公开(公告)号：US12023371B2

公开(公告)日：2024-07-02

申请号：US17127435

申请日：2020-12-18

申请人： ModernaTX, Inc.

发明人： Tirtha Chakraborty ,  Stephane Bancel ,  Stephen G. Hoge ,  Atanu Roy ,  Antonin De Fougerolles ,  Noubar B. Afeyan

IPC分类号： A61K39/00 ,  C12N15/67

CPC分类号： A61K39/00 ,  C12N15/67

摘要： The invention relates to compositions and methods for the manufacture and optimization of modified mRNA molecules via optimization of their terminal architecture.

公开(公告)号：US10155029B2

公开(公告)日：2018-12-18

申请号：US15429532

申请日：2017-02-10

申请人： ModernaTX, Inc.

发明人： Tirtha Chakraborty ,  Stephane Bancel ,  Stephen G. Hoge ,  Atanu Roy ,  Antonin De Fougerolles ,  Noubar B. Afeyan

IPC分类号： A61K39/00 ,  C12N15/67

摘要： The invention relates to compositions and methods for the manufacture and optimization of modified mRNA molecules via optimization of their terminal architecture.

公开(公告)号：US10590161B2

公开(公告)日：2020-03-17

申请号：US14777320

申请日：2014-03-14

申请人： ModernaTX, Inc.

发明人： William Joseph Issa ,  Joseph Louis Barberio ,  John Grant Aunins ,  Noubar B. Afeyan

IPC分类号： C07H21/02 ,  C12N15/10 ,  G01N30/96 ,  B01D15/36 ,  G01N30/88

摘要： The current landscape for preparative chromatographic RNA purification uses reversed phase HPLC, but this technique presents many issues with process scale up and ion exchange for preparative purification has only been used for short RNAs. The invention provides preparative purification of RNA (e.g., mRNA) using ion (e.g., anion) exchange chromatography that allows for separation of longer RNAs up to 10,000 nucleotides in length via a scalable method. This method avoids problems with current techniques by using low pressure chromatography that is agreeable with existing equipment in cGMP commercial facilities, that uses aqueous-bases solutions as the mobile phase (rather than flammable of greater than 10 mg RNA/mL resin (e.g., using larger pore sorbents, >500 Angstroms, that display greater mRNA binding capacities), and that yields desired RNA salt forms for downstream formulation with no additional manipulation necessary (unlike ion pair reverse phase techniques).

公开(公告)号：US09937233B2

公开(公告)日：2018-04-10

申请号：US15248053

申请日：2016-08-26

申请人： ModernaTX, Inc.

发明人： Jason P. Schrum ,  Stephane Bancel ,  Noubar B. Afeyan ,  Kenechi Ejebe

IPC分类号： A61K31/70 ,  C07H21/04 ,  A61K38/19 ,  A61K48/00 ,  A61K31/495 ,  A61K39/395 ,  A01K67/00

CPC分类号： A61K38/193 ,  A61K31/395 ,  A61K31/495 ,  A61K31/7088 ,  A61K31/7115 ,  A61K38/00 ,  A61K38/13 ,  A61K38/195 ,  A61K38/28 ,  A61K39/3955 ,  A61K48/00 ,  A61K48/0066 ,  A61K48/0075 ,  A61K48/0083 ,  C07K14/62 ,  C07K14/685 ,  A61K2300/00

摘要： Provided are compositions and methods for delivering biological moieties such as modified nucleic acids into cells to modulate protein expression. Such compositions and methods include the use of modified messenger RNAs, and are useful to treat or prevent diseases, disorders or conditions, or to improve a subject's heath or wellbeing.

公开(公告)号：US20240123035A1

公开(公告)日：2024-04-18

申请号：US18177455

申请日：2023-03-02

申请人： ModernaTX, Inc.

发明人： Antonin DE FOUGEROLLES ,  Kristy M. Wood ,  Sayda M. ELBASHIR ,  Noubar B. Afeyan ,  Pedro VALENCIA ,  Jason P. SCHRUM

IPC分类号： A61K38/19 ,  A61K9/00 ,  A61K9/127 ,  A61K9/14 ,  A61K9/16 ,  A61K31/7088 ,  A61K31/7105 ,  A61K38/48 ,  A61K48/00 ,  C07K2/00 ,  C07K14/535 ,  C12N15/00 ,  C12N15/117 ,  C12N15/88 ,  C12P21/00

CPC分类号： A61K38/193 ,  A61K9/0019 ,  A61K9/0024 ,  A61K9/0048 ,  A61K9/1271 ,  A61K9/1272 ,  A61K9/14 ,  A61K9/16 ,  A61K9/1647 ,  A61K31/7088 ,  A61K31/7105 ,  A61K38/4833 ,  A61K48/00 ,  A61K48/0033 ,  A61K48/0041 ,  A61K48/005 ,  A61K48/0066 ,  C07K2/00 ,  C07K14/535 ,  C12N15/00 ,  C12N15/117 ,  C12N15/88 ,  C12P21/00

摘要： The present disclosure provides, inter alia, formulation compositions comprising modified nucleic acid molecules which may encode a protein, a protein precursor, or a partially or fully processed form of the protein or a protein precursor. The formulation composition may further include a modified nucleic acid molecule and a delivery agent. The present invention further provides nucleic acids useful for encoding polypeptides capable of modulating a cell's function and/or activity.

公开(公告)号：US09925277B2

公开(公告)日：2018-03-27

申请号：US15021287

申请日：2014-09-12

申请人： ModernaTX, Inc.

发明人： Örn Almarsson ,  Noubar B. Afeyan ,  Joseph Beene Bolen ,  Staci Sabnis

IPC分类号： A61K48/00 ,  A61K31/198 ,  A61K38/00 ,  A61K9/00 ,  A61K31/401 ,  A61K31/405 ,  A61K31/4172

CPC分类号： A61K48/0066 ,  A61K9/0019 ,  A61K31/198 ,  A61K31/401 ,  A61K31/405 ,  A61K31/4172 ,  A61K38/00

摘要： The present invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides formulations containing amino acids.

公开(公告)号：US09597380B2

公开(公告)日：2017-03-21

申请号：US14043927

申请日：2013-10-02

申请人： ModernaTX, Inc.

发明人： Tirtha Chakraborty ,  Stephane Bancel ,  Stephen G. Hoge ,  Atanu Roy ,  Antonin de Fougerolles ,  Noubar B. Afeyan

IPC分类号： A61K39/00 ,  A61K48/00 ,  C07H21/04 ,  C12N15/67

CPC分类号： A61K39/00 ,  C12N15/67

摘要： The invention relates to compositions and methods for the manufacture and optimization of modified mRNA molecules via optimization of their terminal architecture.