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    Large comb type branched polynucleotides
    8.
    发明授权
    Large comb type branched polynucleotides 失效
    大梳型支链多核苷酸

    公开(公告)号：US5710264A

    公开(公告)日：1998-01-20

    申请号：US478085

    申请日：1995-06-07

    申请人： Michael S. Urdea ,  Thomas Horn ,  Chu-An Chang ,  Brian Warner ,  Timothy J. Fultz

    发明人： Michael S. Urdea ,  Thomas Horn ,  Chu-An Chang ,  Brian Warner ,  Timothy J. Fultz

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/682

    摘要： Large comb-type branched polynucleotides useful as amplifier molecules in nucleic acid hybridization assays are provided, the polynucleotides comprising: a polynucleotide backbone having at least about 15 multifunctional nucleotides, each of which defines a sidechain site and a first single-stranded oligonucleotide unit that is capable of binding specifically to a first single-stranded polynucleotide sequence of interest; and pendant polynucleotide sidechains extending from said multifunctional nucleotides each comprising iterations of a second single-stranded oligonucleotide unit that is capable of binding specifically to a second single-stranded polynucleotide sequence of interest, the total number of iterations in all sidechains being at least 20. Methods of making these polynucleotides are also provided.

    摘要翻译： 提供了用作核酸杂交测定中的放大分子的大梳状支化多核苷酸，所述多核苷酸包含：多核苷酸主链，其具有至少约15个多功能核苷酸，每个核苷酸限定侧链位点和第一单链寡核苷酸单元， 能够特异性结合目标的第一单链多核苷酸序列; 和从所述多功能核苷酸延伸的侧挂多核苷酸侧链，其每个包括能够特异性结合目标的第二单链多核苷酸序列的第二单链寡核苷酸单元的迭代，所有侧链的总迭代次数至少为20。 还提供制备这些多核苷酸的方法。

    HTLV-1 probes for use in solution phase sandwich hybridization assays
    10.
    发明授权
    HTLV-1 probes for use in solution phase sandwich hybridization assays 失效
    用于溶液相夹心杂交测定的HTLV-1探针

    公开(公告)号：US06235465B1

    公开(公告)日：2001-05-22

    申请号：US08427569

    申请日：1995-04-24

    申请人： Janice A. Kolberg ,  Michael S. Urdea

    发明人： Janice A. Kolberg ,  Michael S. Urdea

    IPC分类号： C12Q170

    CPC分类号： G11B5/4833 ,  C12Q1/682 ,  C12Q1/702

    摘要： Synthetic oligonucleotides which are useful as probes in a solution phase sandwich hybridization assay for the detection of HTLV-1 are described. The oligonucleotides have a first segment comprising a nucleotide sequence substantially complementary to a segment of HTLV-1 nucleic acid, and a second segment comprising a nucleotide sequence which is not complementary to HTLV-1 nucleic acid but is substantially complementary to an oligonucleotide unit of a nucleic acid multimer or to an oligonucleotide bound to a solid phase. Amplified nucleic acid hybridization assays using the probes are exemplified.

    摘要翻译： 描述了用于检测HTLV-1的溶液相夹心杂交测定中用作探针的合成寡核苷酸。 寡核苷酸具有包含与HTLV-1核酸片段基本上互补的核苷酸序列的第一片段，以及第二片段，其包含与HTLV-1核酸不互补的核苷酸序列，但与 核酸多聚体或与固相结合的寡核苷酸。 使用探针进行扩增核酸杂交测定。