公开(公告)号：US20030032052A1

公开(公告)日：2003-02-13

申请号：US10262476

申请日：2002-09-30

申请人： Molecular Dynamics, Inc.

发明人： Andy Hadd ,  Stevan Jovanovich

IPC分类号： C12Q001/68

CPC分类号： C12Q1/6869 ,  B01J2219/00286 ,  B01J2219/00369 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2300/1844 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/143333 ,  Y10T436/2575 ,  C12Q2565/518 ,  C12Q2535/101 ,  C12Q2527/125

摘要： Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.