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    Compositions and methods for expression of nitrogenase in plant cells
    4.
    发明授权
    Compositions and methods for expression of nitrogenase in plant cells 有权

    公开(公告)号：US10913939B2

    公开(公告)日：2021-02-09

    申请号：US15080571

    申请日：2016-03-24

    Applicant: Monsanto Technology LLC

    Inventor： William P. Donovan ,  Natalia Ivleva ,  Jeffrey M. Staub

    IPC: C12N15/82 ,  C12N9/02

    Abstract: The disclosure provides nucleic acid constructs encoding novel chimeric peptides that are useful for nuclear and chloroplast expression of active nitrogenase components including dinitrogenase reductase and dinitrogenase, in plant cells. The disclosure thus provides nucleic acid constructs encoding the chimeric proteins, as well as plant expression constructs comprising the same for expression and/or targeting to the nucleus, plastids, or mitochondria of plant cells. The disclosure also provides methods of use for the novel chimeric peptides that function to provide active dinitrogenase reductase, dinitrogenase, and thus nitrogenase, expressed in plant cells, and plants comprising such nucleic acid constructs.

    COMPOSITIONS AND METHODS FOR EXPRESSION OF NITROGENASE IN PLANT CELLS
    6.
    发明申请
    COMPOSITIONS AND METHODS FOR EXPRESSION OF NITROGENASE IN PLANT CELLS 审中-公开
    用于在植物细胞中表达NITROGENASE的组合物和方法

    公开(公告)号：US20160304842A1

    公开(公告)日：2016-10-20

    申请号：US15080571

    申请日：2016-03-24

    Applicant: Monsanto Technology LLC

    Inventor： William P. Donovan ,  Natalia lvleva ,  Jeffrey M. Staub

    IPC: C12N9/02 ,  C12N15/82

    CPC classification number: C12N9/0095 ,  C12N15/8243 ,  C12Y118/06001

    Abstract: The disclosure provides nucleic acid constructs encoding novel chimeric peptides that are useful for nuclear and chloroplast expression of active nitrogenase components including dinitrogenase reductase and dinitrogenase, in plant cells. The disclosure thus provides nucleic acid constructs encoding the chimeric proteins, as well as plant expression constructs comprising the same for expression and/or targeting to the nucleus, plastids, or mitochondria of plant cells. The disclosure also provides methods of use for the novel chimeric peptides that function to provide active dinitrogenase reductase, dinitrogenase, and thus nitrogenase, expressed in plant cells, and plants comprising such nucleic acid constructs.

    Abstract translation: 本公开提供编码新颖嵌合肽的核酸构建体，其可用于植物细胞中用于核和叶绿体表达活性氮酶组分（包括二氮化酶还原酶和二氮解酶）的组合。 因此，本公开提供了编码嵌合蛋白质的核酸构建体，以及包含用于表达和/或靶向植物细胞的细胞核，质体或线粒体的植物表达构建体。 本公开还提供了用于提供在植物细胞中表达的活性二氮酶还原酶，二氮因酶，因此氮酶，以及包含这种核酸构建体的植物的新型嵌合肽的方法。

    Plastid Transformation of Maize
    7.
    发明申请
    Plastid Transformation of Maize 有权
    玉米的质体转化

    公开(公告)号：US20160186193A1

    公开(公告)日：2016-06-30

    申请号：US14988970

    申请日：2016-01-06

    Applicant: Monsanto Technology LLC

    Inventor： Vladimir Sidorov ,  Jeffrey M. Staub ,  Yuechun Wan ,  Guangning Ye

    IPC: C12N15/82

    CPC classification number: C12N15/8214

    Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

    Abstract translation: 提供了用于转化单子叶植物以从植物细胞质体表达目的DNA序列的方法。 该方法允许用异源DNA构建体转化单子叶植物组织。 本发明还提供其中质体含有异源DNA构建体的单子叶植物细胞。

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