayoun Vaziri
IPC分类号： A61K38/00 , C12N5/00 , C12N5/16 , C12N9/12 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12N15/1137 , A61K31/70 , C12N15/10 , C12N15/113 , C12N5/0018 , C12N5/163 , C12N9/1241 , C12Q1/686 , C12Q1/6886 , C12Q1/703 , C12Y207/07049 , A61K38/00 , C12N2501/70 , C12N2510/04 , C12Q2600/136 , C12Q2600/156
摘要： Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to increase or decrease telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing or reversing the loss of telomeric repeats in aging cells.
-
3.发明授权Therapy and diagnosis of conditions related to telomere length and/or telomerase activity 失效治疗和诊断与端粒长度和/或端粒酶活性有关的病症
公开(公告)号：US5645986A
公开(公告)日：1997-07-08
申请号：US153051
申请日：1993-11-12
申请人： Michael D. West , Calvin B. Harley , Catherine M. Strahl , Michael J. McEachern , Jerry Shay , Woodring E. Wright , Elizabeth H. Blackburn , Homayoun Vaziri
发明人： Michael D. West , Calvin B. Harley , Catherine M. Strahl , Michael J. McEachern , Jerry Shay , Woodring E. Wright , Elizabeth H. Blackburn , Homayoun Vaziri
IPC分类号： A61K38/00 , C12N5/00 , C12N5/16 , C12N9/12 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： A61K31/70 , C12N15/10 , C12N15/113 , C12N15/1137 , C12N5/0018 , C12N5/163 , C12N9/1241 , C12Q1/6827 , C12Q1/686 , C12Q1/6876 , C12Q1/6886 , C12Q1/703 , C12Y207/07049 , A61K38/00 , C12N2501/70 , C12N2510/04 , C12Q2600/136
摘要： Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to increase or decrease telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing or reversing the loss of telomeric repeats in aging cells.
摘要翻译： 提供了用于测定端粒长度和端粒酶活性的方法和组合物，以及在增殖性疾病的治疗中增加或减少端粒酶活性的能力。 特别地，引物在使来自其它基因组序列的干扰最小化的条件下伸长，以获得端粒长度或端粒酶活性的准确测定。 此外，提供组合物用于细胞内抑制端粒酶活性，并且显示了用于减缓或逆转衰老细胞中端粒重复的丧失的手段。
-
公开(公告)号：US08546074B2
公开(公告)日：2013-10-01
申请号：US13523412
申请日：2012-06-14
IPC分类号： C12Q1/00 , G01N33/53 , G01N33/567
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： Methods for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound are described.
摘要翻译： 描述了通过向化合物提供包含转录因子Sir2和Sir2辅因子的测试混合物并在化合物存在下评价测试混合物的组分的活性来筛选化合物的方法。
-
公开(公告)号：US20100105036A1
公开(公告)日：2010-04-29
申请号：US12408575
申请日：2009-03-20
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： This invention relates to methods of screening compounds that modulate cellular and organismal processes by modification of the activity of SIR2 and/or transcription factors, e.g., p53, particularly methods of screening for compounds that modify lifespan and/or metabolism of a cell or an organism by modulation of the activity of SIR2 and/or transcription factors, e.g., p53, and more particularly to methods of screening for compounds that modulate the activity of Sir2 and/or transcription factors, e.g., p53. In particular, the present invention relates to a method for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound. The invention further relates to therapeutic uses of said compounds. The invention further relates to a method of modifying the acetylation status of a transcription factor binding site on histone or DNA by raising local concentrations of Sir2.
摘要翻译： 本发明涉及通过修饰SIR2和/或转录因子活性（例如p53）来筛选调节细胞和生物体过程的化合物的方法，特别是筛选修饰细胞或生物体的寿命和/或代谢的化合物的方法 通过调节SIR2和/或转录因子例如p53的活性，更具体地涉及筛选调节Sir2和/或转录因子例如p53的活性的化合物的方法。 特别地，本发明涉及通过提供包含转录因子Sir2和Sir2辅因子与该化合物的测试混合物来筛选化合物的方法，并且在存在下评价测试混合物的组分的活性 化合物。 本发明还涉及所述化合物的治疗用途。 本发明还涉及通过提高Sir2的局部浓度来改变组蛋白或DNA上的转录因子结合位点的乙酰化状态的方法。
-
公开(公告)号：US20060252076A1
公开(公告)日：2006-11-09
申请号：US11404146
申请日：2006-04-13
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： This invention relates to methods of screening compounds that modulate cellular and organismal processes by modification of the activity of SIR2 and/or transcription factors, e.g., p53, particularly methods of screening for compounds that modify lifespan and/or metabolism of a cell or an organism by modulation of the activity of SIR2 and/or transcription factors, e.g., p53, and more particularly to methods of screening for compounds that modulate the activity of Sir2 and/or transcription factors, e.g., p53. In particular, the present invention relates to a method for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound. The invention further relates to therapeutic uses of said compounds. The invention further relates to a method of modifying the acetylation status of a transcription factor binding site on histone or DNA by raising local concentrations of Sir2.
-
公开(公告)号：US20130071378A1
公开(公告)日：2013-03-21
申请号：US13523412
申请日：2012-06-14
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： Methods for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound are described.
摘要翻译： 描述了通过向化合物提供包含转录因子Sir2和Sir2辅因子的测试混合物并在化合物存在下评价测试混合物的组分的活性来筛选化合物的方法。
-
公开(公告)号：US07572575B2
公开(公告)日：2009-08-11
申请号：US11404146
申请日：2006-04-13
IPC分类号： C12Q1/00 , G01N33/53 , G01N33/567
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： This invention relates to methods of screening compounds that modulate cellular and organismal processes by modification of the activity of SIR2 and/or transcription factors, e.g., p53, particularly methods of screening for compounds that modify lifespan and/or metabolism of a cell or an organism by modulation of the activity of SIR2 and/or transcription factors, e.g., p53, and more particularly to methods of screening for compounds that modulate the activity of Sir2 and/or transcription factors, e.g., p53. In particular, the present invention relates to a method for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound. The invention further relates to therapeutic uses of said compounds. The invention further relates to a method of modifying the acetylation status of a transcription factor binding site on histone or DNA by raising local concentrations of Sir2.
摘要翻译： 本发明涉及通过修饰SIR2和/或转录因子（例如p53）的活性来调节细胞和生物体过程的化合物的方法，特别是筛选修饰细胞或生物体的寿命和/或代谢的化合物的方法 通过调节SIR2和/或转录因子例如p53的活性，更具体地涉及筛选调节Sir2和/或转录因子例如p53的活性的化合物的方法。 特别地，本发明涉及通过提供包含转录因子Sir2和Sir2辅因子与该化合物的测试混合物来筛选化合物的方法，并且在存在下评价测试混合物的组分的活性 化合物。 本发明还涉及所述化合物的治疗用途。 本发明还涉及通过提高Sir2的局部浓度来改变组蛋白或DNA上的转录因子结合位点的乙酰化状态的方法。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.049 秒)
