公开(公告)号：US11807857B2

公开(公告)日：2023-11-07

申请号：US15321969

申请日：2015-06-24

Applicant: Monsanto Technology LLC

Inventor： Michael Bennett ,  Bill L. Hendrix ,  Alberto Iandolino ,  Yao Luo ,  Wei Zheng

IPC: C12N15/82

CPC classification number: C12N15/8206 ,  C12N15/8218 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8285 ,  C12N15/8286

Abstract: Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with ‘one step’ treatment and plant foliar cells with ‘one step’ topical application.

公开(公告)号：US20160060648A1

公开(公告)日：2016-03-03

申请号：US14939691

申请日：2015-11-12

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards Allen ,  Veena S. Anil ,  Amarjit Basra ,  Christopher T. Bauer ,  Prasanna R. Bhat ,  Raghunatha P. Chari ,  Jaishree Chittoor ,  Paul Chomet ,  Karen K. Gabbert ,  Cara Griffith ,  Bill Hendrix ,  Alberto Iandolino ,  Hongwu Jia ,  Kevin R. Kosola ,  Saritha V. Kuriakose ,  Paul J. Loida ,  Linda L. Lutfiyya ,  Mingsheng Peng ,  Ying Peng ,  Bellur Narasimha Prasad ,  Monnanda S. Rajani ,  Monica P. Ravanello ,  Donald Todd ,  Srikanth Babu Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  K. R. Vidya ,  Huai Wang

IPC: C12N15/82

CPC classification number: C12N15/8274 ,  C07K14/415 ,  C12N15/8271 ,  C12N15/8273

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of Arabidopsis thaliana heat stress transcription factor A-2, which is useful for imparting enhanced agronomic traits) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

Abstract translation: 本发明提供具有用于表达拟南芥热胁迫转录因子A-2的重组DNA的转基因植物细胞，其可用于赋予改良的农艺性状）转基因作物植物。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US20160272994A1

公开(公告)日：2016-09-22

申请号：US14999032

申请日：2016-03-21

Applicant: Monsanto Technology LLC

Inventor： Mark Scott Abad ,  Thandoni Rao Ambika ,  Erin Bell ,  Kraig Brustad ,  R. Eric Cerny ,  Shobha Char ,  Jaishree M. Chittoor-Vijayanath ,  Paul S. Chomet ,  Julie Davis ,  Molian Deng ,  Todd DeZwaan ,  Michael D. Edgerton ,  Karen Gabbert ,  Barry S. Goldman ,  Brian Hauge ,  Steve He ,  Jacqueline E. Heard ,  Alberto Iandolino ,  Ping Li ,  Linda L. Lutfiyya ,  Savitha Madappa ,  Brad McDill ,  Robert J. Meister ,  Peter Morrell ,  Anil Neelam ,  Marie Petracek ,  John Pitkin ,  M. S. Rajani ,  Dhanalakshmi Ramachandra ,  Monica P. Ravanello ,  Maria F. Rodriguez ,  Thomas G. Ruff ,  Sangeetha Singh ,  Virginia Ursin ,  Tymagondlu V. Venkatesh ,  Kammaradi R. Vidya ,  K. Vijayalakshmi ,  Xiaoyun Wu ,  Jianmin Zhao

IPC: C12N15/82 ,  C07K14/415 ,  G01N33/68 ,  C12Q1/68

CPC classification number: C12N15/8273 ,  C07K14/415 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12Q1/6895 ,  C12Q2600/13 ,  G01N33/68 ,  G01N2333/415 ,  Y02A40/146

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

公开(公告)号：US20160160212A1

公开(公告)日：2016-06-09

申请号：US14746617

申请日：2015-06-22

Applicant: Monsanto Technology LLC

Inventor： Alberto Iandolino ,  Juan Pedro Sanchez

IPC: C12N15/113 ,  C12N15/82

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N15/8218 ,  C12N2310/14 ,  C12N2320/51 ,  C12N2320/52

Abstract: The present disclosure provides compositions and methods for regulating gene expression via RNA-mediated silencing. The present disclosure also provides compositions and methods to optimize the processing of a dsRNA molecule into small RNA duplexes. The present disclosure further provides compositions and methods to improve the efficiency of a dsRNA molecule in producing desired small RNAs and promoting the silencing of a gene of interest.

Abstract translation: 本公开提供了通过RNA介导的沉默来调节基因表达的组合物和方法。 本公开还提供了优化dsRNA分子加工成小RNA双链体的组合物和方法。 本公开进一步提供组合物和方法，以提高dsRNA分子在产生所需小RNA中的效率并促进感兴趣的基因的沉默。

公开(公告)号：US10988764B2

公开(公告)日：2021-04-27

申请号：US14746617

申请日：2015-06-22

Applicant: Monsanto Technology LLC

Inventor： Alberto Iandolino ,  Juan Pedro Sanchez

IPC: C12N15/113 ,  C12N15/82 ,  C12N15/11

Abstract: The present disclosure provides compositions and methods for regulating gene expression via RNA-mediated silencing. The present disclosure also provides compositions and methods to optimize the processing of a dsRNA molecule into small RNA duplexes. The present disclosure further provides compositions and methods to improve the efficiency of a dsRNA molecule in producing desired small RNAs and promoting the silencing of a gene of interest.