公开(公告)号：US6007989A

公开(公告)日：1999-12-28

申请号：US819867

申请日：1997-03-14

申请人： Michael D. West ,  Calvin B. Harley ,  Scott L. Weinrich ,  Catherine M. Strahl ,  Michael J. McEachern ,  Jerry Shay ,  Woodring E. Wright ,  Elizabeth H. Blackburn ,  Nam Woo Kim ,  Homayoun Vaziri

发明人： Michael D. West ,  Calvin B. Harley ,  Scott L. Weinrich ,  Catherine M. Strahl ,  Michael J. McEachern ,  Jerry Shay ,  Woodring E. Wright ,  Elizabeth H. Blackburn ,  Nam Woo Kim ,  Homayoun Vaziri

IPC分类号： A61K38/00 ,  C12N5/00 ,  C12N5/16 ,  C12N9/12 ,  C12N15/10 ,  C12N15/113 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34

CPC分类号： C12N15/1137 ,  A61K31/70 ,  C12N15/10 ,  C12N15/113 ,  C12N5/0018 ,  C12N5/163 ,  C12N9/1241 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q1/703 ,  C12Y207/07049 ,  A61K38/00 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q2600/136 ,  C12Q2600/156

摘要： Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to increase or decrease telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing or reversing the loss of telomeric repeats in aging cells.

公开(公告)号：US06551774B1

公开(公告)日：2003-04-22

申请号：US09378535

申请日：1999-08-20

申请人： Michael D. West ,  Calvin B. Harley ,  Scott L. Weinrich ,  Catherine M. Strahl ,  Michael J. McEachern ,  Jerry Shay ,  Woodring E. Wright ,  Elizabeth H. Blackburn ,  Nam Woo Kim ,  Homayoun Vaziri

发明人： Michael D. West ,  Calvin B. Harley ,  Scott L. Weinrich ,  Catherine M. Strahl ,  Michael J. McEachern ,  Jerry Shay ,  Woodring E. Wright ,  Elizabeth H. Blackburn ,  Nam Woo Kim ,  Homayoun Vaziri

IPC分类号： C12Q168

CPC分类号： C12N15/1137 ,  A61K31/70 ,  A61K38/00 ,  C12N5/0018 ,  C12N5/163 ,  C12N9/1241 ,  C12N15/10 ,  C12N15/113 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q1/703 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Y207/07049 ,  C12Q2525/151 ,  C12Q2521/113

摘要： Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to increase or decrease telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing or reversing the loss of telomeric repeats in aging cells.

摘要翻译： 提供了用于测定端粒长度和端粒酶活性的方法和组合物，以及在增殖性疾病的治疗中增加或减少端粒酶活性的能力。 特别地，引物在使来自其它基因组序列的干扰最小化的条件下伸长，以获得端粒长度或端粒酶活性的准确测定。 此外，提供组合物用于细胞内抑制端粒酶活性，并且显示了用于减缓或逆转衰老细胞中端粒重复的丧失的手段。

公开(公告)号：US5989807A

公开(公告)日：1999-11-23

申请号：US255774

申请日：1994-06-07

申请人： Michael D. West ,  Jerry Shay ,  Woodring E. Wright ,  Nam Woo Kim ,  Calvin B. Harley ,  Scott L. Weinrich

发明人： Michael D. West ,  Jerry Shay ,  Woodring E. Wright ,  Nam Woo Kim ,  Calvin B. Harley ,  Scott L. Weinrich

IPC分类号： A61K38/00 ,  C12N5/00 ,  C12N9/12 ,  C12N15/10 ,  C12N15/113 ,  C12Q1/68 ,  C12Q1/70 ,  C07H21/04 ,  C12P19/34

CPC分类号： A61K31/70 ,  C12N15/10 ,  C12N15/113 ,  C12N15/1137 ,  C12N5/0018 ,  C12N9/1241 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Y207/07049 ,  A61K38/00 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q2521/113

摘要： Methods and kits are provided for diagnosis of specific cancerous conditions. The invention features a method for diagnosis of a condition in a patient associated with an elevated level of telomerase activity within a cell. The method involves determining the presence or amount of telomerase within the cells in the patient, e.g. by the use of the polymerase chain reaction. In one embodiment of the invention, the condition associated with elevated telomerase activity is chosen from prostate cancer, breast cancer, colon cancer, renal cancer, ovarian/cervical cancer, lung cancer, and leukemia. The invention allows cancer to be detected even in tissues and cells which are not positive by pathology.

公开(公告)号：US06391554B1

公开(公告)日：2002-05-21

申请号：US09447151

申请日：1999-11-23

申请人： Michael D. West ,  Jerry W. Shay ,  Woodring E. Wright ,  Nam Woo Kim ,  Calvin B. Harley ,  Scott L. Weinrich

发明人： Michael D. West ,  Jerry W. Shay ,  Woodring E. Wright ,  Nam Woo Kim ,  Calvin B. Harley ,  Scott L. Weinrich

IPC分类号： C12Q168

CPC分类号： A61K31/70 ,  A61K38/00 ,  C12N5/0018 ,  C12N9/1241 ,  C12N15/10 ,  C12N15/113 ,  C12N15/1137 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2521/113 ,  C12Y207/07049

摘要： Methods and kits are provided for diagnosis of specific cancerous conditions. The invention features a method for diagnosis of a condition in a patient associated with an elevated level of telomerase activity within a cell. The method involves determining the presence or amount of telomerase within the cells in the patient, e.g. by the use of the polymerase chain reaction. In one embodiment of the invention, the condition associated with elevated telomerase activity is chosen from prostate cancer, breast cancer, colon cancer, renal cancer, ovarian/cervical cancer, lung cancer, and leukemia. The invention allows cancer to be detected even in tissues and cells which are not positive by pathology.

摘要翻译： 提供方法和试剂盒用于诊断特定的癌症。 本发明的特征在于用于诊断与细胞内端粒酶活性水平升高相关的患者病情的方法。 该方法涉及确定患者细胞内端粒酶的存在或数量，例如， 通过使用聚合酶链反应。 在本发明的一个实施方案中，与端粒酶活性升高相关的病症选自前列腺癌，乳腺癌，结肠癌，肾癌，卵巢/子宫颈癌，肺癌和白血病。 本发明允许在病理学上不是阳性的组织和细胞中检测到癌症。

公开(公告)号：US5846723A

公开(公告)日：1998-12-08

申请号：US770565

申请日：1996-12-20

申请人： Nam Woo Kim ,  Fred Wu ,  James T. Kealey ,  Ronald Pruzan ,  Scott L. Weinrich

发明人： Nam Woo Kim ,  Fred Wu ,  James T. Kealey ,  Ronald Pruzan ,  Scott L. Weinrich

IPC分类号： A61K38/00 ,  C12N15/113 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12N15/1137 ,  C12Y207/07049 ,  A61K38/00 ,  C12N2310/321

摘要： Methods of detecting the RNA component of telomerase, diagnosing cancer, and determining its prognosis using polynucleotides that hybridize to the RNA component of mammalian telomerase in a sample.

摘要翻译： 使用与样品中哺乳动物端粒酶的RNA组分杂交的多核苷酸检测端粒酶的RNA组分，诊断癌症并确定其预后的方法。

公开(公告)号：US5837453A

公开(公告)日：1998-11-17

申请号：US482132

申请日：1995-06-07

申请人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

发明人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

IPC分类号： A61K38/00 ,  C12N5/00 ,  C12N9/12 ,  C12N15/10 ,  C12N15/113 ,  C12Q1/48 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/574 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC分类号： A61K31/70 ,  C12N15/10 ,  C12N15/113 ,  C12N15/1137 ,  C12N5/0018 ,  C12N9/1241 ,  C12Q1/48 ,  C12Q1/6816 ,  C12Y207/07049 ,  G01N33/57496 ,  A61K38/00 ,  C12N2501/70 ,  C12N2510/04 ,  G01N2333/91245

摘要： Telomerase activity in a sample can be measured using a two reaction protocol involving telomerase substrate and primer extension steps. The presence of telomerase activity in a human somatic tissue or cell sample is positively correlated with the presence of cancer and can be used to diagnose the course of disease progression in a patient.

摘要翻译： 样品中的端粒酶活性可以使用涉及端粒酶底物和引物延伸步骤的两个反应方案来测量。 人体细胞组织或细胞样品中端粒酶活性的存在与癌症的存在呈正相关，可用于诊断患者疾病进展的过程。

公开(公告)号：US5804380A

公开(公告)日：1998-09-08

申请号：US632662

申请日：1996-04-15

申请人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

发明人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

IPC分类号： A61K38/00 ,  C12N5/00 ,  C12N9/12 ,  C12N15/10 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12N5/0018 ,  A61K31/70 ,  C12N15/10 ,  C12N15/113 ,  C12N15/1137 ,  C12N9/1241 ,  C12Q1/48 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Y207/07049 ,  G01N33/57496 ,  A61K38/00 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q2600/136 ,  G01N2333/91245 ,  Y10S435/81

摘要： Telomerase activity in a sample can be measured using a two reaction protocol. The first reaction involves the formation of telomerase substrate extension products from a telomerase substrate. The second reaction involves replication of the telomerase substrate extension products and/or amplification of signal generated by a bound probe. The presence of telomerase activity in a human somatic tissue or cell sample is positively correlated with the presence of cancer and can be used to diagnose a disease or other conditions of medical interest, as well as the course of disease progression or remission in a patient.

公开(公告)号：US5891639A

公开(公告)日：1999-04-06

申请号：US100153

申请日：1998-06-18

申请人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

发明人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

IPC分类号： A61K38/00 ,  C12N5/00 ,  C12N9/12 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/574

CPC分类号： C12Q1/6886 ,  A61K31/70 ,  C12N15/10 ,  C12N15/113 ,  C12N15/1137 ,  C12N5/0018 ,  C12N9/1241 ,  C12Q1/48 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Y207/07049 ,  G01N33/57496 ,  A61K38/00 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q2600/136 ,  G01N2333/91245 ,  Y10S435/81

摘要： Telomerase activity in a sample can be measured using a two reaction protocol. The first reaction involves the formation of telomerase substrate extension products from a telomerase substrate. The second reaction involves replication of the telomerase substrate extension products and/or amplification of signal generated by a bound probe. The presence of telomerase activity in a human somatic tissue or cell sample is positively correlated with the presence of cancer and can be used to diagnose a disease or other conditions of medical interest, as well as the course of disease progression or remission in a patient.

公开(公告)号：US6054314A

公开(公告)日：2000-04-25

申请号：US926648

申请日：1997-09-10

申请人： Nam Woo Kim

发明人： Nam Woo Kim

IPC分类号： C12N5/00 ,  C12N5/06 ,  C12Q1/34 ,  C12Q1/68

CPC分类号： C12Q1/34 ,  C12Q1/6886 ,  C12Q2600/158

摘要： A substantially non-invasive and efficient method for collecting cells from the internal organs of a subject is provided. In one embodiment, energy from an external energy source is applied to the subject that is sufficient to loosen the cells from an internal cellular surface of an internal organ so that at least a portion of the loosened cells are detached from the internal cellular surface or the organ. The detached cells are collected from the subject and can be analyzed for a disease state. The methods described herein provide methods for detecting of disease states before macroscopic evidence of the disease state that also facilitate inexpensive mass screening.

摘要翻译： 提供了从受试者的内部器官收集细胞的基本上非侵入性和有效的方法。 在一个实施例中，来自外部能量源的能量被施加到受试者，其足以从内部器官的内部细胞表面松开细胞，使得至少一部分松动细胞从内部细胞表面或 器官。 从受试者收集分离的细胞，并且可以分析疾病状态。 本文描述的方法提供了在疾病状态的宏观证据之前检测疾病状态的方法，其也便于便宜的大规模筛查。

公开(公告)号：US5863726A

公开(公告)日：1999-01-26

申请号：US631554

申请日：1996-04-12

申请人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

发明人： Calvin Bruce Harley ,  Nam Woo Kim ,  Scott Lawrence Weinrich

IPC分类号： A61K38/00 ,  C12N5/00 ,  C12N9/12 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/574 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  A61K31/70 ,  C12N15/10 ,  C12N15/113 ,  C12N15/1137 ,  C12N5/0018 ,  C12N9/1241 ,  C12Q1/48 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Y207/07049 ,  G01N33/57496 ,  A61K38/00 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q2600/136 ,  G01N2333/91245 ,  Y10S435/81

摘要： Telomerase activity in a sample can be measured using a two reaction protocol. The first reaction involves the formation of telomerase substrate extension products from a telomerase substrate. The second reaction involves replication of the telomerase substrate extension products and/or amplification of signal generated by a bound probe. The presence of telomerase activity in a human somatic tissue or cell sample is positively correlated with the presence of cancer and can be used to diagnose a disease or other conditions of medical interest, as well as the course of disease progression or remission in a patient.

摘要翻译： 样品中的端粒酶活性可以使用两种反应方案测定。 第一反应涉及从端粒酶底物形成端粒酶底物延伸产物。 第二个反应涉及端粒酶底物延伸产物的复制和/或由结合的探针产生的信号的放大。 人体细胞组织或细胞样品中端粒酶活性的存在与癌症的存在呈正相关，可用于诊断疾病或其他医疗感兴趣的病症，以及患者疾病进展或缓解过程。