公开(公告)号：US20210332375A1

公开(公告)日：2021-10-28

申请号：US17317255

申请日：2021-05-11

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, JR. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

IPC: C12N15/82

Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

公开(公告)号：US20200010838A1

公开(公告)日：2020-01-09

申请号：US16516025

申请日：2019-07-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Whitney R. Adams, JR. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

IPC: C12N15/82

Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

公开(公告)号：US20210219508A1

公开(公告)日：2021-07-22

申请号：US17124032

申请日：2020-12-16

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brian J. Martinell ,  Jyoti R. Rout ,  Brenda A. Lowe

IPC: A01H4/00

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

公开(公告)号：US20200053970A1

公开(公告)日：2020-02-20

申请号：US16553911

申请日：2019-08-28

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brenda A. Lowe ,  Michael Spencer ,  Michael T. Mann

IPC: A01H4/00 ,  A01G7/00 ,  C12N15/82 ,  A01H5/10

Abstract: The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

公开(公告)号：US20170238489A1

公开(公告)日：2017-08-24

申请号：US15452519

申请日：2017-03-07

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brenda A. Lowe ,  Michael Spencer ,  Michael T. Mann

IPC: A01H4/00 ,  A01G7/00 ,  C12N15/82

CPC classification number: A01H4/003 ,  A01G7/00 ,  A01H4/001 ,  A01H4/008 ,  A01H5/10 ,  C12N15/82 ,  C12N15/8201 ,  C12N15/8205

Abstract: The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

公开(公告)号：US20230099076A1

公开(公告)日：2023-03-30

申请号：US17955430

申请日：2022-09-28

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brian J. Martinell ,  Jyoti R. Rout ,  Brenda A. Lowe

IPC: A01H4/00

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

公开(公告)号：US20190082630A1

公开(公告)日：2019-03-21

申请号：US16106342

申请日：2018-08-21

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brian J. Martinell ,  Jyoti R. Rout ,  Brenda A. Lowe

IPC: A01H4/00

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

公开(公告)号：US20150007370A1

公开(公告)日：2015-01-01

申请号：US14314963

申请日：2014-06-25

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brenda A. Lowe ,  Michael Spencer ,  Michael T. Mann

IPC: A01H5/00

CPC classification number: A01H5/00 ,  A01G7/00 ,  A01H4/001 ,  A01H4/003 ,  A01H4/008 ,  A01H5/10 ,  C12N15/82 ,  C12N15/8201 ,  C12N15/8205

Abstract: The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

Abstract translation: 本发明公开并要求用于快速机械分离适于转化或组织培养的单子叶植物组织的方法和装置。 本发明包括用于基本上分离靶植物组织以用作可转化外植体的机械装置，以及转基因植物和植物组织的繁殖。

公开(公告)号：US20150082497A1

公开(公告)日：2015-03-19

申请号：US14339934

申请日：2014-07-24

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brian J. Martinell ,  Jyoti R. Rout ,  Brenda A. Lowe

IPC: A01H4/00

CPC classification number: A01H4/005 ,  A01H4/001

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.