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    Apparatus for high-throughput cell culture with mechanical compression stimulation
    1.
    发明授权
    Apparatus for high-throughput cell culture with mechanical compression stimulation 有权
    用于具有机械压缩刺激的高通量细胞培养的装置

    公开(公告)号：US08980624B2

    公开(公告)日：2015-03-17

    申请号：US12651905

    申请日：2010-01-04

    申请人： Min-Hsien Wu ,  Chun-Li Lin ,  Shiao-Wen Tsai ,  Heng-Liang Liu ,  Yen-Ting Liu

    发明人： Min-Hsien Wu ,  Chun-Li Lin ,  Shiao-Wen Tsai ,  Heng-Liang Liu ,  Yen-Ting Liu

    IPC分类号： C12M1/00 ,  C12M3/00 ,  C12M3/06 ,  C12M1/12 ,  C12M1/42

    CPC分类号： C12M23/44 ,  C12M29/10 ,  C12M29/14 ,  C12M35/04

    摘要： An apparatus for high-throughput cell culture with mechanical compression stimulation includes a cell culture vessel and a fluid pressure supply unit. The cell culture vessel includes at least one culture chamber, at least one pressure chamber disposed above the culture chamber, a membrane disposed between the culture chamber and the pressure chamber, and at least one pressurizing member that projects downwardly from the membrane into the culture chamber. The fluid pressure supply unit is connected fluidly to the pressure chamber, and has a fluid pressure supply device to supply a pressurized fluid to the pressure chamber so as to deform the membrane and move the pressurizing member, and a control device that is adapted to vary a pressure of the pressurized fluid in the pressure chamber.

    摘要翻译： 用于具有机械压缩刺激的高通量细胞培养的装置包括细胞培养容器和流体压力供应单元。 细胞培养容器包括至少一个培养室，设置在培养室上方的至少一个压力室，设置在培养室和压力室之间的膜，以及至少一个从膜向下突出到培养室中的加压构件 。 流体压力供应单元流体地连接到压力室，并且具有流体压力供应装置，以将加压流体供应到压力室，以使膜变形并移动加压构件，以及适于变化的控制装置 压力室中加压流体的压力。

    APPARATUS FOR HIGH-THROUGHPUT CELL CULTURE WITH MECHANICAL COMPRESSION STIMULATION
    2.
    发明申请
    APPARATUS FOR HIGH-THROUGHPUT CELL CULTURE WITH MECHANICAL COMPRESSION STIMULATION 有权
    用于具有机械压缩刺激的高通量细胞培养物的装置

    公开(公告)号：US20110076758A1

    公开(公告)日：2011-03-31

    申请号：US12651905

    申请日：2010-01-04

    申请人： Min-Hsien Wu ,  Chun-Li Lin ,  Shiao-Wen Tsai ,  Heng-Liang Liu ,  Yen-Ting Liu

    发明人： Min-Hsien Wu ,  Chun-Li Lin ,  Shiao-Wen Tsai ,  Heng-Liang Liu ,  Yen-Ting Liu

    IPC分类号： C12M1/12

    CPC分类号： C12M23/44 ,  C12M29/10 ,  C12M29/14 ,  C12M35/04

    摘要： An apparatus for high-throughput cell culture with mechanical compression stimulation includes a cell culture vessel and a fluid pressure supply unit. The cell culture vessel includes at least one culture chamber, at least one pressure chamber disposed above the culture chamber, a membrane disposed between the culture chamber and the pressure chamber, and at least one pressurizing member that projects downwardly from the membrane into the culture chamber. The fluid pressure supply unit is connected fluidly to the pressure chamber, and has a fluid pressure supply device to supply a pressurized fluid to the pressure chamber so as to deform the membrane and move the pressurizing member, and a control device that is adapted to vary a pressure of the pressurized fluid in the pressure chamber.

    摘要翻译： 用于具有机械压缩刺激的高通量细胞培养的装置包括细胞培养容器和流体压力供应单元。 细胞培养容器包括至少一个培养室，设置在培养室上方的至少一个压力室，设置在培养室和压力室之间的膜，以及至少一个从膜向下突出到培养室中的加压构件 。 流体压力供应单元流体地连接到压力室，并且具有流体压力供应装置，以将加压流体供应到压力室，以使膜变形并移动加压构件，以及适于变化的控制装置 压力室中加压流体的压力。

    Detection Method for Sensor Membrane of EuTixOy as Part of a Biosensor by Using PNIPAAm for Wrapping Enzymes
    3.
    发明申请
    Detection Method for Sensor Membrane of EuTixOy as Part of a Biosensor by Using PNIPAAm for Wrapping Enzymes 有权
    EuTixOy传感器膜的检测方法作为生物传感器的一部分，通过使用PNIPAAm包裹酶

    公开(公告)号：US20130032492A1

    公开(公告)日：2013-02-07

    申请号：US13478161

    申请日：2012-05-23

    申请人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

    发明人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

    IPC分类号： G01N27/414

    CPC分类号： C12Q1/001 ,  G01N27/227

    摘要： A detection method for a sensor membrane formed of EuTixOy as part of a biosensor by using PNIPAAm for wrapping enzymes is provided with adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1× PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. wherein the biosensor is an EIS sensor having a sensor membrane formed of EuTixOy; and taking a measurement.

    摘要翻译： 提供了通过使用PNIPAAm包裹酶作为生物传感器的一部分的EuTixOy形成的传感器膜的检测方法，在20ml水中加入1.0g NIPAAm粉末，在60℃下加热，形成NIPAAm溶液，并冷却 NIPAAm解决方案 向NIPAAm溶液中加入200μl98.7wt％的APS和50μl99wt％的TEMED，均匀混合，并使混合物反应30小时以制备透明的凝胶PNIPAAm; 向100μl1×PBS缓冲溶液中加入5mg酶，均匀混合，向缓冲液中加入100μlPNIPAAm，均匀混合缓冲溶液; 将生物传感器放置在加热器上，在37℃的恒温下加热，其中生物传感器是具有由EuTixOy形成的传感器膜的EIS传感器; 并进行测量。

    Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes
    4.
    发明授权
    Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes 有权
    铕氧化钛传感器膜的检测方法，作为使用PNIPAAm包裹酶的生物传感器的一部分

    公开(公告)号：US08951403B2

    公开(公告)日：2015-02-10

    申请号：US13478161

    申请日：2012-05-23

    申请人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

    发明人： Tung-Ming Pan ,  Chao-Wen Lin ,  Kung-Yuan Chang ,  Min-Hsien Wu ,  Shiao-Wen Tsai

    IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/22

    CPC分类号： C12Q1/001 ,  G01N27/227

    摘要： A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1×PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTixOy; and taking a measurement.

    摘要翻译： 由使用PNIPAAm包裹酶作为生物传感器的一部分的由氧化铕形成的传感器膜的检测方法包括将1.0g NIPAAm粉末加入到20ml水中，在60℃下加热，形成NIPAAm溶液，并冷却 NIPAAm解决方案 向NIPAAm溶液中加入200μl98.7wt％的APS和50μl99wt％的TEMED，均匀混合，并使混合物反应30小时以制备透明的凝胶PNIPAAm; 向100μl1×PBS缓冲溶液中加入5mg酶，均匀混合，向缓冲液中加入100μlPNIPAAm，均匀混合缓冲溶液; 将生物传感器放置在加热器上，在37℃的恒温下加热，生物传感器是具有由EuTixOy形成的传感器膜的EIS传感器; 并进行测量。

    Method for producing cross-linked hyaluronic acid-protein bio-composites
    7.
    发明申请
    Method for producing cross-linked hyaluronic acid-protein bio-composites 审中-公开
    交联透明质酸 - 蛋白质生物复合材料的制备方法

    公开(公告)号：US20060189516A1

    公开(公告)日：2006-08-24

    申请号：US11208596

    申请日：2005-08-23

    申请人： Chiung-Lin Yang ,  Jui-Hsiang Chen ,  Shiao-Wen Tsai ,  Hsin-Nung Shih ,  Lih-Yuann Shih

    发明人： Chiung-Lin Yang ,  Jui-Hsiang Chen ,  Shiao-Wen Tsai ,  Hsin-Nung Shih ,  Lih-Yuann Shih

    IPC分类号： A61K38/16 ,  C07K14/47

    CPC分类号： A61L27/58 ,  A61L27/20 ,  C08L5/08

    摘要： This invention is concerned with a new method for producing cross-linked hyaluronic acid—protein bio-composites in various shapes. In the present process, a polysaccharide solution and a protein solution are mixed under moderate pH values in presence of salts to form a homogenous solution, which can be processed into various shapes, such as membrane, sponge, fiber, tube or micro-granular and so on. After then, the homogenous solution is subjected to a cross-linking reaction in organic solvent containing weak acid to produce an implantable bio composite material having excellent bio-compatibility, biodegradability, prolonged enzymatic degradation time, and good physical properties.

    摘要翻译： 本发明涉及用于生产各种形状的交联透明质酸 - 蛋白质生物复合材料的新方法。 在本方法中，多糖溶液和蛋白质溶液在盐的存在下在中等pH值下混合以形成均匀的溶液，其可以加工成各种形状，例如膜，海绵，纤维，管或微粒，以及 所以。 然后，均匀溶液在含有弱酸的有机溶剂中进行交联反应，生成具有优异的生物相容性，生物降解性，延长的酶降解时间和良好物理性质的可植入生物复合材料。

    Method of preparing microsphere composite of collagen and bioceramic powder
    8.
    发明授权
    Method of preparing microsphere composite of collagen and bioceramic powder 失效
    胶原和生物陶瓷粉末微球复合材料的制备方法

    公开(公告)号：US06752938B2

    公开(公告)日：2004-06-22

    申请号：US09977591

    申请日：2001-10-13

    申请人： Yng-Jiin Wang ,  Shiao-Wen Tsai ,  Hsiu-Hsuan Huang ,  Wen Chung Chang

    发明人： Yng-Jiin Wang ,  Shiao-Wen Tsai ,  Hsiu-Hsuan Huang ,  Wen Chung Chang

    IPC分类号： B29B900

    CPC分类号： A61L27/48 ,  A61L27/34 ,  A61L27/46 ,  C08L5/08 ,  C08L5/04 ,  C08L89/06

    摘要： This invention provides a method of preparing microsphere composite of collagen and bioceramic powder. This method first mixes a collagen solution with bioceramic powder and alginate, and squeezes the mixture solution to spherical droplets being discharged into a divalent cation solution undergoing gelling to form microspheres. Next, the microsphere is coated with a chitosan solution. Then, interior alginate and surface chitosan of the microsphere are liquefied and washed out with an aqueous buffer solution such as phosphate based buffer, and collagen in the microsphere is reconstituted to fiber network at the same time. The prepared microsphere composite has similar composition components of bone tissue, and the collagen thereof has a network of reconstituted fibers. The microsphere composite of this invention provides a similar growth environment of bone tissue cells, is used as a carrier to carry cells, coat and fix bone growth factors, and is applied in bone repair.

    摘要翻译： 本发明提供一种制备胶原和生物陶瓷粉末的微球复合材料的方法。 该方法首先将胶原溶液与生物陶瓷粉末和藻酸盐混合，并将混合物溶液挤压成球形小滴，将其排出成经历凝胶化的二价阳离子溶液以形成微球体。 接着，将微球涂覆有壳聚糖溶液。 然后，将微球的内部藻酸盐和表面壳聚糖液化并用缓冲液等缓冲溶液洗涤，同时将微球中的胶原重构成纤维网。 制备的微球复合材料具有相似的骨组织组成成分，其胶原蛋白具有复原纤维网。 本发明的微球复合材料提供了类似的骨组织细胞生长环境，用作携带细胞，涂覆和固定骨生长因子的载体，并用于骨修复。