公开(公告)号：US11225672B2

公开(公告)日：2022-01-18

申请号：US16658892

申请日：2019-10-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  C07K14/195 ,  A01N37/46 ,  C07K14/32 ,  A01N63/50

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US20180291395A1

公开(公告)日：2018-10-11

申请号：US15944114

申请日：2018-04-03

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  C07K14/415 ,  C07K14/47 ,  C07K14/32 ,  C07K14/245 ,  C07K14/27 ,  C07K14/26

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US20250160276A1

公开(公告)日：2025-05-22

申请号：US18949035

申请日：2024-11-15

Applicant: Monsanto Technology LLC

Inventor： Krishnakumar Sridharan

IPC: A01H1/00 ,  C12N5/04 ,  C12N15/113

Abstract: Recombinant DNA polynucleotides and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants are provided. Also, transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA polynucleotides operably linked to heterologous transcribable DNA polynucleotides are provided. Also provided are methods of the use of the recombinant DNA polynucleotides and constructs.

公开(公告)号：US12275944B2

公开(公告)日：2025-04-15

申请号：US18501949

申请日：2023-11-03

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US20240352480A1

公开(公告)日：2024-10-24

申请号：US18628578

申请日：2024-04-05

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32 ,  C07K2319/00

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US11859191B2

公开(公告)日：2024-01-02

申请号：US17817196

申请日：2022-08-03

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8205 ,  C12N2310/20 ,  C12N2800/80

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US11414669B2

公开(公告)日：2022-08-16

申请号：US16563581

申请日：2019-09-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US20220235369A1

公开(公告)日：2022-07-28

申请号：US17575318

申请日：2022-01-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flainski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  A01N37/46 ,  C07K14/195 ,  C07K14/32 ,  A01N63/50

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US10465205B2

公开(公告)日：2019-11-05

申请号：US15944114

申请日：2018-04-03

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  C07K14/195 ,  C07K14/32 ,  A01N37/46 ,  A01N63/02

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US11981908B2

公开(公告)日：2024-05-14

申请号：US17575318

申请日：2022-01-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32 ,  C07K2319/00 ,  A01N63/50 ,  A01N63/20

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.