公开(公告)号：US20240124884A1

公开(公告)日：2024-04-18

申请号：US18538893

申请日：2023-12-13

Applicant: Monsanto Technology LLC

Inventor： Stanton B. Dotson ,  Larry A. Gilbertson ,  Jonathan C. Lamb ,  Brenda Lowe ,  Zoe P. McCuddin

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8257 ,  C12N15/8261 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12N15/8289 ,  C12N15/829

Abstract: The invention provides novel uses of sequence-specific or sequence-directed endonucleases for molecular plant breeding. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of an endonuclease with plant expression and transformation elements. Plants and derivatives thereof produced by such methods are also provided.

公开(公告)号：US20180119157A1

公开(公告)日：2018-05-03

申请号：US15720759

申请日：2017-09-29

Applicant: Monsanto Technology LLC

Inventor： Jyoti R. Rout ,  Brenda Lowe ,  John Purcell ,  Anne Spelletich ,  Michael Spencer ,  Melissa Way

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8201 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8265

Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

公开(公告)号：US09783813B2

公开(公告)日：2017-10-10

申请号：US14483748

申请日：2014-09-11

Applicant: Monsanto Technology LLC

Inventor： Jyoti R. Rout ,  Brenda Lowe ,  John Purcell ,  Anne Spelletich ,  Michael Spencer ,  Melissa Way

IPC: C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8205 ,  C12N15/8201 ,  C12N15/8202 ,  C12N15/8206 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8265 ,  C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

公开(公告)号：US20150013034A1

公开(公告)日：2015-01-08

申请号：US14483748

申请日：2014-09-11

Applicant: Monsanto Technology LLC

Inventor： Jyoti R. Rout ,  Brenda Lowe ,  John Purcell ,  Anne Spelletich ,  Michael Spencer ,  Melissa Way

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8201 ,  C12N15/8202 ,  C12N15/8206 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8265 ,  C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

Abstract translation: 本发明提供了通过简单且快速的方案转化单子叶植物的方法，以获得能够在4-8周内种植到土壤中的再生植物。 还提供了相关的细胞培养基和生长条件，以及通过该方法获得的植物和植物部分。 此外，还提供了通过本发明的方法筛选用于可变形性的顽固植物基因型的方法。 此外，还提供了通过本发明的方法扩展用于生产转基因植物的优先发展窗口的系统。