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    METHODS FOR HPLC ANALYSIS
    1.
    发明申请
    METHODS FOR HPLC ANALYSIS 有权

    公开(公告)号：US20210163919A1

    公开(公告)日：2021-06-03

    申请号：US16639305

    申请日：2018-08-17

    Applicant: ModernaTX, Inc.

    Inventor： William Issa ,  Meredith Packer

    IPC: C12N15/10 ,  G01N30/02

    Abstract: Aspects of the disclosure relate to liquid chromatography (e.g., HPLC) methods which enable high resolution separations of polynucleotides having hydrophobic portions (e.g., polyadenylated nucleic acids, such as mRNA) based upon the hydrophobic character of the molecules (e.g., polyA tail length). In some embodiments, the disclosure describes liquid chromatographic methods for separating a nucleic acid having a hydrophobic portion (e.g., a polyadenylated nucleic acid, such as an mRNA) from a complex mixture by a mobile phase system that comprises an ion pairing agent selected from Tris, inorganic cations (including e.g., Na, Li, K, ammonium, etc.), biological buffers (e.g., MOPS, HEPES, PIPES, etc.), and other charged or hydrophilic moieties, and lacks conventional ion pairing agents (e.g., Triethylammonium acetate, TEAA). Accordingly, in some embodiments methods described by the disclosure are useful for assessing the quality of pharmaceutical preparations comprising nucleic acids.

    ANALYTICAL HPLC METHODS
    4.
    发明申请
    ANALYTICAL HPLC METHODS 审中-公开

    公开(公告)号：US20200239869A1

    公开(公告)日：2020-07-30

    申请号：US16639265

    申请日：2018-08-17

    Applicant: ModernaTX, Inc.

    Inventor： William Issa ,  Meredith Packer

    IPC: C12N15/10 ,  C12Q1/6876 ,  A61K48/00 ,  G01N30/34

    Abstract: Aspects of the disclosure relate to liquid chromatography (e.g., HPLC) methods which enable high resolution separations of polynucleotides of various lengths, sequences, and/or base compositions in a highly tunable manner. In some embodiments, the disclosure describes liquid chromatographic methods for separating a nucleic acid (e.g., a polyadenylated nucleic acid, such as an mRNA) from a complex mixture by using multiple ion pairing agents in the same mobile phase system. Accordingly, in some embodiments methods described by the disclosure are useful for assessing the quality of pharmaceutical preparations comprising nucleic acids.

    Analytical HPLC methods
    6.
    发明授权
    Analytical HPLC methods 有权

    公开(公告)号：US11866696B2

    公开(公告)日：2024-01-09

    申请号：US16639265

    申请日：2018-08-17

    Applicant: ModernaTX, Inc.

    Inventor： William Issa ,  Meredith Packer

    IPC: C12N15/10 ,  A61K48/00 ,  C12Q1/6876 ,  G01N30/34 ,  G01N30/02 ,  G01N30/88

    CPC classification number: C12N15/101 ,  A61K48/0091 ,  C12Q1/6876 ,  G01N30/34 ,  G01N2030/027 ,  G01N2030/8827

    Abstract: Aspects of the disclosure relate to liquid chromatography (e.g., HPLC) methods which enable high resolution separations of polynucleotides of various lengths, sequences, and/or base compositions in a highly tunable manner. In some embodiments, the disclosure describes liquid chromatographic methods for separating a nucleic acid (e.g., a polyadenylated nucleic acid, such as an mRNA) from a complex mixture by using multiple ion pairing agents in the same mobile phase system. Accordingly, in some embodiments methods described by the disclosure are useful for assessing the quality of pharmaceutical preparations comprising nucleic acids.

    EXTRACTION-LESS REVERSE PHASE (RP) CHROMATOGRAPHY OF MRNA ENCAPSULATED IN LIPID NANOPARTICLES FOR MRNA PURITY ASSESSMENT
    8.
    发明申请
    EXTRACTION-LESS REVERSE PHASE (RP) CHROMATOGRAPHY OF MRNA ENCAPSULATED IN LIPID NANOPARTICLES FOR MRNA PURITY ASSESSMENT 有权

    公开(公告)号：US20250092440A1

    公开(公告)日：2025-03-20

    申请号：US18294279

    申请日：2022-08-01

    Applicant: ModernaTX, Inc.

    Inventor： Meredith Packer ,  Kevin Cognata

    IPC: C12Q1/6811 ,  B01D15/32

    Abstract: Aspects of the disclosure relate to liquid chromatography (e.g., HPLC) methods which enable identification of one or more target nucleic acids in a mixture (e.g., pharmaceutical composition). The disclosure is based, in part, on methods that allow for addition of pharmaceutical compositions (e.g., lipid-based pharmaceutical compositions) directly onto a chromatographic column without the need for first separating target nucleic acids out of the composition. Accordingly, in some embodiments, methods described by the disclosure are useful for assessing the quality of pharmaceutical preparations comprising nucleic acids.

    Methods for HPLC analysis
    9.
    发明授权
    Methods for HPLC analysis 有权

    公开(公告)号：US11912982B2

    公开(公告)日：2024-02-27

    申请号：US16639305

    申请日：2018-08-17

    Applicant: ModernaTX, Inc.

    Inventor： William Issa ,  Meredith Packer

    IPC: C12N15/10 ,  G01N30/02 ,  G01N30/88

    CPC classification number: C12N15/101 ,  G01N30/02 ,  G01N2030/8827 ,  G01N2496/00

    Abstract: Aspects of the disclosure relate to liquid chromatography (e.g., HPLC) methods which enable high resolution separations of polynucleotides having hydrophobic portions (e.g., polyadenylated nucleic acids, such as mRNA) based upon the hydrophobic character of the molecules (e.g., polyA tail length). In some embodiments, the disclosure describes liquid chromatographic methods for separating a nucleic acid having a hydrophobic portion (e.g., a polyadenylated nucleic acid, such as an mRNA) from a complex mixture by a mobile phase system that comprises an ion pairing agent selected from Tris, inorganic cations (including e.g., Na, Li, K, ammonium, etc.), biological buffers (e.g., MOPS, HEPES, PIPES, etc.), and other charged or hydrophilic moieties, and lacks conventional ion pairing agents (e.g., Triethylammonium acetate, TEAA). Accordingly, in some embodiments methods described by the disclosure are useful for assessing the quality of pharmaceutical preparations comprising nucleic acids.

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