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公开(公告)号：US20120075453A1
公开(公告)日：2012-03-29
申请号：US13047002
申请日：2011-03-14
Applicant: Michael Kilpatrick , Triantafyllos Tafas
Inventor： Michael Kilpatrick , Triantafyllos Tafas
CPC classification number: C12Q1/6816 , C12Q1/6841 , G01N33/56966 , G01N33/689 , G01N33/721 , G01N2333/805 , G01N2800/385 , G02B21/367 , C12Q2563/107 , C12Q2537/143
Abstract: A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.
Abstract translation: 提供了一种从细胞样品中检测和定量多种独特荧光信号的方法。 该方法结合了免疫组织化学和荧光标记的原位杂交技术。 该方法可用于鉴定细胞如染色体和蛋白质的特定亚细胞组分。
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2.发明授权Detection of circulating tumor cells in peripheral blood with an automated scanning fluorescence microscope 有权用自动扫描荧光显微镜检测外周血中的循环肿瘤细胞公开(公告)号：US08088715B2
公开(公告)日：2012-01-03
申请号：US12270820
申请日：2008-11-13
Applicant: Sir Walter Bodmer , Triantafyllos Tafas , Michael Kilpatrick , Petros Tsipouras , Xiuzhong Wang , Youngmin Kim , Michael Thomas , Antti Seppo , Triantafyllia Ntouroupi , Changhua Yu , Charles Climer
Inventor： Sir Walter Bodmer , Triantafyllos Tafas , Michael Kilpatrick , Petros Tsipouras , Xiuzhong Wang , Youngmin Kim , Michael Thomas , Antti Seppo , Triantafyllia Ntouroupi , Changhua Yu , Charles Climer
IPC: C40B30/04
CPC classification number: G06K9/00127 , G01N21/6458
Abstract: An automated, highly sensitive, specific and potentially quantitative detection method using an automated microscope for identifying and enumerating rare cancer cells in blood and other fluids.
Abstract translation: 一种使用自动显微镜识别和列举血液和其他液体中稀有癌细胞的自动化，高灵敏度，特异性和潜在的定量检测方法。
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3.发明申请SYSTEM FOR DETECTING INFECTIOUS AGENTS USING COMPUTER-CONTROLLED AUTOMATED IMAGE ANALYSIS 失效使用计算机控制自动图像分析检测感染性代理的系统公开(公告)号：US20110129138A1
公开(公告)日：2011-06-02
申请号：US13022348
申请日：2011-02-07
Applicant: Triantafyllos Tafas , Michael Kilpatrick , Petros Tsipouras
Inventor： Triantafyllos Tafas , Michael Kilpatrick , Petros Tsipouras
IPC: G06K9/00
CPC classification number: G06K9/00147 , C12Q1/04 , G01N15/1475 , G06K9/00127 , G06T7/0012 , G06T2207/30024
Abstract: A method for recognizing an object in an image is disclosed wherein a fractal map of the image is generated by estimating the fractal dimension of each pixel in the image. The fractal map may be segmented by thresholding and locations of candidate objects are determined. The pixel value of the image pixel corresponding to the same location where the candidate object is found in the fractal map may be compared to a threshold value. If the pixel value is greater than the threshold value, the candidate object is recognized as a valid object.
Abstract translation: 公开了一种用于识别图像中的对象的方法，其中通过估计图像中每个像素的分形维数来生成图像的分形图。 可以通过阈值分割分形图并确定候选对象的位置。 可以将对应于在分形图中找到候选对象的相同位置的图像像素的像素值与阈值进行比较。 如果像素值大于阈值，则候选对象被识别为有效对象。
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公开(公告)号：US20110122492A1
公开(公告)日：2011-05-26
申请号：US12833887
申请日：2010-07-09
Applicant: Michael Thomas , Charles Climer , Richard Schlueter , Triantafyllos P. Tafas , Michael Kilpatrick , Antti Seppo
Inventor： Michael Thomas , Charles Climer , Richard Schlueter , Triantafyllos P. Tafas , Michael Kilpatrick , Antti Seppo
IPC: G02B21/34 , B01D35/02 , B01D29/085
CPC classification number: G02B21/34
Abstract: An filter, apparatus and method for preparing microscope slides is presented. After filtration of the specimen is performed, the filter containing the filtrand is configured to be microscopically examined. The filter may either be affixed to a microscope slide or, in an embodiment, the filter may be incorporated into a microscope slide.
Abstract translation: 提出了一种用于制备显微镜载片的过滤器，设备和方法。 过滤样品后，将含有过滤物的过滤器配置为进行显微镜检查。 过滤器可以被固定到显微镜载玻片上，或者在一个实施例中，过滤器可以结合到显微镜载片中。
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公开(公告)号：US20080285837A1
公开(公告)日：2008-11-20
申请号：US11924941
申请日：2007-10-26
Applicant: Michael Kilpatrick , Triantafyllos Tafas
Inventor： Michael Kilpatrick , Triantafyllos Tafas
IPC: G06K9/46
CPC classification number: C12Q1/6816 , C12Q1/6841 , G01N33/56966 , G01N33/689 , G01N33/721 , G01N2333/805 , G01N2800/385 , G02B21/367 , C12Q2563/107 , C12Q2537/143
Abstract: A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.
Abstract translation: 提供了一种从细胞样品中检测和定量多种独特荧光信号的方法。 该方法结合了免疫组织化学和荧光标记的原位杂交技术。 该方法可用于鉴定细胞如染色体和蛋白质的特定亚细胞组分。
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Patent Agency Ranking
6.发明申请METHOD FOR COMBINING IMMUNOSTAINING AND FISH USING COVALENTLY BINDING FLUOROPHORES 有权使用共轭结合荧光素组合免疫和鱼类的方法公开(公告)号：US20070243545A1
公开(公告)日：2007-10-18
申请号：US11685736
申请日：2007-03-13
Applicant: Michael Kilpatrick , Antti Seppo , Triantafyllos Tafas
Inventor： Michael Kilpatrick , Antti Seppo , Triantafyllos Tafas
IPC: C12Q1/68 , G01N33/567 , G06T7/40 , G06F19/00
CPC classification number: G01N33/582 , C12Q1/6813 , C12Q1/6841 , G01N33/56966 , C12Q2543/10 , C12Q2563/131
Abstract: Among embodiments is disclosed a process for immunostaining a sample such that the stain is stable under FISH conditions
Abstract translation: 在实施方案中公开了一种免疫染色样品的方法，使得染色剂在FISH条件下是稳定的
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公开(公告)号：US20070009389A1
公开(公告)日：2007-01-11
申请号：US11177036
申请日：2005-07-08
Applicant: Antti Seppo , Triantafyllos Tafas , Michael Kilpatrick
Inventor： Antti Seppo , Triantafyllos Tafas , Michael Kilpatrick
CPC classification number: G02B21/34 , B01L3/508 , B01L2300/046 , B01L2300/0822 , G01N1/2813
Abstract: A cell deposition chamber and a method for depositing a biological sample on a microscope slide using the chamber. The chamber is removably sealed onto a sample receiving area on the slide and has a sealable opening to allow aspiration of the sample from the slide surface. The chamber is configured to allow the aspiration of all areas of the slide surface by a pipette and the chamber drains fully when inverted. The chamber can be fitted with a disposable liner to make the chamber reusable.
Abstract translation: 一种细胞沉积室和使用该腔室将生物样品沉积在显微镜载玻片上的方法。 腔室可移除地密封在载玻片上的样品接收区域上，并且具有可密封的开口以允许样品从滑动表面抽吸。 腔室被配置为允许通过移液管吸取滑动表面的所有区域，并且当倒置时室被完全排出。 该腔室可以安装一次性衬垫，以使腔室可重复使用。
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8.发明授权Detection of circulating tumor cells in peripheral blood with an automated scanning fluorescence microscope 有权用自动扫描荧光显微镜检测外周血中的循环肿瘤细胞公开(公告)号：US08445411B2
公开(公告)日：2013-05-21
申请号：US13337823
申请日：2011-12-27
Applicant: Sir Walter Bodmer , Triantafyllos P. Tafas , Michael Kilpatrick , Petros Tsipouras , Xiuzhong Wang , Youngmin Kim , Michael Thomas , Antti Seppo , Triantafyllia Ntouroupi , Changhua Yu , Charles Climer
Inventor： Sir Walter Bodmer , Triantafyllos P. Tafas , Michael Kilpatrick , Petros Tsipouras , Xiuzhong Wang , Youngmin Kim , Michael Thomas , Antti Seppo , Triantafyllia Ntouroupi , Changhua Yu , Charles Climer
IPC: C40B30/04
CPC classification number: G06K9/00127 , G01N21/6458
Abstract: An automated, highly sensitive, specific and potentially quantitative detection method using an automated microscope for identifying and enumerating rare cancer cells in blood and other fluids.
Abstract translation: 一种使用自动显微镜识别和列举血液和其他液体中稀有癌细胞的自动化，高灵敏度，特异性和潜在的定量检测方法。
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公开(公告)号：US20130078636A1
公开(公告)日：2013-03-28
申请号：US13677884
申请日：2012-11-15
Applicant: Michael Kilpatrick , Triantafyllos P. Tafas
Inventor： Michael Kilpatrick , Triantafyllos P. Tafas
IPC: C12Q1/68
CPC classification number: C12Q1/6816 , C12Q1/6841 , G01N33/56966 , G01N33/689 , G01N33/721 , G01N2333/805 , G01N2800/385 , G02B21/367 , C12Q2563/107 , C12Q2537/143
Abstract: A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.
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10.发明授权System for detecting infectious agents using computer-controlled automated image analysis 失效使用计算机控制的自动图像分析检测感染因子的系统公开(公告)号：US07991213B2
公开(公告)日：2011-08-02
申请号：US13022348
申请日：2011-02-07
Applicant: Triantafyllos P. Tafas , Michael Kilpatrick , Petros Tsipouras
Inventor： Triantafyllos P. Tafas , Michael Kilpatrick , Petros Tsipouras
CPC classification number: G06K9/00147 , C12Q1/04 , G01N15/1475 , G06K9/00127 , G06T7/0012 , G06T2207/30024
Abstract: A method for recognizing an object in an image is disclosed wherein a fractal map of the image is generated by estimating the fractal dimension of each pixel in the image. The fractal map may be segmented by thresholding and locations of candidate objects are determined. The pixel value of the image pixel corresponding to the same location where the candidate object is found in the fractal map may be compared to a threshold value. If the pixel value is greater than the threshold value, the candidate object is recognized as a valid object.
Abstract translation: 公开了一种用于识别图像中的对象的方法，其中通过估计图像中每个像素的分形维数来生成图像的分形图。 可以通过阈值分割分形图并确定候选对象的位置。 可以将对应于在分形图中找到候选对象的相同位置的图像像素的像素值与阈值进行比较。 如果像素值大于阈值，则候选对象被识别为有效对象。
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