公开(公告)号：US06720488B2

公开(公告)日：2004-04-13

申请号：US09988462

申请日：2001-11-20

申请人： Michael Koziel ,  Nalini Desai ,  Gary M. Pace ,  Jan Suttie ,  Nadine Carozzi ,  Cindy Boyce ,  John L. Dawson ,  Erik Dunder ,  Martha Wright ,  Karen Launis ,  Steven J. Rothstein ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola

发明人： Michael Koziel ,  Nalini Desai ,  Gary M. Pace ,  Jan Suttie ,  Nadine Carozzi ,  Cindy Boyce ,  John L. Dawson ,  Erik Dunder ,  Martha Wright ,  Karen Launis ,  Steven J. Rothstein ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola

IPC分类号： A01H510

CPC分类号： C07K14/325 ,  C12N9/12 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

摘要翻译： 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子，特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。

公开(公告)号：US20060117407A1

公开(公告)日：2006-06-01

申请号：US11071896

申请日：2005-03-02

申请人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Vance Kramer ,  Gregory Warren ,  Steve Evola ,  Lyle Crossland ,  Martha Wright ,  Ellis Merlin ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James Linder ,  Robert Miller ,  Bruce Skillings ,  Alan Mousel ,  Albert Hornbrook ,  Christopher Clucas ,  Moez Meghji ,  Andreas Tanner ,  Francis Cassagne ,  Gilles Pollini ,  Terry Colbert ,  Francis Cammack

发明人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Vance Kramer ,  Gregory Warren ,  Steve Evola ,  Lyle Crossland ,  Martha Wright ,  Ellis Merlin ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James Linder ,  Robert Miller ,  Bruce Skillings ,  Alan Mousel ,  Albert Hornbrook ,  Christopher Clucas ,  Moez Meghji ,  Andreas Tanner ,  Francis Cassagne ,  Gilles Pollini ,  Terry Colbert ,  Francis Cammack

IPC分类号： A01H1/00 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04

CPC分类号： C12N9/12 ,  C07K14/325 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.

公开(公告)号：US20060021095A1

公开(公告)日：2006-01-26

申请号：US10755092

申请日：2004-01-08

申请人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola ,  Martha Wright ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi

发明人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola ,  Martha Wright ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N15/82

CPC分类号： C07K14/325 ,  C12N9/12 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

公开(公告)号：US06403865B1

公开(公告)日：2002-06-11

申请号：US08438666

申请日：1995-05-10

申请人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James O. Linder ,  Robert L. Miller ,  Bruce W. Skillings ,  Alan W. Mousel ,  Albert R. Hornbrook ,  Christopher P. Clucas ,  Moez Rajabali Meghji ,  Andreas H. Tanner ,  Francis E. Cassagne ,  Gilles Pollini ,  Terry Ray Colbert ,  Francis P. Cammack

发明人： Michael G. Koziel ,  Nalini M. Desai ,  Kelly S. Lewis ,  Vance C. Kramer ,  Gregory W. Warren ,  Stephen V. Evola ,  Lyle D. Crossland ,  Martha S. Wright ,  Ellis J. Merlin ,  Karen L. Launis ,  Steven J. Rothstein ,  Cindy G. Bowman ,  John L. Dawson ,  Erik M. Dunder ,  Gary M. Pace ,  Janet L. Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James O. Linder ,  Robert L. Miller ,  Bruce W. Skillings ,  Alan W. Mousel ,  Albert R. Hornbrook ,  Christopher P. Clucas ,  Moez Rajabali Meghji ,  Andreas H. Tanner ,  Francis E. Cassagne ,  Gilles Pollini ,  Terry Ray Colbert ,  Francis P. Cammack

IPC分类号： A01H500

CPC分类号： C12N9/12 ,  C07K14/325 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.

摘要翻译： 公开了用感兴趣的核酸序列转化玉米的方法。 该方法包括使未成熟的合子胚或I型愈伤组织进行高速微粒轰击。 该方法能够生产具有商业重要性的转化玉米品系及其杂交组合。

公开(公告)号：US20080070829A1

公开(公告)日：2008-03-20

申请号：US11763947

申请日：2007-06-15

申请人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： A01N37/18 ,  A01H1/00 ,  A01H5/00 ,  A01P5/00 ,  C12N1/00 ,  C12N15/63 ,  C12P21/00 ,  C12N5/04 ,  C12N1/21 ,  C07H21/00 ,  A01P15/00 ,  A01H5/10

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-50047，B-30959，B-30960，B-30943，B-50048或B-50048，以及其变体和片段。

公开(公告)号：US08147856B2

公开(公告)日：2012-04-03

申请号：US12364335

申请日：2009-02-02

申请人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai ,  Shruti Agarwal ,  Daniel John Tomso ,  Rong Guo ,  Julia Daum

发明人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai ,  Shruti Agarwal ,  Daniel John Tomso ,  Rong Guo ,  Julia Daum

IPC分类号： A01N63/02 ,  C12N15/32 ,  C12P21/04 ,  C07K14/325

CPC分类号： C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

公开(公告)号：US08541366B2

公开(公告)日：2013-09-24

申请号：US12209354

申请日：2008-09-12

申请人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso ,  Vadim Beilinson ,  Theodore Kahn

发明人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso ,  Vadim Beilinson ,  Theodore Kahn

IPC分类号： A01N37/18 ,  C07K14/325

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided, particularly synthetically-derived coding sequences. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, or 18, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 6, 7, 8, 10, 12, 14, 16, or 17, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供包含δ-内毒素多肽的编码序列的组合物，特别是合成来源的编码序列。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：9,11,13,15或18所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2，4，6，7，8，10，12，14，16或17，以及其变体和片段。

公开(公告)号：US07923602B2

公开(公告)日：2011-04-12

申请号：US11762886

申请日：2007-06-14

申请人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai

发明人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32 ,  C12N1/21

CPC分类号： C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

公开(公告)号：US20090181116A1

公开(公告)日：2009-07-16

申请号：US12364335

申请日：2009-02-02

申请人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai ,  Shruti Agarwal ,  Daniel John Tomso ,  Rong Guo ,  Julia Daum

发明人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai ,  Shruti Agarwal ,  Daniel John Tomso ,  Rong Guo ,  Julia Daum

IPC分类号： A01N63/02 ,  C07K14/325 ,  C07K16/12 ,  C12P21/02 ,  A01N33/08 ,  A01P7/04

CPC分类号： C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

公开(公告)号：US20090126044A1

公开(公告)日：2009-05-14

申请号：US12249016

申请日：2008-10-10

申请人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso

发明人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/64 ,  C12N1/21 ,  C12N5/14 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions containing a synthetic nucleotide sequence encoding a Cry1Ac protein are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided, wherein the nucleotide sequences are set forth in SEQ ID NO:1, 2, 3, 4, 5 or 6, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了含有编码Cry1Ac蛋白质的合成核苷酸序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子，其中核苷酸序列如SEQ ID NO：1,2,3,4,5或6所示，以及其变体和片段。