公开(公告)号：US6018104A

公开(公告)日：2000-01-25

申请号：US459595

申请日：1995-06-02

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

IPC分类号： A01H5/00 ,  A01H5/10 ,  C07H21/04 ,  C07K14/325 ,  C12N5/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/09 ,  C12N15/11 ,  C12N15/32 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12N15/87

CPC分类号： C07K14/325 ,  C12N15/8286 ,  C12N9/12 ,  C12N9/88

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

摘要翻译： 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子，特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。

公开(公告)号：US6075185A

公开(公告)日：2000-06-13

申请号：US459504

申请日：1995-06-02

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

IPC分类号： A01H5/00 ,  A01H5/10 ,  C07H21/04 ,  C07K14/325 ,  C12N5/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/09 ,  C12N15/11 ,  C12N15/32 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12N15/87

CPC分类号： C07K14/325 ,  C12N15/8286 ,  C12N9/12 ,  C12N9/88

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

公开(公告)号：US5625136A

公开(公告)日：1997-04-29

申请号：US951715

申请日：1992-09-25

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

IPC分类号： A01H5/00 ,  A01H5/10 ,  C07H21/04 ,  C07K14/325 ,  C12N5/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/09 ,  C12N15/11 ,  C12N15/32 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12N15/87

CPC分类号： C07K14/325 ,  C12N15/8286 ,  C12N9/12 ,  C12N9/88

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

摘要翻译： 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子，特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。

公开(公告)号：US06403865B1

公开(公告)日：2002-06-11

申请号：US08438666

申请日：1995-05-10

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James O. Linder ,  Robert L. Miller ,  Bruce W. Skillings ,  Alan W. Mousel ,  Albert R. Hornbrook ,  Christopher P. Clucas ,  Moez Rajabali Meghji ,  Andreas H. Tanner ,  Francis E. Cassagne ,  Gilles Pollini ,  Terry Ray Colbert ,  Francis P. Cammack

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James O. Linder ,  Robert L. Miller ,  Bruce W. Skillings ,  Alan W. Mousel ,  Albert R. Hornbrook ,  Christopher P. Clucas ,  Moez Rajabali Meghji ,  Andreas H. Tanner ,  Francis E. Cassagne ,  Gilles Pollini ,  Terry Ray Colbert ,  Francis P. Cammack

IPC分类号： A01H500

CPC分类号： C12N9/12 ,  C07K14/325 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.

摘要翻译： 公开了用感兴趣的核酸序列转化玉米的方法。 该方法包括使未成熟的合子胚或I型愈伤组织进行高速微粒轰击。 该方法能够生产具有商业重要性的转化玉米品系及其杂交组合。

公开(公告)号：US06320100B1

公开(公告)日：2001-11-20

申请号：US09547422

申请日：2000-04-11

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

IPC分类号： A01H500

CPC分类号： C07K14/325 ,  C12N9/12 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

摘要翻译： 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子，特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。

公开(公告)号：US5859336A

公开(公告)日：1999-01-12

申请号：US459448

申请日：1995-06-02

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

IPC分类号： A01H5/00 ,  A01H5/10 ,  C07H21/04 ,  C07K14/325 ,  C12N5/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/09 ,  C12N15/11 ,  C12N15/32 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12N15/87 ,  C12N15/63

CPC分类号： C07K14/325 ,  C12N15/8286 ,  C12N9/12 ,  C12N9/88

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

摘要翻译： 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子，特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。

公开(公告)号：US6051760A

公开(公告)日：2000-04-18

申请号：US460408

申请日：1995-06-02

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Gregory W. Warren ,  Stephen V. Evola ,  Martha S. Wright ,  Karen L. Launis ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie

IPC分类号： A01H5/00 ,  A01H5/10 ,  C07H21/04 ,  C07K14/325 ,  C12N5/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/09 ,  C12N15/11 ,  C12N15/32 ,  C12N15/54 ,  C12N15/60 ,  C12N15/82 ,  C12N15/87

CPC分类号： C07K14/325 ,  C12N15/8286 ,  C12N9/12 ,  C12N9/88

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

公开(公告)号：US4992375A

公开(公告)日：1991-02-12

申请号：US634854

申请日：1984-07-26

申请人： Martha S. Wright

发明人： Martha S. Wright

IPC分类号： C12N5/00 ,  C12N5/04

CPC分类号： C12N5/04 ,  C12N5/0025

摘要： A method for regenerating soybean plants from cotyledonary nodes is disclosed. Soybean seeds are germinated on nutrient medium containing a cytokinin to produce a donor plant. The cotyledonary nodes of the donor plant are excised and divided into pieces. The cotyledonary node pieces are cultured on nutrient medium containing a cytokinin until callus tissue develops which contains shoots. The shoots are removed and rooted on nutrient medium free of exogenous hormone to form a plantlet.

摘要翻译： 公开了一种从子叶节点再生大豆植物的方法。 大豆种子在含有细胞分裂素的营养培养基上发芽以产生供体植物。 将供体植物的子叶节点切除并分成块。 将子叶节点在含有细胞分裂素的营养培养基上培养，直到含有芽的愈伤组织发育。 将芽切除并植根于不含外源激素的营养培养基上以形成小植株。