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公开(公告)号:US06720488B2
公开(公告)日:2004-04-13
申请号:US09988462
申请日:2001-11-20
申请人: Michael Koziel , Nalini Desai , Gary M. Pace , Jan Suttie , Nadine Carozzi , Cindy Boyce , John L. Dawson , Erik Dunder , Martha Wright , Karen Launis , Steven J. Rothstein , Kelly Lewis , Gregory Warren , Steve Evola
发明人: Michael Koziel , Nalini Desai , Gary M. Pace , Jan Suttie , Nadine Carozzi , Cindy Boyce , John L. Dawson , Erik Dunder , Martha Wright , Karen Launis , Steven J. Rothstein , Kelly Lewis , Gregory Warren , Steve Evola
IPC分类号: A01H510
CPC分类号: C07K14/325 , C12N9/12 , C12N9/88 , C12N15/8286 , Y02A40/162
摘要: DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.
摘要翻译: 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽,特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子,特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。
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2.
公开(公告)号:US20060117407A1
公开(公告)日:2006-06-01
申请号:US11071896
申请日:2005-03-02
申请人: Michael Koziel , Nalini Desai , Kelly Lewis , Vance Kramer , Gregory Warren , Steve Evola , Lyle Crossland , Martha Wright , Ellis Merlin , Karen Launis , Steven Rothstein , Cindy Boyce , John Dawson , Erik Dunder , Gary Pace , Jan Suttie , Nadine Carozzi , Annick De Framond , James Linder , Robert Miller , Bruce Skillings , Alan Mousel , Albert Hornbrook , Christopher Clucas , Moez Meghji , Andreas Tanner , Francis Cassagne , Gilles Pollini , Terry Colbert , Francis Cammack
发明人: Michael Koziel , Nalini Desai , Kelly Lewis , Vance Kramer , Gregory Warren , Steve Evola , Lyle Crossland , Martha Wright , Ellis Merlin , Karen Launis , Steven Rothstein , Cindy Boyce , John Dawson , Erik Dunder , Gary Pace , Jan Suttie , Nadine Carozzi , Annick De Framond , James Linder , Robert Miller , Bruce Skillings , Alan Mousel , Albert Hornbrook , Christopher Clucas , Moez Meghji , Andreas Tanner , Francis Cassagne , Gilles Pollini , Terry Colbert , Francis Cammack
CPC分类号: C12N9/12 , C07K14/325 , C12N9/88 , C12N15/8286 , Y02A40/162
摘要: Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.
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公开(公告)号:US20060021095A1
公开(公告)日:2006-01-26
申请号:US10755092
申请日:2004-01-08
申请人: Michael Koziel , Nalini Desai , Kelly Lewis , Gregory Warren , Steve Evola , Martha Wright , Karen Launis , Steven Rothstein , Cindy Boyce , John Dawson , Erik Dunder , Gary Pace , Jan Suttie , Nadine Carozzi
发明人: Michael Koziel , Nalini Desai , Kelly Lewis , Gregory Warren , Steve Evola , Martha Wright , Karen Launis , Steven Rothstein , Cindy Boyce , John Dawson , Erik Dunder , Gary Pace , Jan Suttie , Nadine Carozzi
CPC分类号: C07K14/325 , C12N9/12 , C12N9/88 , C12N15/8286 , Y02A40/162
摘要: DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.
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