公开(公告)号：US20060117407A1

公开(公告)日：2006-06-01

申请号：US11071896

申请日：2005-03-02

申请人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Vance Kramer ,  Gregory Warren ,  Steve Evola ,  Lyle Crossland ,  Martha Wright ,  Ellis Merlin ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James Linder ,  Robert Miller ,  Bruce Skillings ,  Alan Mousel ,  Albert Hornbrook ,  Christopher Clucas ,  Moez Meghji ,  Andreas Tanner ,  Francis Cassagne ,  Gilles Pollini ,  Terry Colbert ,  Francis Cammack

发明人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Vance Kramer ,  Gregory Warren ,  Steve Evola ,  Lyle Crossland ,  Martha Wright ,  Ellis Merlin ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi ,  Annick De Framond ,  James Linder ,  Robert Miller ,  Bruce Skillings ,  Alan Mousel ,  Albert Hornbrook ,  Christopher Clucas ,  Moez Meghji ,  Andreas Tanner ,  Francis Cassagne ,  Gilles Pollini ,  Terry Colbert ,  Francis Cammack

IPC分类号： A01H1/00 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04

CPC分类号： C12N9/12 ,  C07K14/325 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.

公开(公告)号：US20060021095A1

公开(公告)日：2006-01-26

申请号：US10755092

申请日：2004-01-08

申请人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola ,  Martha Wright ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi

发明人： Michael Koziel ,  Nalini Desai ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola ,  Martha Wright ,  Karen Launis ,  Steven Rothstein ,  Cindy Boyce ,  John Dawson ,  Erik Dunder ,  Gary Pace ,  Jan Suttie ,  Nadine Carozzi

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N15/82

CPC分类号： C07K14/325 ,  C12N9/12 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

公开(公告)号：US06720488B2

公开(公告)日：2004-04-13

申请号：US09988462

申请日：2001-11-20

申请人： Michael Koziel ,  Nalini Desai ,  Gary M. Pace ,  Jan Suttie ,  Nadine Carozzi ,  Cindy Boyce ,  John L. Dawson ,  Erik Dunder ,  Martha Wright ,  Karen Launis ,  Steven J. Rothstein ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola

发明人： Michael Koziel ,  Nalini Desai ,  Gary M. Pace ,  Jan Suttie ,  Nadine Carozzi ,  Cindy Boyce ,  John L. Dawson ,  Erik Dunder ,  Martha Wright ,  Karen Launis ,  Steven J. Rothstein ,  Kelly Lewis ,  Gregory Warren ,  Steve Evola

IPC分类号： A01H510

CPC分类号： C07K14/325 ,  C12N9/12 ,  C12N9/88 ,  C12N15/8286 ,  Y02A40/162

摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

摘要翻译： 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子，特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。

公开(公告)号：US20080070829A1

公开(公告)日：2008-03-20

申请号：US11763947

申请日：2007-06-15

申请人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： A01N37/18 ,  A01H1/00 ,  A01H5/00 ,  A01P5/00 ,  C12N1/00 ,  C12N15/63 ,  C12P21/00 ,  C12N5/04 ,  C12N1/21 ,  C07H21/00 ,  A01P15/00 ,  A01H5/10

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-50047，B-30959，B-30960，B-30943，B-50048或B-50048，以及其变体和片段。

公开(公告)号：US20070240239A1

公开(公告)日：2007-10-11

申请号：US11765491

申请日：2007-06-20

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： C12N15/63 ,  A01H1/00 ,  A01H5/00 ,  A01H7/00 ,  C12N5/04 ,  C12N5/14 ,  C12N15/82 ,  C07H21/04 ,  C12N1/20

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体 。

公开(公告)号：US20070218530A1

公开(公告)日：2007-09-20

申请号：US11748727

申请日：2007-05-15

申请人： Nicholas Duck ,  Brian Carr ,  Michael Koziel ,  Nadine Carozzi ,  Brian Vande Berg

发明人： Nicholas Duck ,  Brian Carr ,  Michael Koziel ,  Nadine Carozzi ,  Brian Vande Berg

IPC分类号： C12P19/04 ,  C12P19/02 ,  C12P19/12

CPC分类号： C12P19/14

摘要： Compositions and methods for biomass conversion are provided. Compositions comprise novel enzyme mixtures that can be used directly on lignocellulose substrate. Methods involve converting lignocellulosic biomass to free sugars and small oligosaccharides with enzymes that break down lignocellulose. Novel combinations of enzymes are provided that provide a synergistic release of sugars from plant biomass. Also provided are methods to identify enzymes, strains producing enzymes, or genes that encode enzymes capable of degrading lignocellulosic material to generate sugars.

摘要翻译： 提供了生物质转化的组合物和方法。 组合物包含可直接用于木质纤维素底物的新型酶混合物。 方法涉及将木质纤维素生物质转化为游离糖和小分子糖，以分解木质纤维素酶。 提供了新的酶组合，其提供来自植物生物质的糖的协同释放。 还提供了鉴定酶，产生酶的菌株或编码能够降解木质纤维素材料以产生糖的酶的基因的方法。

公开(公告)号：US20050183161A1

公开(公告)日：2005-08-18

申请号：US10958008

申请日：2004-10-04

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： C07K14/325 ,  C12N15/82 ,  A01H1/00 ,  A01N63/00 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子及其变体。

公开(公告)号：US20050049410A1

公开(公告)日：2005-03-03

申请号：US10926819

申请日：2004-08-26

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： A01N63/02 ,  C07H21/04 ,  C07K14/325 ,  C12N15/82 ,  C12N1/20

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体 。

公开(公告)号：US20060242732A1

公开(公告)日：2006-10-26

申请号：US11396808

申请日：2006-04-03

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Theodore Kahn

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Theodore Kahn

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04 ,  C12N1/21 ,  C07K14/325

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS:1, 10 and 12, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,11和13所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,10和12所示的核苷酸序列，作为 以及其变体和片段。

公开(公告)号：US20060212965A1

公开(公告)日：2006-09-21

申请号：US11343533

申请日：2006-01-31

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck

IPC分类号： A01H1/00 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04 ,  A01N63/00 ,  C07K14/415

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8281 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4或6所示氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列 ，以及其变体和片段。