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公开(公告)号：US07776565B2
公开(公告)日：2010-08-17
申请号：US12394479
申请日：2009-02-27
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译： 提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。 方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。 这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定临界加工酶的基因序列。 组合物包含感兴趣的细胞产生唾液酸化糖蛋白。 所述方法和组合物可用于糖蛋白的异源表达。
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公开(公告)号：US06949372B2
公开(公告)日：2005-09-27
申请号：US09930440
申请日：2001-08-16
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译： 提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。 方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。 这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定关键加工酶的基因序列。 组合物包含感兴趣的细胞产生唾液酸化糖蛋白。 所述方法和组合物可用于糖蛋白的异源表达。
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公开(公告)号：US20090226968A1
公开(公告)日：2009-09-10
申请号：US12394479
申请日：2009-02-27
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译： 提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。 方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。 这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定关键加工酶的基因序列。 组合物包含感兴趣的细胞产生唾液酸化糖蛋白。 所述方法和组合物可用于糖蛋白的异源表达。
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公开(公告)号：US08546105B2
公开(公告)日：2013-10-01
申请号：US12806292
申请日：2010-08-09
IPC分类号： C12N15/09 , C12N15/79 , C12N15/866 , C12P19/30
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
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公开(公告)号：US20110229931A1
公开(公告)日：2011-09-22
申请号：US12806292
申请日：2010-08-09
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译： 提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。 方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。 这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定关键加工酶的基因序列。 组合物包含感兴趣的细胞产生唾液酸化糖蛋白。 所述方法和组合物可用于糖蛋白的异源表达。
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公开(公告)号：US06333182B1
公开(公告)日：2001-12-25
申请号：US09516143
申请日：2000-03-01
IPC分类号： C12N900
CPC分类号： C12N9/1241 , A61K48/00 , C07K2319/00 , C12N9/88 , C12N2799/026
摘要： The present invention relates to novel human glycosylation enzyme polypeptides and isolated nucleic acids containing the coding regions of the genes encoding such polypeptides. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human glycosylation enzyme polypeptides. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human glycosylation enzyme polypeptides.
摘要翻译： 本发明涉及新的人糖基化酶多肽和含有编码这些多肽的基因的编码区的分离的核酸。 还提供了用于产生人糖基化酶多肽的载体，宿主细胞，抗体和重组方法。 本发明还涉及用于诊断和治疗与这些新型人糖基化酶多肽有关的病症的诊断和治疗方法。
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公开(公告)号：US06858415B2
公开(公告)日：2005-02-22
申请号：US10759277
申请日：2004-01-20
CPC分类号： C12N9/1241 , A61K48/00 , C07K2319/00 , C12N9/88 , C12N2799/026
摘要： The present invention relates to novel human glycosylation enzyme polypeptides and isolated nucleic acids containing the coding regions of the genes encoding such polypeptides. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human glycosylation enzyme polypeptides. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human glycosylation enzyme polypeptides.
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公开(公告)号：US06783971B2
公开(公告)日：2004-08-31
申请号：US09984205
申请日：2001-10-29
IPC分类号： C12N988
CPC分类号： C12N9/1241 , A61K48/00 , C07K2319/00 , C12N9/88 , C12N2799/026
摘要： The present invention relates to novel human glycosylation enzyme polypeptides and isolated nucleic acids containing the coding regions of the genes encoding such polypeptides. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human glycosylation enzyme polypeptides. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human glycosylation enzyme polypeptides.
摘要翻译： 本发明涉及新的人糖基化酶多肽和含有编码这些多肽的基因的编码区的分离的核酸。 还提供了用于产生人糖基化酶多肽的载体，宿主细胞，抗体和重组方法。 本发明还涉及用于诊断和治疗与这些新型人糖基化酶多肽有关的病症的诊断和治疗方法。
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9.发明申请METHOD FOR EXTRACTION AND PURIFICATION OF OILS FROM MICROALGAL BIOMASS USING HIGH-PRESSURE CO2 AS A SOLUTE 审中-公开使用高压二氧化碳作为溶剂从微生物生物质中提取和纯化油的方法公开(公告)号：US20140004605A1
公开(公告)日：2014-01-02
申请号：US13816737
申请日：2011-08-16
IPC分类号： C11B1/10
CPC分类号： C11B1/10 , C10G2300/1011 , C10G2300/1014 , C10G2300/4081 , C10L1/02 , C11B3/02 , Y02P30/20
摘要： The present invention provides methods for the isolation of oils from intact or lysed microorganisms in aqueous media with pressurized carbon dioxide as a solute. Such oils may be used for the production of biofuels. Also provided for are methods for harvesting and rupturing whole cell microorganisms in aqueous media with pressurized carbon dioxide as a solute
摘要翻译： 本发明提供了在具有加压二氧化碳作为溶质的水性介质中从完整或裂解的微生物分离油的方法。 这种油可用于生产生物燃料。 还提供了用加压二氧化碳作为溶质在水性介质中收获和破裂全细胞微生物的方法
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10.发明申请METHOD AND COMPOSITION FOR GENERATING PROGRAMMED CELL DEATH RESISTANT ALGAL CELLS 审中-公开用于产生编程性细胞死亡细胞的方法和组合物公开(公告)号：US20130065312A1
公开(公告)日：2013-03-14
申请号：US13505783
申请日：2010-11-03
CPC分类号： C12N15/8263 , A01G33/00 , C07K14/4747 , C12N15/8261 , Y02A40/146 , Y02A40/88
摘要： The present invention provides transgenic algal cells resistant to programmed cell death (PCD) and methods and compositions useful in generating such cells. Specifically, the invention utilizes expression of one or more mammalian anti-apoptotic genes in algal cells to promote resistance to PCD, which is useful for stress tolerance and increased cell viability and biomass production during cultivation.
摘要翻译： 本发明提供对程序性细胞死亡（PCD）有抗性的转基因藻类细胞以及可用于产生这种细胞的方法和组合物。 具体地，本发明利用藻类细胞中一种或多种哺乳动物抗凋亡基因的表达来促进对PCD的抗性，其对于胁迫耐受性和培养期间增加的细胞活力和生物量产生是有用的。
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